S100A6 expression in keratinocytes and its impact on epidermal differentiation

Graczyk, Agnieszka; Leśniak, Wiesława

doi:10.1016/j.biocel.2014.10.007

Cited by 22 publications

(21 citation statements)

References 36 publications

(41 reference statements)

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“…It is widely considered that serial subculture of NHEK resulted in replicative senescence and terminal differentiation[13, 14]. Another cellular process may predict the relationship between K24 and differentiation is calcium-mediated signal transduction, a potent inducer of keratinocyte differentiation[15]. Here we showed that K24 was up-regulated in senescent keratinocytes upon subculture and CaCl 2 -induced differentiation.…”

Section: Discussionmentioning

confidence: 48%

Role of keratin 24 in human epidermal keratinocytes

et al. 2017

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Keratin 24 (K24) is a new kind of keratin genes, which encodes a novel keratin protein, K24 that bears high similarity to the type I keratins and displays a unique expression profile. However, the role of K24 is incompletely understood. In our study, we investigated the localization of K24 within the epidermis and possible functions. Keratin 24 was found to be modestly overexpressed in senescent keratinocytes and was mainly restricted to the upper stratum spinosum of epidermis. The protein was required for terminal differentiation upon CaCl2-induced differentiation. In vitro results showed that increased K24 in keratinocytes dramatically changed the differentiation of primary keratinocytes. It also inhibited cell survival by G1/S phase cell cycle arrest and induced senescence, autophagy and apoptosis of keratinocytes. In addition, K24 activated PKCδ signal pathway involving in cellular survival. In summary, K24 may be suggested as a potential differentiation marker and anti-proliferative factor in the epidermis.

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Section: Discussionmentioning

confidence: 48%

Role of keratin 24 in human epidermal keratinocytes

et al. 2017

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“…This result revealed that keratinocytes deficient for HSPA2 expression started to form granular layer faster than the control cells. Since LOR is expressed at later stages of HaCaT cell differentiation in RHE model (Graczyk & Leśniak, ; Schoop, Mirancea, & Fusenig, ), we did not detect this antigen neither in control nor in HSPA2‐deficient RHE (not shown). Thus, using RHE models we were able to show that HSPA2 is involved in the spatiotemporal control of keratinocyte differentiation during the organization of multilayered epidermis in vitro.…”

Section: Resultsmentioning

confidence: 78%

Novel role for the testis‐enriched HSPA2 protein in regulating epidermal keratinocyte differentiation

Gogler-Pigłowska

Klarzyńska

Sojka

et al. 2017

Journal Cellular Physiology

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HSPA2, a poorly characterized member of the HSPA (HSP70) chaperone family, is a testis-enriched protein involved in male germ cell differentiation. Previously, we revealed that HSPA2 is present in human stratified epithelia, including epidermis, however the contribution of this protein to epithelial biology remained unknown. Here, we show for the first time that HSPA2 is expressed in basal epidermal keratinocytes, albeit not in keratinocytes exhibiting features attributed to primitive undifferentiated progenitors, and participates in the keratinocyte differentiation process. We found that HSPA2 is dispensable for protection of HaCaT keratinocytes against heat shock-induced cytotoxicity. We also shown that lentiviral-mediated shRNA silencing of HSPA2 expression in HaCaT cells caused a set of phenotypic changes characteristic for keratinocytes committed to terminal differentiation such as reduced clonogenic potential, impaired adhesiveness and increased basal and confluency-induced expression of differentiation markers. Moreover, the fraction of undifferentiated cells that rapidly adhered to collagen IV was less numerous in HSPA2-deficient cells than in the control. In a 3D reconstructed human epidermis model, HSPA2 deficiency resulted in accelerated development of a filaggrin-positive layer. Collectively, our results clearly show a link between HSPA2 expression and maintenance of keratinocytes in an undifferentiated state in the basal layer of the epidermis. It seems that HSPA2 could retain keratinocytes from premature entry into the terminal differentiation process. Overall, HSPA2 appears to be necessary for controlling development of properly stratified epidermis and thus for maintenance of skin homeostasis.

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“…The cornified envelope formation of the skin also requires the differentiation of keratinocytes (61,68). This differentiation is regulated by the epidermal differentiation complex (EDC).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Atopic Dermatitis and Diet on the Skin Transcriptome in Staffordshire Bull Terriers

et al. 2020

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Canine atopic dermatitis (CAD) has a hereditary basis that is modified by interactions with the environment, including diet. Differentially expressed genes in non-lesional skin, determined by RNA sequencing before and after a dietary intervention, were compared between dogs with naturally occurring CAD (n = 4) and healthy dogs (n = 4). The dogs were fed either a common commercial heat-processed high carbohydrate food (kibble diet) (n = 4), or a non-processed high fat food (raw meat-based diet) (n = 4). At the end of the diet intervention, 149 differentially expressed transcripts were found between the atopic and healthy dogs. The main canonical pathways altered by the dysregulation of these genes were angiopoietin signaling, epidermal growth factor signaling, activation of angiogenesis, and alterations in keratinocyte proliferation and lipid metabolism. On the other hand, 33 differently expressed transcripts were found between the two diet groups, of which 8 encode genes that are annotated in the current version of the dog genome: immunoglobulin heavy constant mu (IGHM), immunoglobulin lambda-like polypeptide 5 (IGLL5), B-cell antigen receptor complex-associated protein beta chain (CD79B), polymeric immunoglobulin receptor (PIGR), cystathionine β-synthase (CBS), argininosuccinate synthase 1 (ASS1), secretory leukocyte peptidase inhibitor (SLPI), and mitochondrial ribosome recycling factor (MRRF). All genes were upregulated in the raw diet group. In conclusion the findings of this study suggest alterations in lipid and keratinocyte metabolism as well as angiogenesis in the skin of atopic dogs. Additionally, a possible enhancement of innate immunity and decrease in oxidative stress was seen in raw food fed dogs, which could have an important role in preventing hypersensitivities and disturbed immunity at young age.

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S100A6 expression in keratinocytes and its impact on epidermal differentiation

Cited by 22 publications

References 36 publications

Role of keratin 24 in human epidermal keratinocytes

Role of keratin 24 in human epidermal keratinocytes

Novel role for the testis‐enriched HSPA2 protein in regulating epidermal keratinocyte differentiation

The Effect of Atopic Dermatitis and Diet on the Skin Transcriptome in Staffordshire Bull Terriers

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