Safe injection of cultured Schwann cells into peripheral nerve allografts

Ogden, Margaret; Feng, Felix Y.; Myckatyn, Terence M.; Jensen, John; Grand, Aaron G.; Wood, Patrick M.; Hunter, Daniel A.; Mackinnon, Susan E.

doi:10.1002/1098-2752(2000)20:7<314::aid-micr2>3.0.co;2-w

Cited by 31 publications

(11 citation statements)

References 61 publications

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“…The experiments of transplanting cultured SCs into peripheral nerve grafts have shown the improvement of nerve regeneration in peripheral nerve repairing (Dubovy, ). However, it remains controversial in clinical application, because of the difficulty of isolation, expansion and purification of SCs in vitro and the immunoreaction of allogenic SCs (Keilhoff et al, ; Ogden et al, ).…”

Section: Discussionmentioning

confidence: 99%

Synergistic effects of ultrashort wave and bone marrow stromal cells on nerve regeneration with acellular nerve allografts

ChaoJian

Tong

et al. 2013

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Acellular nerve allografts (ANA) possess bioactivity and neurite promoting factors in nerve tissue engineering. Previously we reported that low dose ultrashort wave (USW) radiation could enhance the rate and quality of peripheral nerve regeneration with ANA repairing sciatic nerve defects. Meanwhile, ANA implanted with bone marrow stromal cells (BMSCs) exhibited a similar result. Thus, it is interesting to know whether it might yield a synergistic effect when USW radiation is combined with BMSCs-laden ANA. Here we investigated the effectiveness of ANA seeded with BMSCs, combined with USW therapy on repairing peripheral nerve injuries. Adult male Wistar rats were randomly divided into four groups: Dulbecco's modified Eagle's medium (DMEM) control group, BMSCs-laden group, ultrashort wave (USW) group and BMSC + USW group. The regenerated nerves were assayed morphologically and functionally, and growth-promoting factors in the regenerated tissues following USW administration or BMSCs integration were also detected. The results indicated that the combination therapy caused much better beneficial effects evidenced by increased myelinated nerve fiber number, myelin sheath thickness, axon diameter, sciatic function index, nerve conduction velocity, and restoration rate of tibialis anterior wet weight. Moreover, the mRNA levels of brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) in the spinal cord and muscles were elevated significantly. In conclusion, we found a synergistic effect of USW radiation and BMSCs treatment on peripheral nerve regeneration, which may help establish novel strategies for repairing peripheral nerve defects.

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Section: Discussionmentioning

confidence: 99%

Synergistic effects of ultrashort wave and bone marrow stromal cells on nerve regeneration with acellular nerve allografts

ChaoJian

Tong

et al. 2013

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“…Autologous transplantation of primary SCs has, therefore, been used to enhance nerve regeneration in animal models of peripheral nerve injury [71, 87, 105–112]. SCs isolated from nerve tissue and cultured in vitro retain the ability to express trophic factors to support axonal regeneration and to myelinate axons following regeneration [97, 108, 109, 113–116, 117–119]. Mitogens can be employed during the culture process to expand cultured SC populations and thus increase the number of cells needed for transplantation [120, 121].…”

Section: Cellular Component Of Tissue Engineered Peripheral Nervementioning

confidence: 99%

“…SCs in culture have also been shown to not transform or reach a proliferative limit that would alter their utility for later transplantation [122]. Most importantly, expanded human and rat SC populations have not demonstrated tumor formation when transplanted into rodents after mitogen removal [108, 123]. …”

Section: Cellular Component Of Tissue Engineered Peripheral Nervementioning

confidence: 99%

“…Even with optimization, the expansion of SCs requires 6–10 weeks from the time of harvest. Thus, the combined barrier of donor site morbidity and difficulty of effective SC isolation and culture has significantly impeded clinical translation despite decades of concerted interest and effort [71, 108, 109]. Only recently, after over 30 years of research, SCs were employed in a clinical trial as a treatment for spinal cord injury by the researchers of the Miami Project to Cure Paralysis.…”

Section: Cellular Component Of Tissue Engineered Peripheral Nervementioning

confidence: 99%

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Tissue engineered constructs for peripheral nerve surgery

et al. 2013

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Summary Background Tissue engineering has been defined as “an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function or a whole organ”. Traumatic peripheral nerve injury resulting in significant tissue loss at the zone of injury necessitates the need for a bridge or scaffold for regenerating axons from the proximal stump to reach the distal stump. Methods A review of the literature was used to provide information on the components necessary for the development of a tissue engineered peripheral nerve substitute. Then, a comprehensive review of the literature is presented composed of the studies devoted to this goal. Results Extensive research has been directed toward the development of a tissue engineered peripheral nerve substitute to act as a bridge for regenerating axons from the proximal nerve stump seeking the distal nerve. Ideally this nerve substitute would consist of a scaffold component that mimics the extracellular matrix of the peripheral nerve and a cellular component that serves to stimulate and support regenerating peripheral nerve axons. Conclusions The field of tissue engineering should consider its challenge to not only meet the autograft “gold standard” but also to understand what drives and inhibits nerve regeneration in order to surpass the results of an autograft.

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“…On the other hand, cultured Schwann cells have been used as transplants 14–16. Because there is a problem of the immunoreactions of allogenous Schwann cells,17 we have searched the other cell as a source of Schwann cell.…”

Section: Introductionmentioning

confidence: 99%

Peripheral nerve regeneration by transplantation of BMSC‐derived Schwann cells as chitosan gel sponge scaffolds

Ishikawa

Suzuki

Dezawa

et al. 2009

J Biomedical Materials Res

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It is said that bone marrow stromal cells (BMSCs) are able to differentiate into different kinds of cells, including Schwann cells, under relevant conditions (Dezawa et al., Eur J Neurosci 2001;14:1771-1776). In the previous paper, we demonstrated that chitosan gel sponge is one of the effective scaffolds for regenerating axons of the rat sciatic nerve (Ishikawa et al., J Biomed Mater Res A 2007;83:33-40). In the present study, we examined whether BMSC-derived Schwann cells with chitosan gel sponges were one of the effective scaffolds for peripheral nerve regeneration in rats. BMSC-derived cells with Schwann cell characteristics were labeled by green fluorescent protein using a retrovirus. An 8-mm gap was made by removing a nerve segment from the rat peripheral nerve, and chitosan gel sponges containing BMSC-derived Schwann cells were grafted, sandwiching the proximal and distal stumps of the transected nerve. Rats were sacrificed at 7, 14, and 28 days, and 2 and 4 months after transplantation. Immunohistochemistry demonstrated that regenerating axons were found near transplanted Schwann cells 7 days after surgery and extended into the host distal nerve segment at 14 days after surgery. Electron microscopy showed that transplanted Schwann cells formed myelin sheaths on regenerating axons 1 month after transplantation. The mean diameter of myelinated fibers was increased from 2.58 mum at 2 months to 2.84 mum at 4 months postsurgery. This study indicates that chitosan gel sponges containing BMSC-derived Schwann cells have strong potentiality as a graft that can be used for peripheral nerve regeneration.

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Safe injection of cultured Schwann cells into peripheral nerve allografts

Cited by 31 publications

References 61 publications

Synergistic effects of ultrashort wave and bone marrow stromal cells on nerve regeneration with acellular nerve allografts

Synergistic effects of ultrashort wave and bone marrow stromal cells on nerve regeneration with acellular nerve allografts

Tissue engineered constructs for peripheral nerve surgery

Peripheral nerve regeneration by transplantation of BMSC‐derived Schwann cells as chitosan gel sponge scaffolds

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