Safe and cost-effective rapid-prototyping of multilayer PMMA microfluidic devices

Liga, Antonio; Morton, Jonathan; Kersaudy-Kerhoas, Maïwenn

doi:10.1007/s10404-016-1823-1

Cited by 56 publications

(41 citation statements)

References 40 publications

(40 reference statements)

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“…Solvent-assisted thermal bonding exploits the use of plasticisers, which reduce the glass transition temperature of the thermoplastic surface, to achieve thermal bonding at lower temperatures and shorter times. Ethanol 18 , chloroform 19 , dibutyl phthalate (DBP) 20 , and DMSO/Methanol 21 have been reported as effective plasticisers for rapid solvent-assisted thermoplastic bonding. While these methods allow for efficient assembly of thermoplastic devices without causing bulk distortion, they present some limitations.…”

Section: Introductionmentioning

confidence: 99%

Cost-effective rapid prototyping and assembly of poly(methyl methacrylate) microfluidic devices

Matellan

Hernández

2018

Sci Rep

103

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The difficulty in translating conventional microfluidics from laboratory prototypes to commercial products has shifted research efforts towards thermoplastic materials for their higher translational potential and amenability to industrial manufacturing. Here, we present an accessible method to fabricate and assemble polymethyl methacrylate (PMMA) microfluidic devices in a “mask-less” and cost-effective manner that can be applied to manufacture a wide range of designs due to its versatility. Laser micromachining offers high flexibility in channel dimensions and morphology by controlling the laser properties, while our two-step surface treatment based on exposure to acetone vapour and low-temperature annealing enables improvement of the surface quality without deformation of the device. Finally, we demonstrate a capillarity-driven adhesive delivery bonding method that can produce an effective seal between PMMA devices and a variety of substrates, including glass, silicon and LiNbO3. We illustrate the potential of this technique with two microfluidic devices, an H-filter and a droplet generator. The technique proposed here offers a low entry barrier for the rapid prototyping of thermoplastic microfluidics, enabling iterative design for laboratories without access to conventional microfabrication equipment.

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Section: Introductionmentioning

confidence: 99%

Cost-effective rapid prototyping and assembly of poly(methyl methacrylate) microfluidic devices

Matellan

Hernández

2018

Sci Rep

103

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“…To enhance diazirine photoactivation efficiency we constructed a 365 nm LED lamp (Figure c, Figure S4a,b) with a nominal flux density of 15 W cm −2 . We designed devices to irradiate samples in 0.2 mL to 1.5 mL tubes, or in acrylic chips with 33 μL sample wells (Figure c, Figure S5). Using this LED platform, maximal XL yields were achieved in 10 seconds, with an associated heating of <2 °C (Figure a,b, Figure S4c).…”

Section: Figurementioning

confidence: 89%

Rapid Mapping of Protein Interactions Using Tag‐Transfer Photocrosslinkers

et al. 2018

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Analysing protein complexes by chemical crosslinking‐mass spectrometry (XL‐MS) is limited by the side‐chain reactivities and sizes of available crosslinkers, their slow reaction rates, and difficulties in crosslink enrichment, especially for rare, transient or dynamic complexes. Here we describe two new XL reagents that incorporate a methanethiosulfonate (MTS) group to label a reactive cysteine introduced into the bait protein, and a residue‐unbiased diazirine‐based photoactivatable XL group to trap its interacting partner(s). Reductive removal of the bait transfers a thiol‐containing fragment of the crosslinking reagent onto the target that can be alkylated and located by MS sequencing and exploited for enrichment, enabling the detection of low abundance crosslinks. Using these reagents and a bespoke UV LED irradiation platform, we show that maximum crosslinking yield is achieved within 10 seconds. The utility of this “tag and transfer” approach is demonstrated using a well‐defined peptide/protein regulatory interaction (BID80‐102/MCL‐1), and the dynamic interaction interface of a chaperone/substrate complex (Skp/OmpA).

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“…The large recast layer produced in the laser-cut PLA sample makes this material impractical for use in the context of a layerby-layer prototyping technique due to a reduced surface contact during the assembly. However, we hypothesised that the PLA polymer structure could be manipulated to reduce the HAZ and, in particular the recast layer, so as to produce better cut quality, compatible with a layer-by-layer assembly developed in (Liga 2016). To investigate this hypothesis, four types of PLA sheets were produced with differing degrees of crystallinity using a compression moulding technique: Ultra High Cooling Rate (UHCR), High Cooling Rate (HCR), Low Cooling Rate (LCR) and Ultra Low Cooling Rate (ULCR) (Suppl.…”

Section: Investigating the Laser Workability Of Pla With Different Crmentioning

confidence: 99%

“…Furthermore, advances in multilayer rapid-prototyping techniques have permitted the manufacturing of complex microfluidic devices in less than 15 minutes from design to test. 21 This method involves laser cutting individual layers followed by multi-layer bonding using a solvent assisted method. Here, we propose a design for sustainability (DFS) approach 22 for the production of environmentally friendly medical microcomponents ( Figure 1A).…”

Section: Introductionmentioning

confidence: 99%

Laser Ablation of Poly(lactic acid) Sheets for the Rapid Prototyping of Sustainable, Single-Use, Disposable Medical Microcomponents

Ongaro

Keraite

Liga

et al. 2018

ACS Sustainable Chem. Eng.

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Safe and cost-effective rapid-prototyping of multilayer PMMA microfluidic devices

Cited by 56 publications

References 40 publications

Cost-effective rapid prototyping and assembly of poly(methyl methacrylate) microfluidic devices

Cost-effective rapid prototyping and assembly of poly(methyl methacrylate) microfluidic devices

Rapid Mapping of Protein Interactions Using Tag‐Transfer Photocrosslinkers

Laser Ablation of Poly(lactic acid) Sheets for the Rapid Prototyping of Sustainable, Single-Use, Disposable Medical Microcomponents

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