Saccharomyces cerevisiae Mer3 Helicase Stimulates 3′–5′ Heteroduplex Extension by Rad51

Mazina, Olga M.; Mazin, Alexander V.; Nakagawa, Takuro; Kolodner, Richard D.; Kowalczykowski, Stephen C.

doi:10.1016/s0092-8674(04)00294-6

Cited by 106 publications

(92 citation statements)

References 72 publications

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“…Thus, these results suggest that RPA2c is required for the majority or all of class I COs but may not have an effect on class II COs. MER3 encodes a DNA helicase containing a DEXH box domain that unwinds duplex DNA in the 39 to 59 direction (Nakagawa et al, 2001). It is postulated that MER3 could stabilize nascent interactions via DNA heteroduplex extension to promote capture of the second DNA end, leading to the formation of the double Holliday junctions (Mazina et al, 2004). In our study, the majority of MER3 foci colocalized with RPA2c, and wild-type levels of MER3 foci appear in the rpa2c background.…”

Section: Role Of Rpa2c In Meiotic Recombinationsupporting

confidence: 49%

Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

Chang

Xin

et al. 2013

The Plant Cell

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Replication protein A (RPA) is a conserved heterotrimeric protein complex comprising RPA1, RPA2, and RPA3 subunits involved in multiple DNA metabolism pathways attributable to its single-stranded DNA binding property. Unlike other species possessing a single RPA2 gene, rice (Oryza sativa) possesses three RPA2 paralogs, but their functions remain unclear. In this study, we identified RPA2c, a rice gene preferentially expressed during meiosis. A T-DNA insertional mutant (rpa2c) exhibited reduced bivalent formation, leading to chromosome nondisjunction. In rpa2c, chiasma frequency is reduced by ;78% compared with the wild type and is accompanied by loss of the obligate chiasma. The residual ;22% chiasmata fit a Poisson distribution, suggesting loss of crossover control. RPA2c colocalized with the meiotic cohesion subunit REC8 and the axis-associated protein PAIR2. Localization of REC8 was necessary for loading of RPA2c to the chromosomes. In addition, RPA2c partially colocalized with MER3 during late leptotene, thus indicating that RPA2c is required for class I crossover formation at a late stage of homologous recombination. Furthermore, we identified RPA1c, an RPA1 subunit with nearly overlapping distribution to RPA2c, required for ;79% of chiasmata formation. Our results demonstrate that an RPA complex comprising RPA2c and RPA1c is required to promote meiotic crossovers in rice.

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Section: Role Of Rpa2c In Meiotic Recombinationsupporting

confidence: 49%

Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

Chang

Xin

et al. 2013

The Plant Cell

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“…The considerable sequence similarity (73%) between the helicase domain of Mus301 and that of Hs HEL308 raises the possibility that mus301 possesses a DNA-unwinding activity with 39-59 polarity. In support of our model, budding yeast mer3 encodes an ATP-dependent DNA helicase that unwinds dsDNA with a 39-59 polarity and that stimulates 39-59 heteroduplex extension by Rad51 in crossover recombination (Mazina et al 2004). Finally, okra, a gene required for the repair of DSB after P-element excision and for DNA repair during oogenesis (Kooistra et al 1997;Ghabrial et al 1998;Kooistra et al 1999;Romeijn et al 2005), is the Drosophila homolog of yeast Rad54, a SWI2/SNF2 chromatin-remodeling dsDNAdependent ATPase that binds Rad51 directly and that stimulates DSB repair in both meiotic and mitotic cells (Mazin et al 2000;Krogh and Symington 2004).…”

Section: Discussionmentioning

confidence: 52%

Drosophila mus301/spindle-C Encodes a Helicase With an Essential Role in Double-Strand DNA Break Repair and Meiotic Progression

2006

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mus301 was identified independently in two genetic screens, one for mutants hypersensitive to chemical mutagens and another for maternal mutants with eggshell defects. mus301 is required for the proper specification of the oocyte and for progression through meiosis in the Drosophila ovary. We have cloned mus301 and show that it is a member of the Mus308 subfamily of ATP-dependent helicases and the closest homolog of human and mouse HEL308. Functional analyses demonstrate that Mus301 is involved in chromosome segregation in meiosis and in the repair of double-strand-DNA breaks in both meiotic and mitotic cells. Most of the oogenesis defects of mus301 mutants are suppressed by mutants in the checkpoint kinase Mei41 and in MeiW68, the Spo11 homolog that is thought to generate the dsDNA breaks that initiate recombination, indicating that these phenotypes are caused by activation of the DNA damage checkpoint in response to unrepaired Mei-W68-induced dsDNA breaks. However, neither mei-W68 nor mei-41 rescue the defects in oocyte specification of mus301 mutants, suggesting that this helicase has another function in oocyte selection that is independent from its role in meiotic recombination.

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“…The SDSA mechanism appears to be more common than the DHJ in the mitotic germ cells of wild-type Drosophila (Nassif et al 1994;Rong and Golic 2003). In meiotic cells, the choice between these two pathways is thought to determine whether a given DSB will result in gene conversion or crossover (Allers and Lichten 2001;Hunter and Kleckner 2001;Borner et al 2004;Mazina et al 2004). …”

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confidence: 99%

Differential Usage of Alternative Pathways of Double-Strand Break Repair in Drosophila

2006

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Double-strand DNA breaks can be repaired by any of several alternative mechanisms that differ greatly in the nature of the final repaired products. We used a reporter construct, designated ''Repair reporter 3'' (Rr3), to measure the relative usage of these pathways in Drosophila germ cells. The method works by creating a double-strand break at a specific location such that expression of the red fluorescent protein, DsRed, in the next generation can be used to infer the frequency at which each pathway was used. A key feature of this approach is that most data come from phenotypic scoring, thus allowing large sample sizes and considerable precision in measurements. Specifically, we measured the proportion of breaks repaired by (1) conversion repair, (2) nonhomologous end joining (NHEJ), or (3) single-strand annealing (SSA). For conversion repair, the frequency of mitotic crossing over in the germ line indicates the relative prevalence of repair by double Holliday junction (DHJ) formation vs. the synthesis-dependent strand annealing (SDSA) pathway. We used this method to show that breaks occurring early in germ-line development were much more frequently repaired via single-strand annealing and much less likely to be repaired by end joining compared with identical breaks occurring later in development. Conversion repair was relatively rare when breaks were made either very early or very late in development, but was much more frequent in between. Significantly, the changes in relative usage occurred in a compensatory fashion, such that an increase in one pathway was accompanied by decreases in others. This negative correlation is interpreted to mean that the pathways for double-strand break repair compete with each other to handle a given breakage event.

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Saccharomyces cerevisiae Mer3 Helicase Stimulates 3′–5′ Heteroduplex Extension by Rad51

Cited by 106 publications

References 72 publications

Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

Drosophila mus301/spindle-C Encodes a Helicase With an Essential Role in Double-Strand DNA Break Repair and Meiotic Progression

Differential Usage of Alternative Pathways of Double-Strand Break Repair in Drosophila

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