Saccharomyces cerevisiae killer toxins: synthesis, mechanisms of action and practical use

Викторовна, Самбук Елена; Михайлович, Музаев Дмитрий; Михайлович, Румянцев Андрей; Владимировна, Падкина Марина

doi:10.17816/ecogen17359-73

Cited by 2 publications

(1 citation statement)

References 75 publications

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“…2 расы из Коллекции микроорганизмов виноделия «Магарач» и активные сухие дрожжи LALVIN QA-23 (Дан стар Фермент АГ, Швейцария) (табл. 1), в том числе S-чувствительные и с киллер-фактором, который способствует доминированию данной расы при брожении [10,11].…”

Section: объекты и методы исследованияunclassified

Perspective Yeast Races for Young Sparkling Wines with a Muscat Aroma

Lutkov

Yermolin

Zadorozhnaya

et al. 2021

Food Processing: Techniques and Technology

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Introduction. In order to solve the growing demand for Muscat sparkling wines, some of the Muscat grapes used for the production of liqueur wines should be used as a raw material for sparkling wines. The technology of preparing young sparkling wines has a shorter production cycle. Science-based selection of the yeast race makes it possible to obtain young sparkling wines with a clear and bright Muscat aroma. Study objects and method. The research featured young sparkling wines from White Muscat grapes, produced using different yeasts races. The research involved both standard and modified methods of enochemical analysis. Results and discussion. The LALVIN QA-23 and Odesskiy Cherniy-SD-13 yeast races demonstrated a more active fermentation of Muscat white wort. The Sevastopolskaya 23 sample had in a slower fermentation, which promoted a greater accumulation of bound forms of carbon dioxide (16%). It contained the smallest amount of residual sugars (2 g/dm3) and thriable acids (6 g/dm3) and the largest amount of polyphenols (103 mg/dm3) and amine nitrogen (91 mg/dm3). Two samples accumulated a sufficient amount of free terpenes: 1.16 mg/dm3 for LALVIN QA-23 and 1.13 mg/dm3 for Sevastopolskaya 23. The degustation scores were quite high: LALVIN QA-23 received 9.03 points and Odesskiy Cherniy-SD-13 – 9.02 points, while Sevastopolskaya 23 scored 9.00 points. The LALVIN QA-23 sample proved to have the most pronounced varietal aroma, whereas the Odesskiy Cherniy-SD-13 sample possessed a more complex aromatic complex. Conclusion. The LALVIN QA-23 and Sevastopolskaya 23 yeast races were found suitable for bottle champagnization of young Muscat sparkling wines. They accumulated a sufficient amount of terpene alcohols. The samples had a clear Muscat aroma, harmonious taste, and good typical properties. The new method makes it possible to produce high quality sparkling wines in the harvest year.

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Section: объекты и методы исследованияunclassified

Perspective Yeast Races for Young Sparkling Wines with a Muscat Aroma

Lutkov

Yermolin

Zadorozhnaya

et al. 2021

Food Processing: Techniques and Technology

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Selective system based on fragments of the M1 virus for the yeast Saccharomyces cerevisiae transformation

Muzaev

Михайлович²,

Rumyantsev

et al. 2020

Ecological genetics

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Background. A selective system based on the M1 virus of the yeast Saccharomyces cerevisiae was proposed. Methods. To create a recipient strain, a DNA fragment encoding the killer toxin of the M1 virus under the control of the regulated promoter of the GAL1 gene was inserted into the genome of S. cerevisiae strains Y-1236 and Y-2177. Results. Integration of such expression cassette leads to the conditional lethality - resulting strains die on a medium with galactose when killer toxin synthesis occurs. A linear DNA fragment containing the gene of interest flanked by sequences homologous to the promoter of the GAL1 gene and the termination region of the CYC1 gene is used to transform the obtained strains. During transformation due to homologous recombination, the sequence encoding the killer toxin is cleaved and the transformants grow on a medium with galactose. Conclusion. The proposed selective system combines the main advantages of other systems: the use of simple media, without the need to add expensive antibiotics, and a simplified technique for constructing expression cassettes and selecting transformants.

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Saccharomyces cerevisiae killer toxins: synthesis, mechanisms of action and practical use

Cited by 2 publications

References 75 publications

Perspective Yeast Races for Young Sparkling Wines with a Muscat Aroma

Perspective Yeast Races for Young Sparkling Wines with a Muscat Aroma

Selective system based on fragments of the M1 virus for the yeast Saccharomyces cerevisiae transformation

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