Safe and rapid development of capillary electrophoresis for ultratrace uranyl ions in radioactive samples by way of fluorescent probe selection for actinide ions from a chemical library

Purification of anionic fluorescent probes through precise fraction collection with a two‐point detection system using multiple‐stacking preparative capillary transient isotachophoresis

“…FTC‐ABNOTA was synthesized by Dojindo according to a previously reported method [33] and purified using HPLC (92.9% purity). FTC‐PDA was synthesized by Sapala Organics (Hyderabad, India) according to a previously reported method [35] and purified using HPLC (97.9% purity). A labeled fluorescent DNA aptamer (5’‐FAM/AGT CCG TGG TAG GGC AGG TTG GGG TGA CT‐3’, F‐29mer) was purchased from Integrated DNA Technology (Coralville, Iowa, USA).…”

“…We previously developed the fluorescent metal probes FTC-ABNOTA and FTC-PDA for CE-LIF determination of trace Nd 3+ [34] and UO 2 2+ [35] in spent nuclear fuel and radioactive waste samples. However, impurities generated peaks that eluted near those of the target Nd 3+ -FTC-ABNOTA and UO 2 2+ -FTC-PDA complexes.…”

“…We previously developed several fluorescent probes for the selective CE-LIF detection [29][30][31][32] of ultratrace Nd 3+ [33,34] and UO 2 2+ ions [35]. Some of these probes were synthesized by a chemical supplier using the protocol given in our previous papers [33,35] and were further purified using chromatographic techniques (Section 2). However, CE-LIF detection is often impeded by interferences caused by impurities.…”

“…For example, for the CE-LIF detection of ultratrace UO 2 2+ ions, targets (UO 2 2+ -probe complex) and impurities were concentrated in the same peak by the ITP procedure. Furthermore, the targets and impurities could not be separated using a combination of ITP and CZE [35]. The exceptional resolution and high sensitivity of CE-LIF thus enable detection of these trace impurities.…”

(E)-6,6′-(Diazene-1,2-diyl)bis(1,10-phenanthrolin-5-ol) trichloromethane disolvate: a superconjugated ligand

“…The chemical versatility of 1,10-phenanthroline (phen), its substituted derivatives and corresponding metal complexes (Bencini & Lippolis, 2010) is exemplified by their uses as organic light-emitting diodes (OLED)/electroluminescent display and solid-state lighting materials (Li et al, 2009), fluorescence molecular probes and imaging agents (Haraga et al, 2018), ion sensors (Zheng et al, 2012), solar energy converters (Freitag et al, 2016), anti-cancer and antimicrobial cytotoxins (McCann et al, 2012), DNA/RNA binding/cleavage (Kellett et al, 2011), enzyme inhibitors (Zhu et al, 2015), biomimetics (Casey et al, 1994) and catalysts (Lu et al, 2015). Given the resourcefulness of phenanthrolines, there is a continuing demand for new molecules containing this structural motif.…”