Saccharification of ionic-liquid-pretreated sugar cane bagasse using Penicillium echinulatum enzymes

Aver, Kaliane Regalin; Scortegagna, Angélica; Fontana, Roselei Claudete; Camassola, Marli

doi:10.1016/j.jtice.2014.04.017

Cited by 16 publications

(8 citation statements)

References 46 publications

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“…By comparing the images obtained for steam-exploded elephant grass with those presented by (Aver et al, 2014), it seems that steam explosion promotes an extensive fragmentation of the biomass fibers while ionic liquids restrict their pretreatment action on the dissolution of biomass components such as plant polysaccharides.…”

Section: Scanning Electron Microscopymentioning

confidence: 97%

Ethanol production from sugars obtained during enzymatic hydrolysis of elephant grass (Pennisetum purpureum, Schum.) pretreated by steam explosion

Scholl

Menegol

Pitarelo

et al. 2015

Bioresource Technology

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In this work, steam explosion was used a pretreatment method to improve the conversion of elephant grass (Pennisetum purpureum) to cellulosic ethanol. This way, enzymatic hydrolysis of vaccum-drained and water-washed steam-treated substrates was carried out with Penicillium echinulatum enzymes while Saccharomyces cerevisiae CAT-1 was used for fermentation. After 48 h of hydrolysis, the highest yield of reducing sugars was obtained from vaccum-drained steam-treated substrates that were produced after 10 min at 200 °C (863.42 ± 62.52 mg/g). However, the highest glucose yield was derived from water-washed steam-treated substrates that were produced after 10 min at 190 °C (248.34 ± 6.27 mg/g) and 200 °C (246.00 ± 9.60 mg/g). Nevertheless, the highest ethanol production was obtained from water-washed steam-treated substrates that were produced after 6 min at 200 °C. These data revealed that water washing is a critical step for ethanol production from steam-treated elephant grass and that pretreatment generates a great deal of water soluble inhibitory compounds for hydrolysis and fermentation, which were partly characterized as part of this study.

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Section: Scanning Electron Microscopymentioning

confidence: 97%

Ethanol production from sugars obtained during enzymatic hydrolysis of elephant grass (Pennisetum purpureum, Schum.) pretreated by steam explosion

Scholl

Menegol

Pitarelo

et al. 2015

Bioresource Technology

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“…Pretreatment with ionic 1-ethyl-3-methylimidazolium acetate and 1-butyl-3-methylimidazolium is very effective in dissolving lignocellulosic materials, such as grasses (Barr et al, 2012), rice husk (Poornejad et al, 2013), cane sugar bagasse (Sant'Ana da Silva et al, 2011), maple (Lee et al, 2009), pine (Brandt et al, 2011) and eucalyptus (Uju et al, 2012). However, the removal of excess ionic liquid in the lignocellulosic biomass prior to the enzymatic hydrolysis is necessary in order to avoid a negative effect on the cellulase activity and the consequent reduction in final concentrations of total reducing sugars (Alvira et al, 2010;Aver et al, 2014). However, the main reason is the price of ionic liquids (ILs), even the commercial grades.…”

Section: Brazilian Journal Of Chemical Engineeringmentioning

confidence: 99%

ETHANOL PRODUCTION FROM SUGAR LIBERATED FROM Pinus SP. AND Eucalyptus SP. BIOMASS PRETREATED BY IONIC LIQUIDS

Tura

Montipó

Fontana

et al. 2018

Braz. J. Chem. Eng.

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Pretreatment of lignocellulosic biomass using ionic liquids (ILs) has been widely studied and is considered one of the most promising methods to obtain fermentable sugars. However, few data exist on the fermentation of reducing sugars (RS) obtained by enzymatic hydrolysis of biomass pretreated with ionic liquids for the production of ethanol. Therefore, this study evaluated the production of ethanol from sugars liberated from sawdust of Pinus sp. and Eucalyptus sp. pretreated with ionic liquid [C 4 mim][OAc] and [C 2 mim] [OAc], hydrolyzed with enzymes of Penicillium echinulatum employing Saccharomyces cerevisiae and Schizosaccharomyces pombe Y698 yeasts. The data indicate that when the biomass is pre-treated by [C 2 mim] [OAc] there is higher production of ethanol that, when treated by [C 4 mim][OAc] for both evaluated yeasts, even though the pretreatment with [C 2 mim] [OAc] caused the highest losses of cellulose and hemicellulose. Under the conditions analyzed, it is possible to produce approximately 40 and 47 L of ethanol per ton of Pinus sp. and Eucalyptus sp, respectively. In addition to contributing to knowledge about the physiology of the yeasts in the sugars liberated from biomass pretreated in presence of ionic liquid, this data is also relevant to the development of processes for the production of lignocellulosic ethanol.

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“…Chemical pretreatment is the most common and studied pretreatment method for the conversion of lignocellulosic biomass to bioethanol. Different chemicals including alkali, ionic liquids, organic solvents, oxidizing agents, and acids can be used [30]. Acid pretreatment is one of the most promising methods and has been extensively studied.…”

Section: Chemical Pretreatmentmentioning

confidence: 99%

“…Ozone, a strong oxidizing agent is very effective for the removal of lignin in lignocellulosic biomass. This type of chemical pretreatment is normally done at room temperature and results in Fuel Ethanol Production from Sugarcane no inhibitor formation [30]. Organic solvents such as methanol, ethanol, ethylene glycol, glycerol, acetic acid, formic acid, phenol, and dioxane are also very effective in extracting lignin and hemicellulose [29].…”

Section: Chemical Pretreatmentmentioning

confidence: 99%

Potential of Weed Biomass for Bioethanol Production

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Fuel Ethanol Production From Sugarcane

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Lignocellulosic biomass from weedy plants represents a potential alternative feedstock for economic production of bioethanol. Large numbers of weedy plant species are growing all over the world. Characteristics such as high dry matter yield, low water and nutrient requirements for growth, and cellulose contents make weedy plants very attractive as feedstock for bioethanol production. However, like other lignocellulosic feedstock, the complex structure presents resistance and recalcitrance to processes of conversion to bioethanol. Several weedy plants have been studied to determine their physical characteristics and suitability for bioethanol production. Different conversion techniques have been employed to increase monomer sugars and hence bioethanol yield. This chapter discusses processes and current research activities in bioconversion of weed biomass to bioethanol.

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Saccharification of ionic-liquid-pretreated sugar cane bagasse using Penicillium echinulatum enzymes

Cited by 16 publications

References 46 publications

Ethanol production from sugars obtained during enzymatic hydrolysis of elephant grass (Pennisetum purpureum, Schum.) pretreated by steam explosion

Ethanol production from sugars obtained during enzymatic hydrolysis of elephant grass (Pennisetum purpureum, Schum.) pretreated by steam explosion

ETHANOL PRODUCTION FROM SUGAR LIBERATED FROM Pinus SP. AND Eucalyptus SP. BIOMASS PRETREATED BY IONIC LIQUIDS

Potential of Weed Biomass for Bioethanol Production

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