S. Typhimurium virulence changes caused by exposure to different non-thermal preservation treatments using C. elegans

Sanz-Puig, Maria; Lázaro, Elena; Pardo, Leandro; Álvares, Danilo; Martínez, Antonio; Rodrigo, Dolores

doi:10.1016/j.ijfoodmicro.2017.09.006

Cited by 9 publications

(7 citation statements)

References 29 publications

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“…All inferential processes agree with the conclusions in Sanz‐Puig et al. (2017) that the cauliflower by‐product infusion can be an alternative preservation treatment. This fact evidences the robustness (regardless of the

h_{0} false(t false)

specification) of the Cox model in estimating covariate effects.…”

Section: Discussionsupporting

confidence: 87%

“…ST non‐treated ( ST0 ), ST treated once ( ST1 ), and ST treated three times ( ST3 ) was the source of nutrition of 250 synchronized young adult nematodes kept in identical environmental conditions throughout their lifespan (approximately three weeks at the most). Virulence for each worm was defined in terms of their survival time (see Sanz‐Puig et al., 2017, for more details about the validation and special conditions of the study). Most of the data were fully observed.…”

Section: An Experiments On Microbial Virulencementioning

confidence: 99%

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Bayesian regularization for flexible baseline hazard functions in Cox survival models

2020

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Fully Bayesian methods for Cox models specify a model for the baseline hazard function. Parametric approaches generally provide monotone estimations. Semi‐parametric choices allow for more flexible patterns but they can suffer from overfitting and instability. Regularization methods through prior distributions with correlated structures usually give reasonable answers to these types of situations. We discuss Bayesian regularization for Cox survival models defined via flexible baseline hazards specified by a mixture of piecewise constant functions and by a cubic B‐spline function. For those “semi‐parametric” proposals, different prior scenarios ranging from prior independence to particular correlated structures are discussed in a real study with microvirulence data and in an extensive simulation scenario that includes different data sample and time axis partition sizes in order to capture risk variations. The posterior distribution of the parameters was approximated using Markov chain Monte Carlo methods. Model selection was performed in accordance with the deviance information criteria and the log pseudo‐marginal likelihood. The results obtained reveal that, in general, Cox models present great robustness in covariate effects and survival estimates independent of the baseline hazard specification. In relation to the “semi‐parametric” baseline hazard specification, the B‐splines hazard function is less dependent on the regularization process than the piecewise specification because it demands a smaller time axis partition to estimate a similar behavior of the risk.

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h_{0} false(t false)

specification) of the Cox model in estimating covariate effects.…”

Section: Discussionsupporting

confidence: 87%

Section: An Experiments On Microbial Virulencementioning

confidence: 99%

Bayesian regularization for flexible baseline hazard functions in Cox survival models

2020

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“…It has also been found that many vegetables in the Cruciferae family have antimicrobial properties against several microorganisms of clinical importance. The in vitro antibacterial effect of Brassica oleracea products on several foodborne pathogens was evidenced by Brandi et al (2006) and Sanz-Puig et al (2017), between others. Some of the antimicrobial properties of these plant extracts are associated with antioxidant compounds such as polyphenols (Sanz-Puig et al, 2015b;Marchese et al, 2016;Bakari et al, 2018).…”

Section: Introductionmentioning

confidence: 88%

In vivo Antimicrobial Activity Assessment of a Cauliflower By-Product Extract Against Salmonella Typhimurium

Ibañez-Peinado

Pina-Pérez

García-Carrión

et al. 2020

Front. Sustain. Food Syst.

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The main objective of this work was to study the antimicrobial effect of a cauliflower by-product infusion into an affordable in vivo model (Caenorhabditis elegans). The infusion demonstrated some protective effect on non-infected and infected worms with Salmonella Typhimurium as indicated by higher survival percentile values (75, 50, 25, and 5% percentiles) as compared with those from worms unexposed to the infusion. The antimicrobial effect of the infusion was evaluated on Salmonella intestinal colonization of infected worms (24, 48, and 96 h post-infection). At 96 h post-infection, the concentration of Salmonella was reduced around 2 log cycles in infected cauliflower treated group (p < 0.05) as compared with infected non-cauliflower group. Here we show that cauliflower by-products extend survival and have an antimicrobial effect in an in vivo nematode model, C. elegans, as a previous validation step to longer and costlier farm animal studies.

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“…Caenorhabditis elegans has been used very frequently as a model of pathogenesis, and in particular for infection by pathogenic microorganisms ( Balla and Troemel, 2013 ; Curt et al, 2014 ). In this sense, there are published studies in which the antimicrobial activity of a compound has been determined based on a higher survival of infected C. elegans when exposed to the antimicrobial compounds when compared with infected control nematodes (non-exposed to the antimicrobial compound) ( Sanz-Puig et al, 2017 ; Ibáñez-Peinado et al, 2020 ; Palacios-Gorba et al, 2020 ). In the present study, an independent set of experiments was specifically designed to study the Spirulina (treated and untreated by CP) antimicrobial activity against Salmonella enterica serovar Typhimurium when C. elegans worms were infected.…”

Section: Resultsmentioning

confidence: 99%

In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina

et al. 2022

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The present study challenges the in vivo assessment of cold atmospheric pressure plasma (CAPP) technology on the bioactive activity (antioxidant/antiaging and antimicrobial potential) of Spirulina powder, using Caenorhabditis elegans as an animal model. Surface microdischarge cold atmospheric pressure plasma (SMD-CAPP) treatment was 3.3 W discharge power for 7 min. C. elegans lifespan and egg laying were used as indicators of antioxidant/antiaging potential of Spirulina (1 mg/mL), when grown with Spirulina CP-treated [E_SCP] and untreated [E_S], compared with a control [E_0] (non-supplemented with Spirulina). According to our results, under both Spirulina supplemented media [E_SCP and E_S] and for the first 17 days, nematodes experienced an increase in lifespan but without significant differences (p > 0.05) between control and Spirulina CP-treated. Regarding the in vivo assay of the antimicrobial potential of Spirulina against Salmonella enterica serovar Typhimurium (infected worms), no significant differences (p > 0.05) were found between the three exposure scenarios (control [S_0]; Spirulina supplemented media [S_S]; CP-treated Spirulina supplemented media [S_SCP]). According to present results, CAPP-treatment do not influence negatively the lifespan of C. elegans but a reduction in the Spirulina antiaging potential was found. No in vivo modifications in antimicrobial activity seem to be linked to CAPP-processed Spirulina.

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S. Typhimurium virulence changes caused by exposure to different non-thermal preservation treatments using C. elegans

Cited by 9 publications

References 29 publications

Bayesian regularization for flexible baseline hazard functions in Cox survival models

Bayesian regularization for flexible baseline hazard functions in Cox survival models

In vivo Antimicrobial Activity Assessment of a Cauliflower By-Product Extract Against Salmonella Typhimurium

In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina

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