S33 Alpha-1 Antitrypsin is a Marker of Increased Intestinal Permeability in Ulcerative Colitis and COVID-19 Infection

Abstract

Background: Alpha-1-antitrypsin (AAT) is a protein that is the main inhibitor of neutrophil elastase and is released during inflammatory processes to reduce the activity of proteolytic enzymes in areas of inflammation. An indicator in the diagnosis of diseases of the small and large intestine. AAT plays an important role in the pathogenesis and regulation of the inflammatory process in inflammatory bowel diseases, can be used as diagnostic and prognostic markers in patients with intestinal lesions. The aim of the study was to determine the level of a-1-antitrypsin in the blood serum and in the feces in patients of UC with and without COVID-19 to assess the degree of inflammation activity and permeability in the intestine. Methods: Forty-two patients of UC with exacerbation of the disease without COVID-19 (Me age-32 years) with different disease activity according to the Meio activity index (group 1) and 23 UC patients with COVID-19 in the acute period (group 2) were examined. -a1-antitrypsin (AAT) (a1-Antitrypsin Clearance ELISA Immundiagnostik, Germany) was determined in blood serum and in feces. Statistical processing was carried out using the Statistica 6.0 program. Results: In the blood serum of UC patients with exacerbation of the disease without COVID-19, AAT fluctuations from 355 to 3056 mcg/l (norm 900-1800 mcg/l) were detected, the average value was 847.9616.5 mcg/l. In UC patients with exacerbation of the disease and the presence of COVID-19 in the acute period, AAT fluctuations from 645 to 4896 mcg/l (norm 900-1800 mcg/l), the average value is 2286.3 6181.4 mcg/l (P 5 0.000000). The range of AAT concentration in feces ranged from 55 to 625 ml/g of feces (norm 25-35 mg/g of feces), on average 364.9 6 11.4 ml/g of feces. The range of AAT concentration in feces ranged from 112 to 898 ml/g feces) (norm 25-35 mg/g feces), on average 539.6618.4 ml/g feces (P 5 0.000000). Conclusion(s):The increase in AAT in the blood serum and in the feces of patients with exacerbation of UC with the presence of COVID-19 infection in the acute period is significantly higher in patients with UC without COVID-19, which indicates a higher permeability of the intestinal wall.