S19.1. Effect of shear stress on IL-1β induced upregulation of icam-1 on huvec

Sampath, Rangarajan; Smith, C. Wayne; Eskin, S. G.; McIntire, L.V.

doi:10.1016/0006-355x(95)92052-c

Cited by 3 publications

(6 citation statements)

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“…This has been described in pincer impingement, whereby persistent anterosuperior abutment of the femur against the acetabular rim causes chronic levering of the femur and microinstability posteroinferiorly. 54,55 In this scenario, greater shear stresses, which are known to be particularly unfavorable to cartilage metabolism, 56 are experienced posteroinferiorly. Consistent with our findings, a cadaveric biomechanical study suggested that a tendency to femoral retroversion distributes higher hip contact pressures posteroinferiorly, possibly due to abutment anterosuperiorly producing the described mild levering effect and "contrecoup" damage posteroinferiorly.…”

Section: Resultsmentioning

confidence: 99%

Combined femoral and acetabular version and synovitis are associated with dGEMRIC scores in people with femoroacetabular impingement (FAI) syndrome

Murphy

Eyles

Spiers

et al. 2023

Journal Orthopaedic Research

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This study sought to explore, in people with symptoms, signs and imaging findings of femoroacetabular impingement (FAI syndrome): (1) whether more severe labral damage, synovitis, bone marrow lesions, or subchondral cysts assessed on magnetic resonance imaging (MRI) were associated with poorer cartilage health, and (2) whether abnormal femoral, acetabular, and/or combined femoral and acetabular versions were associated with poorer cartilage health. This cross‐sectional study used baseline data from the 50 participants with FAI syndrome in the Australian FASHIoN trial (ACTRN12615001177549) with available dGEMRIC scans. Cartilage health was measured using delayed gadolinium‐enhanced MRI of cartilage (dGEMRIC) score sampled at the chondrolabral junction on three midsagittal slices, at one acetabular and one femoral head region of interest on each slice, and MRI features were assessed using the Hip Osteoarthritis MRI Score. Analyses were adjusted for alpha angle and body mass index, which are known to affect dGEMRIC score. Linear regression assessed the relationship with the dGEMRIC score of (i) selected MRI features, and (ii) femoral, acetabular, and combined femoral and acetabular versions. Hips with more severe synovitis had worse dGEMRIC scores (partial η2 = 0.167, p = 0.020), whereas other MRI features were not associated. A lower combined femoral and acetabular version was associated with a better dGEMRIC score (partial η2 = 0.164, p = 0.021), whereas isolated measures of femoral and acetabular version were not associated. In conclusion, worse synovitis was associated with poorer cartilage health, suggesting synovium and cartilage may be linked to the pathogenesis of FAI syndrome. A lower combined femoral and acetabular version appears to be protective of cartilage health at the chondrolabral junction.

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Section: Resultsmentioning

confidence: 99%

Combined femoral and acetabular version and synovitis are associated with dGEMRIC scores in people with femoroacetabular impingement (FAI) syndrome

Murphy

Eyles

Spiers

et al. 2023

Journal Orthopaedic Research

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“…Consequently, the application of mechanical loading including shear, intermittent compression, 2–4 hydrostatic pressure, 5,6 and cyclic tensile strain 7–9 as a tool for enhancing ECM deposition in vitro has occupied significant interest in the scientific community. During body movement, the compressive load on AC leads to the displacement of 80% of the water contained within the ECM, which flows back into the ECM when the joint is unloaded by the action of osmotic pressure and the attraction of negatively charged glycosaminoglycan chains (GAGs), which make up nearly 30% of AC ECM dry weight 10–12 . The flow of water within the ECM during loading and unloading applies an interstitial shear stress on AChs affecting their homeostasis as evident by an ECM composition change upon exposure to interstitial shear in the work by Chen et al 13 Therefore, studying the positive effects of interstitial flow on ACh viability, expression of genes encoding for production of chondrogenic ECM components, and others encoding for the inflammatory responses in AC will enhance the understanding of potential mechanisms for mediating AC diseases, like osteoarthritis (OA) and rheumatoid arthritis (RA), and in exploring important regulator factors within the field of AC tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Impact of interstitial flow on cartilage matrix synthesis and NF‐kB transcription factor mRNA expression in a novel perfusion bioreactor

Abusharkh,

Robertson,

Mendenhall

et al. 2023

Biotechnology Progress

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This work is focused on designing an easy‐to‐use novel perfusion system for articular cartilage (AC) tissue engineering and using it to elucidate the mechanism by which interstitial shear upregulates matrix synthesis by articular chondrocytes (AChs). Porous chitosan‐agarose (CHAG) scaffolds were synthesized and compared to bulk agarose (AG) scaffolds. Both scaffolds were seeded with osteoarthritic human AChs and cultured in a novel perfusion system with a medium flow velocity of 0.33 mm/s corresponding to 0.4 mPa surfice shear and 40 mPa CHAG interstitial shear. While there were no statistical differences in cell viability for perfusion versus static cultures for either scaffold type, CHAG scaffolds exhibited a 3.3‐fold higher (p < 0.005) cell viability compared to AG scaffold cultures. Effects of combined superficial and interstitial perfusion for CHAG showed 150‐ and 45‐fold (p < 0.0001) increases in total collagen (COL) and 13‐ and 2.2‐fold (p < 0.001) increases in glycosaminoglycans (GAGs) over AG non‐perfusion and perfusion cultures, respectively, and a 1.5‐fold and 3.6‐fold (p < 0.005) increase over non‐perfusion CHAG cultures. Contrasting CHAG perfusion and static cultures, chondrogenic gene comparisons showed a 3.5‐fold increase in collagen type II/type I (COL2A1/COL1A1) mRNA ratio (p < 0.05), and a 1.3‐fold increase in aggrecan mRNA. Observed effects are linked to NF‐κB signal transduction pathway inhibition as confirmed by a 3.2‐fold (p < 0.05) reduction of NF‐κB mRNA expression upon exposure to perfusion. Our results demonstrate that pores play a critical role in improving cell viability and that interstitial flow caused by medium perfusion through the porous scaffolds enhances the expression of chondrogenic genes and extracellular matrix through downregulating NF‐κB1.

