Saccharomyces boulardii attenuates inflammatory response induced by Clostridium perfringens via TLR4/TLR15-MyD8 pathway in HD11 avian macrophages

Wang, Baikui; Hussain, Altaf; Zhou, Yuguang; Zeng, Zihan; Wang, Qi; Zou, Peng; Gong, Li; Zhao, Pengwei; Li, Weifen

doi:10.1016/j.psj.2020.07.045

Cited by 21 publications

(21 citation statements)

References 55 publications

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“…However, when cells were pre-incubated with L. plantarum Lac16, the C. perfringens infection-associated increase in LDH release was effectively alleviated. Similar protective results have been reported [48][49][50][51].…”

Section: Discussionsupporting

confidence: 91%

In vitro protective effects of Lactobacillus plantarum Lac16 on Clostridium perfringens infection-associated intestinal injury in IPEC-J2 cells

Zhou

Wang

et al. 2021

Preprint

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Background: Clostridium perfringens causes intestinal injury through overgrowth and secretion of multiple toxins, leading to diarrhea and necrotic enteritis in animals, such as pigs. Lactobacillus plantarum (L. plantarum) Lac16 has been reported to protect broilers against C. perfringens infection. This study aimed at investigating the protective effects of Lactobacillus plantarum Lac16 on C. perfringens infection-associated intestinal injury in intestinal porcine epithelial cell line (IPEC-J2). Results: The results showed that L. plantarum Lac16 significantly inhibit the growth and biofilm formation of C. perfringens (P < 0.001). In the co-culture system, L. plantarum Lac16 significantly suppressed colony forming units (CFU) of C. perfringens (P < 0.05), which was accompanied by a decrease in pH levels (P < 0.01). Moreover, L. plantarum Lac16 significantly elevated the mRNA expression levels of host defense peptides (HDPs) in IPEC-J2 cells (P < 0.05), decreased C. perfringens-induced cellular cytotoxicity (P < 0.01) and adhesion to cells (P < 0.05). At the same time, L. plantarum Lac16 significantly attenuated C. perfringens-induced damage to intestinal barrier integrity and the decrease in claudin-1 (P < 0.01) as well as zona occludens 1 (ZO-1) expressions. Preincubation with L. plantarum Lac16 significantly suppressed mRNA expression levels of pattern recognition receptors (PRRs) (Toll-like receptor (TLR) 1, TLR2, nucleotide-binding oligomerization domain (NOD) 1) in C. perfringens-challenged IPEC-J2 cells (P < 0.01). C. perfringens significantly elevated the phosphorylation of p38 mitogen-activated protein kinase (MAPK), JNK, and p65 nuclear factor-κB (NF-κB) (P < 0.05) while L. plantarum Lac16 pre-incubation effectively inhibited phosphorylation of p65 (P < 0.001). Furthermore, L. plantarum Lac16 significantly suppressed C. perfringens induced gene expressions of proinflammatory cytokines (interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor-α (TNF-α)) (P < 0.05). Conclusions: Collectively, probiotic L. plantarum Lac16 exerts protective effects against C. perfringens infection-associated intestinal injury in IPEC-J2 cells.

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Section: Discussionsupporting

confidence: 91%

In vitro protective effects of Lactobacillus plantarum Lac16 on Clostridium perfringens infection-associated intestinal injury in IPEC-J2 cells

Zhou

Wang

et al. 2021

Preprint

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“…Generally, the composition of intestinal microbiota in birds is relatively stable and resilient over time ( Clemente et al., 2018 ). However, various environmental factors, such as feed, stresses, viruses, and drugs, especially antibiotics, may lead to intestinal dysbacteriosis and immune dysregulation in birds, which consequently compromises growth performance, bird welfare and may induce the occurrence of intestinal necrotic diseases ( Isaac et al., 2017 ; Kernbauer et al., 2014 ; Prescott et al., 2016 ; Wang et al., 2020 ). Therefore, a balanced intestinal microbiota is crucial for the improvement of overall performance and quality of poultry products.…”

Section: Introductionmentioning

confidence: 99%

Probiotic Paenibacillus polymyxa 10 and Lactobacillus plantarum 16 enhance growth performance of broilers by improving the intestinal health

Wang¹,

Gong²,

Zhou³

et al. 2021

Animal Nutrition

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With the ever-growing strict prohibitions on antibiotic growth promoters (AGP) in animal production, in-feed probiotics are becoming attractive alternatives to antibiotics in the poultry industry. To investigate the effects of Paenibacillus polymyxa 10 and Lactobacillus plantarum 16 on the growth performance and intestinal health of broilers, 540 male Cobb 500 broilers of 1 d old were randomly divided into 3 groups with 6 replicates per group and 30 chicks per replicate. Broilers were fed with either a basal diet or basal diets supplemented with 1 × 10 8 colony-forming units (CFU)/kg P. polymyxa 10 (BSC10) or L. plantarum 16 (Lac16) for 42 d. Results showed that Lac16 treatment improved ( P < 0.05) the growth performance (body weight and feed conversion) of broilers at the starter phase, while BSC10 treatment slightly improved ( P > 0.05) the growth performance of the starter phase broilers. The increased villus height ( P < 0.05) at d 14, 21 and 42 and villus height to crypt depth ratio ( P < 0.05) at d 14 and 21 were observed in the ileum of the 2 probiotic groups. Besides, transmission electron microscopy results showed that the 2 probiotics enhanced the intestinal epithelial barrier. Both probiotic treatments up-regulated ( P < 0.05) the mRNA expression of fatty acid binding protein 1 ( FABP1 ) and sodium-dependent glucose transporters-1 ( SGLT-1 ) in the ileal mucosa of broilers at d 21. In addition, BSC10 and Lac16 treatments significantly ( P < 0.05) increased the relative abundance of short-chain fatty acids-producing bacteria, such as Butyricicoccus pullicaecorum , Faecalibacterium prausnitzii , Lachnospira and Coprococcu , and significantly ( P < 0.05) decreased the relative abundance of enteric pathogens ( Escherichia coli , Bacteroides fragilis and Shigella sonnei ). Furthermore, the 2 probiotic treatments also increased the positive connection among the intestinal microbes and the carbohydrate metabolism-related pathways of the intestinal bacteria ( P < 0.05), with decreasing ( P < 0.05) nucleotides biosynthesis-related pathways of the intestinal bacteria. Overall, these results suggest that the 2 probiotics, especially Lac16, have a potential beneficial effect on the growth performance and intestinal health of starter phase broilers.

