Safe Administration of Carbon Nanotubes by Intravenous Pathway in BALB/c Mice

Guzmán-Mendoza, José Jesús; Montes-Fonseca, Silvia Lorena; Ramos-Martínez, Ernesto; González-Horta, Carmen; Hernández-Rodríguez, Pilar del Carmen; Orrantia‐Borunda, Erasmo; Chávez‐Flores, David; Sánchez‐Ramírez, Blanca

doi:10.3390/nano10020400

Cited by 11 publications

(3 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…31 Guzmán-Mendoza et al reported functionalization-dependent toxicity of MWCNTs after intravenous injection of mice with 2 mg/kg bodyweight NMs. 32 Therefore, the concentration of MWCNTs used in this study is comparable to previous studies. For HNTs, relatively few studies investigated the toxicity of HNTs after intravenous injection, but some studies investigated the toxicity HNTs to mice via other exposure routes.…”

Section: Intravenous Injection For Micesupporting

confidence: 77%

Effects of multi‐walled carbon nanotubes and halloysite nanotubes on plasma lipid profiles and autophagic lipolysis pathways in mouse aortas and hearts

Cheng,

Zhang,

Wang

et al. 2024

Environmental Toxicology

View full text Add to dashboard Cite

Multi‐walled carbon nanotubes (MWCNTs) and halloysite nanotubes (HNTs) are widely used tubular‐structured nanomaterials (NMs), but their cardiovascular effects are not clear. This study compared the effects of MWCNTs and HNTs on lipid profiles in mouse plasma and gene expression profiles in aortas and hearts. Mice were intravenously injected with 50 μg NMs, once a day, for 5 days. Then, the plasma was collected for lipidomics analysis, and aortas and hearts were collected for RNA‐sequencing analysis. While MWCNTs or HNTs did not induce obvious pathological changes in aortas or hearts, the lipid profiles in mouse plasma were altered. Further analysis revealed that MWCNTs more effectively upregulated sphingolipids and sterol lipids, whereas HNTs more effectively upregulated glycerophospholipids and fatty acyls. Consistently, RNA‐sequencing data indicated that MWCNTs and HNTs altered signaling pathways related with lipid synthesis and metabolism, as well as those related with endoplasmic reticulum, lysosomes and autophagy, more significantly in aortas than in hearts. We further verified the changes of proteins involved in autophagic lipolysis, that MWCNTs were more effectively to suppress the autophagic biomarker LC3, whereas HNTs were more effectively to affect lipid metabolism proteins. These results may provide novel understanding about the influences of MWCNTs and HNTs on lipid profiles and lipid signaling pathways in cardiovascular systems. Importantly, previous studies considered HNTs as biocompatible materials, but the results from this study suggested that both MWCNTs and HNTs were capable to affect lipid profiles and autophagic lipolysis pathways in cardiovascular systems, although their exact influences were different.

show abstract

Section: Intravenous Injection For Micesupporting

confidence: 77%

Effects of multi‐walled carbon nanotubes and halloysite nanotubes on plasma lipid profiles and autophagic lipolysis pathways in mouse aortas and hearts

Cheng,

Zhang,

Wang

et al. 2024

Environmental Toxicology

View full text Add to dashboard Cite

show abstract

“…MWCNTs were administrated intravenously, and biochemical and histopathological parameters were analyzed at 1, 14, 29, and 60 days post-exposure. Pristine MWCNTs have shown the highest toxicity, especially due to accumulation in the kidneys and the lungs even at 60 days [ 93 ]. Tang et al studied short-term and long-term toxicity of multi-walled carbon nanotubes.…”

