S100B protein stimulates calcineurin activity

Leal, Rodrigo Bainy; Frizzo, Juliana Karl; Tramontina, Francine; Fieuw-Makaroff, Sabine; Bobrovskaya, Larisa; Dunkley, Peter R.; Gonçalves, Carlos-Alberto

doi:10.1097/00001756-200402090-00021

Cited by 15 publications

(5 citation statements)

References 20 publications

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“…This is in agreement with studies where animals subjected to chronic social stress, with impairment in long-term potentiation in the hippocampus, show higher basal levels of calcineurin [32]. Furthermore, calcineurin is also increased by S-100B in a Ca 2+ -dependent manner [33]. Calcineurin (protein phosphatase 2B), is however also involved in neuronal plasticity [34] and so it was anticipated that changes in the expression of proteins participating in processes important for neural plasticity, would be present in rats subjected to trauma.…”

Section: Discussionsupporting

confidence: 92%

Large Scale Hippocampal Cellular Distress may Explain the Behavioral Consequences of Repetitive Traumatic Experiences-A Proteomic Approach

et al. 2008

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Early life traumatic experiences are associated with psychopathology in adulthood. This may be due in part to the effects of trauma on hippocampal development and protein expression. The purpose of the study was to investigate the effects of early life trauma and adult re-stress on ventral hippocampal protein expression. Adolescent rats (n = 19) were subjected to a triple stressor on post-natal day 28 followed 7 days later by the first re-stress session and 25 days later (post-natal day 60 = adulthood) by the second re-stress session. Ventral hippocampi were collected on post-natal day 68 for protein expression determinations using protein arrays and 2D-gel electrophoresis with liquid chromatography tandem mass spectrometry. Compared to controls, traumatized animals showed an increase in Ca(2+) homeostatic proteins, dysregulated signaling pathways and energy metabolism enzymes, cytoskeletal protein changes, a decrease in neuroplasticity regulators, energy metabolism enzymes and an increase in apoptotic initiator proteins. These results indicate the extensive impact of trauma on adult brain development and behavior.

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Section: Discussionsupporting

confidence: 92%

Large Scale Hippocampal Cellular Distress may Explain the Behavioral Consequences of Repetitive Traumatic Experiences-A Proteomic Approach

et al. 2008

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“…ERK, Extracellular-Regulated Kinase; IQGAP1, IQ motif-containing GTPase-Activating Protein type 1; MAP, Microtubule Associated Protein; MARCKS, Myristoylated Alanine-Rich C Kinase Substrate; NDR kinase, Nuclear Dbf2 Related Kinase; NF-κB, Nuclear Factor kappa B; iNOS, inducible Nitric Oxide Synthase; PKC, Protein Kinase C; LPA, LysoPhosphatidic Acid; RAGE, Receptor for Advanced Glycation End products; STZ, Streptozotocin. Letters indicate supporting references: a [3]; b [4]; c [5]; d [6]; e [7]; f [8]; g [9]; h [10]; i [11]; j [12]; k [13]; l [14]; m [15]; n [16]; o [17]; p [18]; q [19]; r [20]; s [21]; t [22]; u [23]; v [24]; x [25]; and z [26]. in gray matter, whilst oligodendrocytes are the predominant S100B-positive cells in white matter [47].…”

Section: Introductionmentioning

confidence: 99%

Biological and methodological features of the measurement of S100B, a putative marker of brain injury

Gonçalves

Leite

Nardin

2008

Clinical Biochemistry

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228

169

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“…In multiple cell lines and primary cultures, CN activity is robustly recruited by several cytokine factors and endogenous inflammatory mediators [8, 16, 28, 32, 33]. Additionally, serum, like cytokines, triggers the upregulation of well-characterized inflammatory biomarkers including COX-2 and prostaglandins [24].…”

Section: Discussionmentioning

confidence: 99%

Disparate effects of serum on basal and evoked NFAT activity in primary astrocyte cultures

Furman

Artiushin

Norris

2010

Neuroscience Letters

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In astrocytes, the Ca2+-dependent protein phosphatase calcineurin (CN) strongly regulates neuro-immune/inflammatory cascades through activation of the transcription factor, nuclear factor of activated T cells (NFAT). While primary cell cultures provide a useful model system for investigating astrocytic CN/NFAT signaling, variable results may arise both within and across labs because of differences in culture conditions. Here, we determined the extent to which serum and cell confluency affect basal and evoked astrocytic NFAT activity in primary cortical astrocyte cultures. Cells were grown to either ~50% or >90% confluency, pre-loaded with an NFAT-luciferase reporter construct, and maintained for 16 h in medium with or without 10% fetal bovine serum (FBS). NFAT-dependent luciferase expression was then measured 5 h after treatment with vehicle alone to assess basal NFAT activity, or with Ca2+ mobilizers and IL-1β to assess evoked activity. The results revealed significantly higher levels of basal NFAT activity in FBS-containing medium, regardless of cell confluency. Conversely, evoked NFAT activation was significantly lower in serum-containing medium, with an even greater inhibition observed in confluent cultures. Application of 10% FBS to serum-free astrocyte cultures quickly evoked a roughly seven-fold increase in NFAT activity that was significantly reduced by co-delivery of neutralizing agents for IL-1β, TNFα, and/or IFNγ, suggesting that serum occludes evoked NFAT activation through a cytokine-based mechanism. Together, the results demonstrate that the presence of serum and cell confluency have a major impact on CN/NFAT signaling in primary astrocyte cultures and therefore must be taken into consideration when using this model system.

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S100B protein stimulates calcineurin activity

Cited by 15 publications

References 20 publications

Large Scale Hippocampal Cellular Distress may Explain the Behavioral Consequences of Repetitive Traumatic Experiences-A Proteomic Approach

Large Scale Hippocampal Cellular Distress may Explain the Behavioral Consequences of Repetitive Traumatic Experiences-A Proteomic Approach

Biological and methodological features of the measurement of S100B, a putative marker of brain injury

Disparate effects of serum on basal and evoked NFAT activity in primary astrocyte cultures

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