Sacrificial crystal templating of hyaluronic acid-based hydrogels

Thomas, Richelle; Chung, Paul; Modi, Shan P.; Hardy, John G.; Schmidt, Christine E.

doi:10.1016/j.eurpolymj.2016.10.022

Cited by 11 publications

(22 citation statements)

References 54 publications

(68 reference statements)

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“…The bulk mechanical properties of hydrogel scaffolds play a role in their performance in vivo, and rheological experiments show that the storage modulus, G', of not templated gels was ca. 100 Pa., by comparison with 0.1 Pa for urea templated gels or 0.6-8.5 Pa or potassium dihydrogen phosphate templated gels, respectively; 41 and therefore that templation offers a method of tuning the mechanics of the gels to suit a specific biological niche. 41 Moreover, we have previously shown that the not templated and templated hydrogels were non-cytotoxic towards fibroblasts and Schwann cells in vitro.…”

Section: Resultsmentioning

confidence: 99%

“…100 Pa., by comparison with 0.1 Pa for urea templated gels or 0.6-8.5 Pa or potassium dihydrogen phosphate templated gels, respectively; 41 and therefore that templation offers a method of tuning the mechanics of the gels to suit a specific biological niche. 41 Moreover, we have previously shown that the not templated and templated hydrogels were non-cytotoxic towards fibroblasts and Schwann cells in vitro. 41 NPCs are particularly interesting for neural tissue engineering applications, and with a view to the use of the hydrogels for such applications we investigated the growth of NPCs within the starburst-like voids in the potassium dihydrogen phosphate-templated hydrogels.…”

Section: Resultsmentioning

confidence: 99%

“…18% of the disaccharides in hyaluronic acid as confirmed by 1 H NMR, displayed in Figure S1. 10,41,42 Hydrogel Preparation. Hydrogels were prepared in accordance with our previously described methodology.…”

Section: Preparation Of Hyaluronic Acid Derivativementioning

confidence: 99%

“…Hydrogels were prepared in accordance with our previously described methodology. 41 In short: hydrogels with 20 mg/mL methacrylated hyaluronic acid and with 0.3% (w/v) photoinitiator (optionally including potassium dihydrogen phosphate [300 mg/ml] or urea [1400 mg/ml]) were mixed at ca. 50 °C.…”

Section: Preparation Of Hyaluronic Acid Derivativementioning

confidence: 99%

“…10 Photocrosslinking the hyaluronic acid derivative yields a continuous polymer network that allows the sacrificial crystal porogens to be removed by leaching in water, leaving a characteristic pore structure within 2D 10 and 3D hydrogels. 41 The ability of these materials to act as scaffolds for nerve tissue regeneration are reported herein.…”

Section: Introductionmentioning

confidence: 99%

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Sacrificial Crystal Templated Hyaluronic Acid Hydrogels As Biomimetic 3D Tissue Scaffolds for Nerve Tissue Regeneration

Thomas

Modi³

et al. 2017

ACS Biomater. Sci. Eng.

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Pores are key features of natural tissues and the development of tissues scaffolds with biomimetic properties (pore structures and chemical/mechanical properties) offers a route to engineer implantable biomaterials for specific niches in the body. Here we report the use of sacrificial crystals (potassium dihydrogen phosphate or urea) that act as templates to impart pores to hyaluronic acid-based hydrogels. The mechanical properties of the hydrogels were analogous to the nervous system (in the Pascal regime), and we investigated the use of the potassium dihydrogen phosphate crystaltemplated hydrogels as scaffolds for neural progenitor cells (NPCs), and the use of urea crystal-templated hydrogels as scaffolds for Schwann cells. For NPCs cultured inside the porous hydrogels, assays for the expression of Nestin are inconclusive, assays for GFAP and BIII-tubulin expression suggest that the NPCs maintain their undifferentiated phenotype more effectively than the controls (with glial fibrillary acidic protein (GFAP) and BIII-tubulin expression at ca. 50% relative to the chemically/mechanically equivalent not templated control hydrogels). For Schwann cells cultured within these hydrogels, assays for the expression of S100 protein or Myelin basic protein confirm the expression of both proteins, albeit at lower levels on the templated hydrogels (ca. 50%) than on the chemically/mechanically equivalent not templated control hydrogels. Such sacrificial crystal templated hydrogels represent platforms for biomimetic 3D tissue scaffolds for the nervous system.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Preparation Of Hyaluronic Acid Derivativementioning

confidence: 99%

Section: Preparation Of Hyaluronic Acid Derivativementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sacrificial Crystal Templated Hyaluronic Acid Hydrogels As Biomimetic 3D Tissue Scaffolds for Nerve Tissue Regeneration

Thomas

Modi³

et al. 2017

ACS Biomater. Sci. Eng.

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Tissue‐Engineered Peripheral Nerve Interfaces

Spearman

Desai

Mobini

et al. 2017

Adv Funct Materials

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Research on neural interfaces has historically concentrated on development of systems for the brain; however, there is increasing interest in peripheral nerve interfaces (PNIs) that could provide benefit when peripheral nerve function is compromised, such as for amputees. Efforts focus on designing scalable and high-performance sensory and motor peripheral nervous system interfaces. Current PNIs face several design challenges such as undersampling of signals from the thousands of axons, nerve-fiber selectivity, and device-tissue integration. To improve PNIs, several researchers have turned to tissue engineering. Peripheral nerve tissue engineering has focused on designing regeneration scaffolds that mimic normal nerve extracellular matrix composition, provide advanced microarchitecture to stimulate cell migration, and have mechanical properties like native nerve. By combining PNIs with tissue engineering, the goal is to promote natural axon regeneration into the devices to facilitate close contact with electrodes; in contrast, traditional PNIs rely on insertion or placement of electrodes into or around existing nerve, or do not utilize materials to actively facilitate axon regeneration. This review presents the state-of-the-art of PNIs and nerve tissue engineering, highlights recent approaches to combine neural interface technology and tissue engineering, and addresses remaining challenges with foreign body response.

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Sacrificial biomaterials in 3D fabrication of scaffolds for tissue engineering applications

Wang,

Zhou

2023

J Biomed Mater Res

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Three‐dimensional (3D) printing technology has progressed exceedingly in the area of tissue engineering. Despite the tremendous potential of 3D printing, building scaffolds with complex 3D structure, especially with soft materials, still exist as a challenge due to the low mechanical strength of the materials. Recently, sacrificial materials have emerged as a possible solution to address this issue, as they could serve as temporary support or templates to fabricate scaffolds with intricate geometries, porous structures, and interconnected channels without deformation or collapse. Here, we outline the various types of scaffold biomaterials with sacrificial materials, their pros and cons, and mechanisms behind the sacrificial material removal, compare the manufacturing methods such as salt leaching, electrospinning, injection‐molding, bioprinting with advantages and disadvantages, and discuss how sacrificial materials could be applied in tissue‐specific applications to achieve desired structures. We finally conclude with future challenges and potential research directions.

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Sacrificial crystal templating of hyaluronic acid-based hydrogels

Abstract: 2016-11-03T14:11:40

Cited by 11 publications

References 54 publications

Sacrificial Crystal Templated Hyaluronic Acid Hydrogels As Biomimetic 3D Tissue Scaffolds for Nerve Tissue Regeneration

Sacrificial Crystal Templated Hyaluronic Acid Hydrogels As Biomimetic 3D Tissue Scaffolds for Nerve Tissue Regeneration

Tissue‐Engineered Peripheral Nerve Interfaces

Sacrificial biomaterials in 3D fabrication of scaffolds for tissue engineering applications

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