Saccharides in straw hydrolysate decrease cell membrane damage by phenolics by inducing the formation of extracellular matrix in yeast

Oligosaccharides in straw hydrolysate could improve the production of single‐cell protein with Saccharomyces cerevisiae

“…However, at 3 h, high enzyme loading resulted in relatively strong inhibition of the consumption of glucose and the production of ethanol. This may have been due to the release of some phenolic compounds during the saccharification of the straw, which inhibited the growth of microorganisms 22 . After saccharification, the total phenol content in the hydrolysates was similar (Fig.…”

“…Glycogen plays an indispensable role in yeast metabolism 22,37 . Certain components in the hydrolysate can promote glycogen synthesis.…”

“…Rice straw was harvested from Shanghai, China. After drying in an oven at 60 °C for 4 h, the straw was ground with a grinder and milled with a ball miller 22 . Then it was passed through a 1 mm sieve.…”

“…The soluble polysaccharides and oligosaccharides (0.03 g samples) were hydrolyzed by 0.3 mL 72% H 2 SO 4 at 30 °C for 1 h. The concentration of H 2 SO 4 was then adjusted to 4% and the samples were placed in a high pressure sterilizer at 121 °C for 1 h. The pH was adjusted to 5–6, and high‐performance liquid chromatography (HPLC) was used to analyze the sugar content quantitatively. All experiments were repeated three times 22 …”

“…After drying in an oven at 60 °C for 4 h, the straw was ground with a grinder and milled with a ball miller. 22 Then it was passed through a 1 mm sieve. Coarse straw was obtained and kept for 12 h at 60 °C, and then it was milled with a ball miller at room temperature (SFM-1, Hefei, Anhui Province, China).…”