Sa1804 Dysregulation of WNT5A/ROR2 Signaling Pathway Characterizes the Progression of Barrett's-Associated Esophageal Adenocarcinoma

Lyros, Orestis; Moore, Tami; Medda, Rituparna; Nie, Lei; Otterson, Mary F.; Gockel, Ines; Lang, Hauke; Shaker, Reza; Rafiee, Parvaneh

doi:10.1016/s0016-5085(14)61073-8

Cited by 2 publications

(2 citation statements)

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“…These genes showed similar trends of expression to CTNNB1 during feather follicle morphogenesis. Wnt5A could activate β-catenin signaling in the presence of FZD4 [ 53 – 55 ] and suppress the canonical Wnt signaling pathway in the presence of ROR2 via a GSK3-independent pathway, which involves downregulation of β-catenin-induced reporter gene expression [ 56 – 59 ]. The results suggested that Wnt/β-catenin signaling at the transcriptome level was upregulated in the late stage of feather follicle morphogenesis.…”

Section: Discussionmentioning

confidence: 99%

Skin transcriptome reveals the dynamic changes in the Wnt pathway during integument morphogenesis of chick embryos

Gong

Wang

et al. 2018

PLoS ONE

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Avian species have a unique integument covered with feathers. Skin morphogenesis is a successive and complex process. To date, most studies have focused on a single developmental point or stage. Fewer studies have focused on whole transcriptomes based on the time-course of embryo integument development. To analyze the global changes in gene expression profiles, we sequenced the transcriptome of chicken embryo skin samples from day 6 to day 21 of incubation and identified 5830 differentially expressed genes (DEGs). Hierarchical clustering showed that E6 to E14 is the critical period of feather follicle morphogenesis. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the DEGs, two kinds of Wnt signaling pathways (a canonical pathway and a non-canonical pathway) changed during feather follicle and feather morphogenesis. The gene expression level of inhibitors and ligands related to the Wnt signaling pathway varied significantly during embryonic development. The results revealed a staggered phase relationship between the canonical pathway and the non-canonical pathway from E9 to E14. These analyses shed new light on the gene regulatory mechanism and provided fundamental data related to integument morphogenesis of chickens.

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Section: Discussionmentioning

confidence: 99%

Skin transcriptome reveals the dynamic changes in the Wnt pathway during integument morphogenesis of chick embryos

Gong

Wang

et al. 2018

PLoS ONE

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“…This is the first report to show that metformin inhibited ROR2 activation. ROR2 correlates with a poor prognosis in various cancer types (37)(38)(39), cell migration/ invasion and metastasis (40)(41)(42); therefore, it has potential as a novel drug target in cancer therapy.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic potential of the antidiabetic drug metformin in small bowel adenocarcinoma

Chiyo

Kato

Iwama

et al. 2017

International Journal of Oncology

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Small bowel adenocarcinoma (SBAC) accounts for 3% of all gastrointestinal tract tumors and approximately 0.5% of all cancer cases. Recent studies have indicated that the use of metformin, one of the most commonly prescribed antidiabetic drugs, is associated with a better prognosis for certain malignant diseases. However, there have been no reports on the effect of metformin in SBAC. In the present study, we evaluated the effect of metformin on human SBAC cell proliferation in vitro and in vivo and identified the microRNAs (miRNAs) associated with its antitumor effects. Metformin inhibited the proliferation of HuTu80 cells in a time- and dose-dependent manner. Importantly, metformin reduced the expression of cyclin D1, cyclin E, cyclin-dependent kinase 4, and phosphorylated retinoblastoma protein, which resulted in cell cycle arrest at the G0/G1 phase. This arrest was accompanied by activation of AMPKα and inhibition of mammalian target of rapamycin and p70s6k. Additionally, metformin reduced the levels of phosphorylated epidermal growth factor receptor and ROR2 as well as markedly altered miRNA expression in HuTu80 cells. Metformin also inhibited tumor growth in vivo in a xenograft mouse model. Our data suggest that metformin might have therapeutic potential in SBAC.

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Sa1804 Dysregulation of WNT5A/ROR2 Signaling Pathway Characterizes the Progression of Barrett's-Associated Esophageal Adenocarcinoma

Cited by 2 publications

References 0 publications

Skin transcriptome reveals the dynamic changes in the Wnt pathway during integument morphogenesis of chick embryos

Skin transcriptome reveals the dynamic changes in the Wnt pathway during integument morphogenesis of chick embryos

Therapeutic potential of the antidiabetic drug metformin in small bowel adenocarcinoma

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