Saccharomyces Cerevisiae as a Tool to Evaluate the Effects of Herbicides on Eukaryotic Life

Synergistic toxicity between glyphosate and 2,4-dinitrophenol on budding yeast is not due to H₂O₂-mediated oxidative stress

“…We tested if the toxic synergetic effect was a consequence of increased oxidative stress. Glyphosate was found to induce oxidative stress in Arabidopsis thaliana plants and in yeast [18] [14]. A mild mitochondrial decoupling activity observed with low concentrations of DNP (1 nM) is supposed to decrease the mitochondrion-generated oxidative stress and to increase yeast life span [9] but high concentrations could have the opposite effect as suggested with millimolar range concentrations on Hordeum vulgare plants [19].…”

“…Among them, Braconi and coll. showed that, in its commercial formulation Silglif ™ , glyphosate promotes significant oxidative stress in S. cerevisiae [13] [14]. We thus investigated the involvement of the oxidative stress in the toxic interaction between glyphosate and DNP using the double knockout ctt1Δ/cta1Δ deficient in the H 2 O 2 -detoxifying catalase activity.…”