Saccharomyces boulardii improves humoral immune response to DNA vaccines against leptospirosis

Silveira, Marcelle Moura; Conceição, Fabrício Rochedo; Mendonça, Marcelo; Moreira, Gustavo Marçal Schmidt Garcia; Cunha, C. E.; Conrad, Neida Lucia; Oliveira, Patrícia Diaz de; Hartwig, Daiane Drawanz; Leon, Priscila Marques Moura de; Moreira, Ângela Nunes

doi:10.1099/jmm.0.000414

Cited by 10 publications

(8 citation statements)

References 43 publications

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“…Nevertheless, this study demonstrated the feasibility of using a whole organism as a carrier of leptospiral proteins expressed at its surface to induce an immune response against leptospires after oral administration. The yeast Saccharomyces boulardi, administered in food pellets, and generally recognized as a safe (GRAS) organism, was recently shown to enhance the antibody and cytokine responses in Balb/C mice after 3 injections of DNA vaccines expressing LigA or LigB fragments [87]. However, no challenge was done with L. interrogans and it is not known whether S. boulardi improves protection against live bacteria.…”

Section: Oral Vaccinesmentioning

confidence: 99%

Recent findings related to immune responses against leptospirosis and novel strategies to prevent infection

Vernel-Pauillac

Werts

2018

Microbes and Infection

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What are the new approaches and emerging ideas to prevent leptospirosis, a neglected bacterial re-emerging zoonotic disease? How do Leptospira interrogans escape the host defenses? We aim here to review and discuss the most recent literature that provides some answers to these questions, in particular data related to a better understanding of adaptive and innate immunity towards leptospires, and design of vaccines. This is an opinion paper, not a comprehensive review. We will try to highlight the new strategies and technologies boosting the search for drugs and vaccines. We will also address the bottlenecks and difficulties impairing the search for efficient vaccines and the many gaps in our knowledge of immunity against leptospirosis. Finally, we aim to delineate how Leptospira spp. escape the innate immune responses of Toll-Like receptors (TLR) and Nod-Like receptors (NLR). The rational use of TLR and NLR agonists as adjuvants could be key to design future vaccines against pathogenic leptospires.

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Section: Oral Vaccinesmentioning

confidence: 99%

Recent findings related to immune responses against leptospirosis and novel strategies to prevent infection

Vernel-Pauillac

Werts

2018

Microbes and Infection

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“…Indirect ELISA using sera before and after immunizations was used to evaluate the IgG antibody levels as previously described [15]. Briefly, 96-well polystyrene microplates (CralPlast) were coated with 200 ng/well of rLigAni or rLigBrep diluted in carbonate-bicarbonate buffer 0.05 M (pH 9.6).…”

Section: Methodsmentioning

confidence: 99%

Biopolymer Xanthan: A New Adjuvant for DNA Vaccines

Silveira

Conceição

Mendonça

et al. 2020

Braz. arch. biol. technol.

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DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan.

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“…Another study investigated the immunomodulatory effects of Saccharomyces boulardii , a probiotic known for enhancing anti-inflammatory cytokine production and immune cell activation ( Silveira et al, 2017 ). In combination with DNA vaccines encoding leptospiral protein fragments, S. boulardii significantly increased antibody titres and upregulated IL-10 expression, particularly with pTARGET/ligBrep vaccination.…”

Section: Role Of Probiotics In the Treatment Of Leptospirosismentioning

confidence: 99%

Current treatment options for leptospirosis: a mini-review

Petakh,

Behzadi,

Oksenych

et al. 2024

Front. Microbiol.

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Leptospirosis, one of the most common global zoonotic infections, significantly impacts global human health, infecting more than a million people and causing approximately 60,000 deaths annually. This mini-review explores effective treatment strategies for leptospirosis, considering its epidemiology, clinical manifestations, and current therapeutic approaches. Emphasis is placed on antibiotic therapy, including recommendations for mild and severe cases, as well as the role of probiotics in modulating the gut microbiota. Furthermore, novel treatment options, such as bacteriophages and newly synthesized/natural compounds, are discussed, and the findings are expected to provide insights into promising approaches for combating leptospirosis.

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Saccharomyces boulardii improves humoral immune response to DNA vaccines against leptospirosis

Cited by 10 publications

References 43 publications

Recent findings related to immune responses against leptospirosis and novel strategies to prevent infection

Recent findings related to immune responses against leptospirosis and novel strategies to prevent infection

Biopolymer Xanthan: A New Adjuvant for DNA Vaccines

Current treatment options for leptospirosis: a mini-review

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