Safe and genotyped. A non-invasive method for extraction of amphibian DNA from water baths and its application on Northern spectacled salamanders, Salamandrina perspicillata (Savi 1821)
Leonardo Brustenga,
Antonio Romano,
Gianandrea La Porta
et al.
Abstract:The global decline of amphibian populations, with 40.7% of species classified as threatened, calls for innovative and ethical approaches in conservation genetics. Molecular biology advancements have introduced environmental DNA (eDNA) analysis, primarily focusing on aquatic environments. However, the present study explores a novel non-invasive protocol using water samples to extract DNA from terrestrial and semi-terrestrial amphibians, specifically the endangered Italian endemic salamander, Salamandrina perspi… Show more
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