Saccharomyces cerevisiae strain expressing a plant fatty acid desaturase produces polyunsaturated fatty acids and is susceptible to oxidative stress induced by lipid peroxidation

Čipak, Ana; Hasslacher, Meinhard; Tehlivets, Oksana; Collinson, Emma J.; Živković, Morana; Matijević, Tanja; Wonisch, Willibald; Waeg, Georg; Dawes, Ian W.; Žarković, Neven; Kohlwein, Sepp D.

doi:10.1016/j.freeradbiomed.2005.10.039

Cited by 40 publications

(40 citation statements)

References 34 publications

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“…Previously, we have constructed a yeast strain endogenously producing PUFAs, mostly linoleic acid (C18:2, n-6 PUFA), and to the smaller extend hexadecadienoic acid (C16:2, n-4 PUFA), by the episomal expression of the D12-fatty acid desaturase from the plant Hevea brasiliensis [31]. Such conditional manipulation of yeast membrane opens a possibility to study metabolic changes and mechanisms behind the adaptation to PUFAs and LPO on this simplified model.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional and antioxidative responses to endogenous polyunsaturated fatty acid accumulation in yeast

et al. 2014

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Pathophysiology of polyunsaturated fatty acids (PUFAs) is associated with aberrant lipid and oxygen metabolism. In particular, under oxidative stress, PUFAs are prone to autocatalytic degradation via peroxidation, leading to formation of reactive aldehydes with numerous potentially harmful effects. However, the pathological and compensatory mechanisms induced by lipid peroxidation are very complex and not sufficiently understood. In our study, we have used yeast capable of endogenous PUFA synthesis in order to understand the effects triggered by PUFA accumulation on cellular physiology of a eukaryotic organism. The mechanisms induced by PUFA accumulation in S. cerevisiae expressing Hevea brasiliensis Δ12-fatty acid desaturase include down-regulation of components of electron transport chain in mitochondria as well as up-regulation of pentose-phosphate pathway and fatty acid β-oxidation at the transcriptional level. Interestingly, while no changes were observed at the transcriptional level, activities of two important enzymatic antioxidants, catalase and glutathione-S-transferase, were altered in response to PUFA accumulation. Increased intracellular glutathione levels further suggest an endogenous oxidative stress and activation of antioxidative defense mechanisms under conditions of PUFA accumulation. Finally, our data suggest that PUFA in cell membrane causes metabolic changes which in turn lead to adaptation to endogenous oxidative stress.

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Section: Introductionmentioning

confidence: 99%

Transcriptional and antioxidative responses to endogenous polyunsaturated fatty acid accumulation in yeast

et al. 2014

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“…In accordance with Guvenc et al (2010), different sugar source in nutrition media increased the fatty acid synthesis. Therefore, these circumstances has contributed to the thinking that H 2 O 2 has negative effect on fatty acid synthesis of genes (Kajiwara et al 1997;Cipak et al 2006;Ozsahin et al 2009). In our study, we discovered that vitamin synthesis was made from Saccharomyces cerevisiae: the vitamin rates were α-tocoferol, "γ-tocoferol, β-sitosterol cholesterol, stigmasterol ergesterol, D, and K 1,2.…”

Section: Resultsmentioning

confidence: 99%

“…Saccharomyces cerevisiae is important yeast that has been used for various studies (Kagan et al 2005;Comitini et al 2011). H 2 O 2 is a reactive oxygen species (ROS) in organism, being permanently produced intracellularly as a product of the metabolism in aerobic organisms and otherwise extracellularly during infection in specialist organisms (Lopes et al 2004;Cipak et al 2006;Folmer et al 2008). The consumption of H 2 O 2 by Saccharomyces cerevisiae is to change the synthesis of fatty acid and total protein in plasma membrane (Matias et al 2007;Folmer et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Anti-Oxidant effects of pomegranate juice on <i>Saccharomyces cerevisiae</i> cell growth

Aslan

Can

Boydak

2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Pomegranate juice has a number of positive effects on both human and animal subjects. Material and methods: Four groups were used in this study. i: Control group, ii: H 2 O 2 group, iii: Pomegranate juice (PJ) group and iv: PJ + H 2 O 2 group. Following the sterilization method for pomegranate juice (10%) and H 2 O 2 (6% v/v), Saccharomyces cerevisiae cultures were added and the cultivation incubated at 35°C for 72 hours. Fatty acids and vitamin concentrations were measured using HPLC and GC and the total protein bands profile were determined by SDS-PAGE. Results: According to our results statistically significant differences have been determined among the study groups in terms of fatty acids and vitamin (p<0,05). Fatty acid synthesis, vitamin control and cell density increased in groups to which PJ was given in comparison with the control group (p<0,05). Pomegranate juice increased vitamins, fatty acids and total protein expression in Saccharomyces cerevisiae in comparison with the control. Conclusion: Pomegranate juice has a positive effect on fatty acid, vitamin and protein synthesis by Saccharomyces cerevisiae. Accordingly, we believe that it has significantly decreased oxidative damage thereby making a positive impact on yeast development.

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“…Complex molecules such as hydrocortisone, a 27-carbon molecule, are synthesized in S. cereVisiae from ethanol (2-carbon molecule) by the cloning of nine cDNAs and the deletion of four host genes (45,46). Similar attempts to make taxol intermediates, taxadien-5d-aceoxy-10 -ol, and long-chain polyunsaturated fatty acids in yeast by assembling heterologous pathways have been reported, although with moderate success in improving rates and yields of the desired product (titers are in mg/L) (47)(48)(49)(50)(51). Production of plant-specific polyphenols such as naringenin and resveratrol in E. coli and yeast has been successful through engineering of heterologous pathways (52)(53)(54)(55)(56)(57).…”

Section: Metabolic Engineering Tools and Approachesmentioning

confidence: 99%

Engineering Complex Phenotypes in Industrial Strains

Patnaik

2008

Biotechnol. Prog.

101

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The global demand is rising for greener manufacturing processes that are cost-competitive and available in a timely manner. This has led to the development of a series of new tools and integrative platforms enabling rapid engineering of complex phenotypes in industrial microbes. By blending "old classical methods" of strain isolation with "newer approaches" of cell engineering, researchers are demonstrating the ability to stack multiple complex phenotypes in industrial hosts with some level of certainty. Newer tools for dissecting the genotype-phenotype correlation include association analysis (Precision Engineering), multiSCale Analysis of Library Enrichment (SCALE) in competition experiments, whole-genome transcriptional analysis, and proteomics and metabolomics technology. These newer and older tools of metabolic engineering and synthetic biology when combined with recent whole cell engineering approaches like whole genome shuffling, global transciptome machinery engineering, and directed evolutionary engineering, provide a powerful platform for engineering complex phenotypes in industrial strains. This review attempts to highlight and compare these newer tools and approaches with traditional strain isolation procedures as it applies to genome engineering with examples taken from literature.

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Saccharomyces cerevisiae strain expressing a plant fatty acid desaturase produces polyunsaturated fatty acids and is susceptible to oxidative stress induced by lipid peroxidation

Cited by 40 publications

References 34 publications

Transcriptional and antioxidative responses to endogenous polyunsaturated fatty acid accumulation in yeast

Transcriptional and antioxidative responses to endogenous polyunsaturated fatty acid accumulation in yeast

Anti-Oxidant effects of pomegranate juice on <i>Saccharomyces cerevisiae</i> cell growth

Engineering Complex Phenotypes in Industrial Strains

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