Section: Nephrotoxicitymentioning

confidence: 99%

Assessment of Pristine Carbon Nanotubes Toxicity in Rodent Models

Witkowska

Florek

Mrówczyński

2022

IJMS

View full text Add to dashboard Cite

Carbon nanotubes are increasingly used in nanomedicine and material chemistry research, mostly because of their small size over a large surface area. Due to their properties, they are very attractive candidates for use in medicine and as drug carriers, contrast agents, biological platforms, and so forth. Carbon nanotubes (CNTs) may affect many organs, directly or indirectly, so there is a need for toxic effects evaluation. The main mechanisms of toxicity include oxidative stress, inflammation, the ability to damage DNA and cell membrane, as well as necrosis and apoptosis. The research concerning CNTs focuses on different animal models, functionalization, ways of administration, concentrations, times of exposure, and a variety of properties, which have a significant effect on toxicity. The impact of pristine CNTs on toxicity in rodent models is being increasingly studied. However, it is immensely difficult to compare obtained results since there are no standardized tests. This review summarizes the toxicity issues of pristine CNTs in rodent models, as they are often the preferred model for human disease studies, in different organ systems, while considering the various factors that affect them. Regardless, the results showed that the majority of toxicological studies using rodent models revealed some toxic effects. Even with different properties, carbon nanotubes were able to generate inflammation, fibrosis, or biochemical changes in different organs. The problem is that there are only a small amount of long-term toxicity studies, which makes it impossible to obtain a good understanding of later effects. This article will give a greater overview of the situation on toxicity in many organs. It will allow researchers to look at the toxicity of carbon nanotubes in a broader context and help to identify studies that are missing to properly assess toxicity.

show abstract

“… 131 Synthesis methods that focus on the preparation of CNTs with better physicochemical properties are needed to accomplish this. 132 Also, the compatibility of CNTs can be improved by creating oxidized tubes that exhibit enhanced biodegradative potential and limited biopersistence.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Carbon Nanotubes as Carriers in Drug Delivery for Non-Small Cell Lung Cancer, Mechanistic Analysis of Their Carcinogenic Potential, Safety Profiling and Identification of Biomarkers

Pu¹,

Wei²,

Sun³

et al. 2022

IJN

View full text Add to dashboard Cite

Non-small cell lung cancer (NSCLC) is a global burden leading to millions of deaths worldwide every year. Nanomedicine refers to the use of materials at the nanoscale for drug delivery and subsequent therapeutic approaches in cancer. Carbon nanotubes (CNTs) are widely used as nanocarriers for therapeutic molecules such as plasmids, siRNAs, antisense agents, aptamers and molecules related to the immunotherapy for several cancers. They are usually functionalized and loaded with standard drug molecules to improve their therapeutic efficiency. Functionalization and drug loading possibly decrease the genotoxic and carcinogenic potential of CNTs. In addition, the targeted cytotoxic properties of the drug improve and undesired toxicity decreases after drug loading and/or conjugation with proteins, including antibodies. For intended drug delivery, a lysosomal pH of 5.5 is more suitable and effective for the slow and extended release of cytotoxic drugs than a physiological of pH 7.4. Remarkably, CNTs possess intrinsic antitumor properties and are usually internalized by endocytosis. After being internalized, several mechanisms are involved in the therapeutic and carcinogenic effects of CNTs. They are generally safe for therapy, and their toxicity profile remains dependent on their physicochemical properties. Moreover, the dose, route, duration of exposure, surface properties and degradative potential determine the toxicity outcomes of CNTs locally or systemically. In summary, the use of CNTs in drug delivery and NSCLC therapy, as well as their genotoxic and carcinogenic potential and the possible mechanisms, has been discussed in this review. The therapeutic index is generally high for NSCLC cells treated with drug-loaded CNTs; therefore, they are effective carriers in implementing targeted therapy for NSCLC.

show abstract

Safe Administration of Carbon Nanotubes by Intravenous Pathway in BALB/c Mice

Cited by 11 publications

References 45 publications

Effects of multi‐walled carbon nanotubes and halloysite nanotubes on plasma lipid profiles and autophagic lipolysis pathways in mouse aortas and hearts

Effects of multi‐walled carbon nanotubes and halloysite nanotubes on plasma lipid profiles and autophagic lipolysis pathways in mouse aortas and hearts

Assessment of Pristine Carbon Nanotubes Toxicity in Rodent Models

Carbon Nanotubes as Carriers in Drug Delivery for Non-Small Cell Lung Cancer, Mechanistic Analysis of Their Carcinogenic Potential, Safety Profiling and Identification of Biomarkers

Contact Info

Product

Resources

About