Saccharomyces cerevisiae Gpi2, an accessory subunit of the enzyme catalyzing the first step of glycosylphosphatidylinositol (GPI) anchor biosynthesis, selectively complements some of the functions of its homolog in Candida albicans

Yadav, Anshuman; Singh, Sneh Lata; Yadav, Bhawna; Komath, Sneha Sudha

doi:10.1007/s10719-014-9536-8

Cited by 9 publications

(9 citation statements)

References 16 publications

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“…Amplified mutants WT + and WT − were inserted in CIp10 vector between Xho I and Mlu I restriction sites respectively. The mutants were transformed into CIA4 − using lithium acetate (LiAc) method [28]. The colonies were selected on SD Ura minimal medium.…”

Section: Methodsmentioning

confidence: 99%

Alteramide B is a microtubule antagonist of inhibiting Candida albicans

Ding

et al. 2016

Biochimica et Biophysica Acta (BBA) - General Subjects

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Background Alteramide B (ATB), isolated from Lysobacter enzymogenes C3, was a new polycyclic tetramate macrolactam (PTM). ATB exhibited potent inhibitory activity against several yeasts, particularly Candida albicans SC5314, but its antifungal mechanism is unknown. Methods The structure of ATB was established by extensive spectroscopic analyses, including high-resolution mass spectrometry, 1D- and 2D-NMR, and CD spectra. Flow cytometry, fluorescence microscope, transmission electron microscope, molecular modeling, overexpression and site-directed mutation studies were employed to delineate the anti-candida molecular mechanism of ATB. Results ATB induced apoptosis in C. albicans through inducing reactive oxygen species (ROS) production by disrupting microtubules. Molecular dynamics studies revealed the binding patterns of ATB to the β-tubulin subunit. Overexpression of the wild type and site-directed mutants of the β-tubulin gene (TUBB) changed the sensitivity of C. albicans to ATB, confirming the binding of ATB to β-tubulin, and indicating that the binding sites are L215, L217, L273, L274 and R282. In vivo, ATB significantly improved the survival of the candidiasis mice and reduced fungal burden. Conclusion The molecular mechanism underlying the ATB-induced apoptosis in C. albicans is through inhibiting tubulin polymerization that leads to cell cycle arrest at the G2/M phase. The identification of ATB and the study of its activity provide novel mechanistic insights into the mode of action of PTMs against the human pathogen. General significance This study shows that ATB is a new microtubule inhibitor and a promising anti-Candida lead compound. The results also support β-tubulin as a potential target for anti-Candida drug discovery.

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Section: Methodsmentioning

confidence: 99%

Alteramide B is a microtubule antagonist of inhibiting Candida albicans

Ding

et al. 2016

Biochimica et Biophysica Acta (BBA) - General Subjects

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“…To visualize CaGpi2-mRFP expression levels in the C. albicans strains, because protein expression levels for GPI-GnT subunits are low (15,46), immunofluorescence experiments with rabbit anti-mRFP antibodies (Abcam) and TRITC-labeled goat anti-rabbit IgG antibodies (GeNei) were performed. The fluorescence intensity in the cells was quantified using Olympus Fluoview software.…”

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confidence: 99%

Ras signaling activates glycosylphosphatidylinositol (GPI) anchor biosynthesis via the GPI–N-acetylglucosaminyltransferase (GPI–GnT) in Candida albicans

Jain

Sethi

Pratyusha

et al. 2018

Journal of Biological Chemistry

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The ability of to switch between yeast to hyphal form is a property that is primarily associated with the invasion and virulence of this human pathogenic fungus. Several glycosylphosphatidylinositol (GPI)-anchored proteins are expressed only during hyphal morphogenesis. One of the major pathways that controls hyphal morphogenesis is the Ras-signaling pathway. We examine the cross-talk between GPI anchor biosynthesis and Ras signaling in We show that the first step of GPI biosynthesis is activated by Ras in This is diametrically opposite to what is reported in Of the two Ras proteins, CaRas1 alone activates GPI-GnT activity; activity is further stimulated by constitutively activated CaRas1. CaRas1 localized to the cytoplasm or endoplasmic reticulum (ER) is sufficient for GPI-GnT activation. Of the six subunits of the GPI--acetylglucosaminyltransferase (GPI-GnT) that catalyze the first step of GPI biosynthesis, CaGpi2 is the key player involved in activating Ras signaling and hyphal morphogenesis. Activation of Ras signaling is independent of the catalytic competence of GPI-GnT. This too is unlike what is observed in where multiple subunits were identified as inhibiting Ras2. Fluorescence resonance energy transfer (FRET) studies indicate a specific physical interaction between CaRas1 and CaGpi2 in the ER, which would explain the ability of CaRas1 to activate GPI-GnT. CaGpi2, in turn, promotes activation of the Ras-signaling pathway and hyphal morphogenesis. The mutant is also more susceptible to macrophage-mediated killing, and macrophage cells show better survival when co-cultured with .

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“…In case of application as green manure Azolla collected directly from ponds is applied in the field. Azolla thus incorporated decomposes within about 8-10 days and releases the fixed nitrogen (Yadav et al 2014). To supply organic matter from Azolla and save when the production of Azolla is abundant then Azolla needs to be dried or composted.…”

Section: Role Of Azollamentioning

confidence: 99%

Nutrient Management Technologies and the Role of Organic Matrix-Based Slow-Release Biofertilizers for Agricultural Sustainability: A Review

Chaudhary

Neeraj

Siddiqui

et al. 2020

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Natural soil containing nutrients (like nitrogen, phosphorus, calcium and potassium) allows plants to grow. The deficiency of nutrients in soil reduced the growth and development of plants. When the nutrient level is too low, the plant cannot function properly and then fertilizers provide sufficient nutrients to plants. Nowadays the farmer applied various kinds of synthetic and organic and some special class of microbial fertilizers for more production of agricultural crops. Excess use of chemical fertilizers caused nutrient leaching problems and also pollutes soil, water and air environment. However, chemical fertilizers are expensive, non-eco-friendly, cause eutrophication, reduce organic matter and microbial activity in the soil and are hazardous to health. Therefore, Slow-release biofertilizers are also produced by the technical intermediations that breakdown the nutrients and make them available to the plants for a longer duration. These fertilizers play an important role in improving the growth and development of plants, thereby mitigating environmental pollution and helping in sustainable agriculture. The efficacy of slow-release biofertilizers can be enhanced plant growth and productivity and manage the nutrient leaching from soil and also control water pollution.

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Saccharomyces cerevisiae Gpi2, an accessory subunit of the enzyme catalyzing the first step of glycosylphosphatidylinositol (GPI) anchor biosynthesis, selectively complements some of the functions of its homolog in Candida albicans

Cited by 9 publications

References 16 publications

Alteramide B is a microtubule antagonist of inhibiting Candida albicans

Alteramide B is a microtubule antagonist of inhibiting Candida albicans

Ras signaling activates glycosylphosphatidylinositol (GPI) anchor biosynthesis via the GPI–N-acetylglucosaminyltransferase (GPI–GnT) in Candida albicans

Nutrient Management Technologies and the Role of Organic Matrix-Based Slow-Release Biofertilizers for Agricultural Sustainability: A Review

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