Saccharothrix stipae sp. nov., an actinomycete isolated from the rhizosphere of Stipa grandis

Lin, Yan; Guo, Yang; Di, Xiao Hui; Fan, Miao Chun; Dong, Dan Hong; Wei, Ge Hong

doi:10.1099/ijsem.0.000827

Cited by 8 publications

(2 citation statements)

References 34 publications

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“…The genus Saccharothrix belongs to the family Pseudonocardiaceae and was described for the first time by Labeda et al [1]. Since then, Saccharothrix had been found in a variety of environmental components including sediment sample [2], soil [3], endophyte [4] and lake [5]. At the time of writing, LPSN [6] had listed 21 species with validly published names (https://lpsn.dsmz.de/genus/saccharothrix), including the recently described Saccharothrix deserti [7].…”

Section: Full-textmentioning

confidence: 99%

Saccharothrix luteola sp. nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix

Sun

Huang

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach. Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %). Physiological and chemotaxonomic characteristics of the strain further supported its affiliation to the genus Saccharothrix . The whole-cell sugars contained galactose, ribose and mannose. The polar lipids contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant menaquinones were MK-9(H0), MK-9(H2), MK-9(H4) and MK-10(H4). The major fatty acids were iso-C16 : 0, C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0. The genomic DNA G+C content was 71.8 mol%. The levels of digital DNA–DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1 % (37.6–42.6 %), 38.soap8 % (36.3–41.3 %) and 44.8 % (42.2–47.3 %) and the ANI values between them were determined to be 90.2, 89.8 and 91.7 %, the results indicated that strain NEAU-S10T could be distinguished from its reference strains. The assembled genome sequence of strain NEAU-S10T was found to be 10 305 394 bp long. The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) revealed 8 994 protein-coding genes. Genomic analysis and Congo red staining test indicated that strain NEAU-S10T had the potential to degrade cellulose. The genomic and phenotypic results indicate that strain NEAU-S10T represents a novel species of the genus Saccharothrix , for which the name Saccharothrix luteola sp. nov. is proposed, with NEAU-S10T (=CCTCC AA 2020037T=JCM 34800T) as the type strain.

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Section: Full-textmentioning

confidence: 99%

Saccharothrix luteola sp. nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix

Sun

Huang

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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“…Several Saccharothrix species have been described in the past few years. Saccharothrix stipae (18) and Saccharothrix deserti (19) isolated from the soil collected from northwest China where the study revealed that the strain is aerobic, Gram-positive mesophilic actinobacteria that is identified by 16S rRNA and the S. stipae strain contains major meso-diaminopimelic acid and major amino acids are alanine, glycine, glutamic acid, and mannose, rhamnose and galactose were specified by analysis of whole-cell sugars. S. deserti strain cell wall hydrolysates contained mesodiaminopimelic acid, galactose, and mannose.…”

Section: The Genus Saccharothrixmentioning

confidence: 99%

Taxonomy and biological activities of a novel Saccharothrix species from soil of Sichang Island

Azad

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Actinobacteria are the sources of bioactive natural products. Rare actinobacteria are the less frequently isolated genera of actinobacteria. During an investigation of diversity of rare actinobacteria, actinobacteria isolate SC076T was isolated from a soil sample collected from Sichang Island, Chonburi Province, Thailand. The strain showed the highest 16S rRNA gene similarity to Saccharothrix australiensis DSM 43800T (98.58%) and Saccharothrix espanaensis DSM 44229T (98.58%). The zigzag morphology of the spore chain was observed on the aerial mycelia. meso-diaminopimelic acid was detected in the peptidoglycan. Whole-cell sugars contained rhamnose, ribose, mannose glucose and galactose. Polar lipids are phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, phosphatidyl inositol, unidentified ninhydrin positive glycolipid, unidentified glycolipid and four unidentified lipids. Menaquinones are MK-9(H4), MK-9(H8), MK-9(H2) and MK-9(H0). The predominant cellular fatty acids were iso-C16:0, iso-C15:0, and anteiso-C17:0. The strain showed both dDNA-DNA hybridization value and average nucleotide identity of 77.7 82.5% and 84.9 86.7%, respectively. Moreover, the strain showed antimicrobial activity against gram positive pathogenic organisms but did not produce any antifungal activity. For the anticancer activity screening, MTT assay showed IC50 value 126.668 ?g/mL against non-small lung cancer H460 cells. In the higher dose strain showed cytotoxic effect. Unfortunately, the strain did not have potential cell migration and cell invasion effects. Besides, antioxidant and anticholinease activity also tested but no activity represented from the strain. However, from the secondary metabolite profiling from the whole genome sequences it showed 100% similarity with Terpene, PKS compound. Besides chemical profiling of the crude extract determines the strain contains some antibiotic potential chemical compound that will be isolated and purified as further study. Based on the taxonomic evidence, strain SC076T represents a novel species of the genus Saccharothrix for which the name Saccharothrix oblica sp. nov. is proposed. Type strain is SC076T (=TBRC 14540T =NBRC 115117T). In conclusion, these finding ensured that strain SC076T is the novel species of genus Saccharothrix and possesses antibacterial activity as well as cytotoxic effects. The Actinobacteria isolated from soil sample provided biological activity information in drug discovery as the good candidates for the development of antibiotics.� �

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Saccharothrix tharensis sp. nov., an actinobacterium isolated from the Thar Desert, India

Ibeyaima

Singh

Lal

et al. 2018

Antonie van Leeuwenhoek

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The taxonomic provenance of a filamentous actinobacterial strain isolated from a desert soil was established using a polyphasic approach. The strain has chemotaxonomic and morphological properties consistent with its classification in the genus Saccharothrix. It forms a distinct branch in the Saccharothrix 16S rRNA gene tree, related to the type strain of Saccharothrix saharensis (96.7%) but was distinguished readily from it using a combination of phenotypic properties. The genotypic and phenotypic data show that the strain represents a novel species in the genus Saccharothrix, for which the name Saccharothrix tharensis sp. nov. is proposed with the type strain TD-093 (= KCTC 39724 = MCC 2832).

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Saccharothrix stipae sp. nov., an actinomycete isolated from the rhizosphere of Stipa grandis

Cited by 8 publications

References 34 publications

Saccharothrix luteola sp. nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix

Saccharothrix luteola sp. nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix

Taxonomy and biological activities of a novel Saccharothrix species from soil of Sichang Island

Saccharothrix tharensis sp. nov., an actinobacterium isolated from the Thar Desert, India

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