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“…Chondrocytes are known to respond to mechanical stimuli, particularly in articular cartilage, and these stimuli may be converted into a mechanotransduction signal to increase chondrogenesis and/or proliferation which can be exploited in vitro (Lane Smith et al, 2000;Millward-Sadler and Salter, 2004;Shahin and Doran, 2015). Mechanical stresses play a role at all stages of cartilage development:…”

Section: Mechanical Forces and Bioreactorsmentioning

confidence: 99%

“…Whilst most research has focussed on articular cartilage, there are a limited number of studies that indicate facial cartilages also have the capacity to respond favourably to hydrostatic pressure and compressive loading, but that these responses may differ between different facial cartilages such as temporomandibular and nasoseptal cartilage (Takano-Yamamoto et al, 1991;Correro-Shahgaldian et al, 2016). Shear stress in contrast, has the potential to evoke apoptotic and osteogenic changes in cartilage at levels in excess of 1 Pa, and is believed to contribute to osteoarthritic changes in vivo, making this an important force to modulate during tissue engineering and bioprinting processes (Lane Smith et al, 2000;Smith, Carter and Schurman, 2004;Sharifi and Gharravi, 2019). The ability to control the environment in regard to mechanical forces has as such promoted the development of dynamic culture conditions in the form of bioreactor systems that attempt to maximise extracellular matrix production in tissue engineered cartilage (K. Sharifi and Gharravi, 2019;Fu et al, 2021).…”

Section: Mechanical Forces and Bioreactorsmentioning

confidence: 99%

“…In articular cartilage, high shear stress is believed to underpin osteoarthritic changes, whereas low levels of shear stress are believed to contribute to normal homeostatic mechanisms (Lane Smith et al, 2000). This shear stress response is mediated through the pericellular matrix, primary cilium and stretch activated calcium channels on the surface membrane, with TGFβ1 implicated in degenerative changes (Zhao et al, 2020).…”

Section: )mentioning

confidence: 99%

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Development of a Novel Composite Bioink for Cartilage Tissue Engineering

Jovic¹

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Summary Introduction and aims: Nanocellulose bioinks have promising biological and mechanical properties for 3D bioprinting. The aim of this thesis was to develop a novel natural composite biomaterial derived of nanocellulose to bioprint with cartilage derived cells for ear reconstruction. Methods: Cartilage derived cells were extracted from nasoseptal cartilage and chondroprogenitor cells were isolated using fibronectin adhesion assay. Different combinations of progenitor cells and chondrocytes were created to determine the most chondrogenic combination for 3D bioprinting cartilage. Nanocellulose blend, crystal and fibrils were blended with alginate and compared for printability and chondrogenicity profiles. The most chondrogenic and printable formulation was then mixed with varying proportions of hyaluronic acid to produce composite bioinks. Human nasoseptal chondrocytes from at least 3 separate patients were cultured in the composite biomaterial, and crosslinking with low dose hydrogen peroxide was optimised. Chondrogenicity was determined with PCR, quantitative protein assays and histology. Printability was assessed using rheology and printing assays. Mechanical properties were examined using atomic force microscopy and compression testing. Biocompatibility was demonstrated with Live-Dead, lactate dehydrogenase and alamarBlue assays. Conclusions: There were no benefits to combining cells isolated with the fibronectin assay compared to using native cartilage cell populations, indicating an inadequacy with the validity of this assay in nasoseptal cartilage. All nanocellulose materials demonstrated superior printability and bicompatibilty to alginate, with crystals and blend varieties offering superior biological properties. Instant crosslinking of the nanocellulose-hyaluronic acid bioinks were achievable with no detriment to cell survival. Nanocellulosehyaluronic acid bioink was more chondrogenic than nanocellulose-alginate and hyaluronic acid alone. Cell viability and proliferation was sustained over 21 days. Nanocellulose-hyaluronic acid bioinks demonstrate superior chondrogenicity, favourable mechanical properties and excellent biocompatibility for bioprinting cartilage for reconstructive surgery. These inks hold promise for in vivo testing and eventually clinical translation.

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S19.1. Effect of shear stress on IL-1β induced upregulation of icam-1 on huvec

Cited by 3 publications

References 0 publications

Combined femoral and acetabular version and synovitis are associated with dGEMRIC scores in people with femoroacetabular impingement (FAI) syndrome

Combined femoral and acetabular version and synovitis are associated with dGEMRIC scores in people with femoroacetabular impingement (FAI) syndrome

Impact of interstitial flow on cartilage matrix synthesis and NF‐kB transcription factor mRNA expression in a novel perfusion bioreactor

Development of a Novel Composite Bioink for Cartilage Tissue Engineering

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