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“…The result was consistent with previous studies that subclinical necrotic enteritis infection impaired broiler growth performance with no mortality ( 36 , 37 ). Our previous study reported that Saccharomyces boulardii attenuates C. perfringens -induced inflammatory response via the TLR4/TLR15-MyD88-signaling pathway in HD11 avian macrophages ( 23 ). Bacillus amyloliquefaciens could alleviate necrotic enteritis-induced undesirable effects by modulation of genes related to gut integrity, apoptosis, and immunity, hence further improve performance ( 38 ).…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have proved that L. plantarum exerts antibacterial activities by secreting lactic acid (18) and plantaricin (19,20), and P. polymyxa exerts antibacterial activities by secreting polymyxin and lantibiotic (21,22). Although numerous studies have demonstrated that probiotics, such as lactobacillus, bacillus, and yeast, could alleviate or abolish C. perfringens infection-induced necrotic enteritis (23)(24)(25)(26), the involved interactive roles of intestinal microbiota, microbial metabolic functions, and short-chain fatty acids (SCFAs) under C. perfringens infection-induced necrotic enteritis remain poorly understood. Our previous works found that two probiotics, Lactobacillus plantarum (Lac16) and Paenibacillus polymyxa (BSC10), had in vitro anti-C. perfringens activities and Lac16 protected-Caenorhabditis elegans against C. perfringens infection (Supplementary Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Dietary Supplementation With Lactobacillus plantarum Ameliorates Compromise of Growth Performance by Modulating Short-Chain Fatty Acids and Intestinal Dysbiosis in Broilers Under Clostridium perfringens Challenge

Wang¹,

Zhou²,

Mao³

et al. 2021

Front. Nutr.

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Clostridium perfringens is an important zoonotic pathogen associated with food contamination and poisoning, gas gangrene, necrotizing enterocolitis or necrotic enteritis in humans and animals. Dysbacteriosis is supposedly associated with the development of C. perfringens infection induced necrotic enteritis, but the detailed relationship between intestinal health, microbiome, and C. perfringens infection-induced necrotic enteritis remains poorly understood. This research investigated the effect of probiotics on the growth performance and intestinal health of broilers, and the involved roles of intestinal microbiota and microbial metabolic functions under C. perfringens infection. Results showed that subclinical necrotic enteritis was successfully induced as evidenced by the significant lower body weight (BW), suppressed feed conversion ratio (FCR), decreased ileal villus height and mucosal barrier function, and increased ileal histopathological score and bursal weight index. Lactobacillus plantarum or Paenibacillus polymyxa significantly attenuated C. perfringens-induced compromise of growth performance (BW, FCR) and ileal mucosa damage as illustrated by the increased ileal villus height and villus/crypt ratio, the decreased ileal histopathological score and the enhanced ileal mucosal barrier function. L. plantarum also significantly alleviated C. perfringens-induced enlarged bursa of fabricius and the decreased levels of ileal total SCFAs, acetate, lactate, and butyrate. Furthermore, dietary L. plantarum improved C. perfringens infection-induced intestinal dysbiosis as evidenced by significantly enriched short-chain fatty acids-producing bacteria (Lachnospiraceae, Ruminococcaceae, Oscillospira, Faecalibacterium, Blautia), reduced drug-resistant bacteria (Bacteroides, Alistipes) and enteric pathogens (Escherichia coli, Bacteroides fragilis) and bacterial metabolic dysfunctions as illustrated by significantly increased bacterial fatty acid biosynthesis, decreased bacterial lipopolysaccharide biosynthesis, and antibiotic biosynthesis (streptomycin and vancomycin). Additionally, the BW and intestinal SCFAs were the principal factors affecting the bacterial communities and microbial metabolic functions. The above findings indicate that dietary with L. plantarum attenuates C. perfringens-induced compromise of growth performance and intestinal dysbiosis by increasing SCFAs and improving intestinal health in broilers.

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Saccharomyces boulardii attenuates inflammatory response induced by Clostridium perfringens via TLR4/TLR15-MyD8 pathway in HD11 avian macrophages

Cited by 21 publications

References 55 publications

In vitro protective effects of Lactobacillus plantarum Lac16 on Clostridium perfringens infection-associated intestinal injury in IPEC-J2 cells

In vitro protective effects of Lactobacillus plantarum Lac16 on Clostridium perfringens infection-associated intestinal injury in IPEC-J2 cells

Probiotic Paenibacillus polymyxa 10 and Lactobacillus plantarum 16 enhance growth performance of broilers by improving the intestinal health

Dietary Supplementation With Lactobacillus plantarum Ameliorates Compromise of Growth Performance by Modulating Short-Chain Fatty Acids and Intestinal Dysbiosis in Broilers Under Clostridium perfringens Challenge

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