S. S. D. Jones

Stringer, Freddy

doi:10.1017/s0373463300014107

Cited by 11 publications

(23 citation statements)

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“…Srpt5 was present in all four spacers and other data (Stringer et al 1991; Ambrose et al 2004). of subtelomere motifs between genes suggests that All of the repeated genes in the cloned arrays were genes might have been inserted into one or more subtelpointed in the same direction, toward the telomere.…”

Section: General Structure Of Sequenced Telomeric Gene Arrayssupporting

confidence: 73%

Gene Arrays atPneumocystis cariniiTelomeres

et al. 2005

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In the fungus Pneumocystis carinii, at least three gene families (PRT1, MSR, and MSG) have the potential to generate high-frequency antigenic variation, which is likely to be a strategy by which this parasitic fungus is able to prolong its survival in the rat lung. Members of these gene families are clustered at chromosome termini, a location that fosters recombination, which has been implicated in selective expression of MSG genes. To gain insight into the architecture, evolution, and regulation of these gene clusters, six telomeric segments of the genome were sequenced. Each of the segments began with one or more unique genes, after which were members of different gene families, arranged in a head-to-tail array. The three-gene repeat PRT1-MSR-MSG was common, suggesting that duplications of these repeats have contributed to expansion of all three families. However, members of a gene family in an array were no more similar to one another than to members in other arrays, indicating rapid divergence after duplication. The intergenic spacers were more conserved than the genes and contained sequence motifs also present in subtelomeres, which in other species have been implicated in gene expression and recombination. Long mononucleotide tracts were present in some MSR genes. These unstable sequences can be expected to suffer frequent frameshift mutations, providing P. carinii with another mechanism to generate antigen variation.

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Section: General Structure Of Sequenced Telomeric Gene Arrayssupporting

confidence: 73%

Gene Arrays atPneumocystis cariniiTelomeres

et al. 2005

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“…Clustering of MSG genes in rat-derived P. carinii has been inferred by application of the polymerase chain reaction [37]. To better understand the structure and organization of MSG genes in rat-derived P. carinii, we used DNA hybridization and sequence determination to characterize two MSG genes that are organized as a tandem repeat in a lambda phage clone (Rp3-I); which we had previously characterized as containing sequences that were repeated in the genome of rat-derived P. carinii [33]. Using the sequences of the two MSG genes, PCR primers were designed in order to amplify intergenic regions between tandem MSG gene pairs.…”

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confidence: 99%

“…Proteinase K (Sigma) was added to 25 mum], followed by incubation at 55" C for 18 h. DNA was extracted by phenolchloroform, then precipitated at -20" C for 18 h by the addition of 2.5 volumes of absolute ethanol and 1/10 volume of 3 M sodium acetate (pH 6.5) [26]. DNA was collected by centrifugation at 12,000 g for 15 min and the pelleted DNA was resuspended in 20 pi of TE (0.01 M Tris-HC1 (pH 8.3), 0.001 M EDTA) (261.X phage Rp3-1 had been isolated from a rat-derived P. carinii genomic library as previously described [33]. SacI F, E,, and E2 and EcoRI C are subclones of X Rp3-1 [33].…”

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confidence: 99%

“…DNA was collected by centrifugation at 12,000 g for 15 min and the pelleted DNA was resuspended in 20 pi of TE (0.01 M Tris-HC1 (pH 8.3), 0.001 M EDTA) (261.X phage Rp3-1 had been isolated from a rat-derived P. carinii genomic library as previously described [33]. SacI F, E,, and E2 and EcoRI C are subclones of X Rp3-1 [33]. SacI G was subcloned into Bluescript KS+ (Strategene Cloning Systems, LaJolla, CA) as previously described [33].…”

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confidence: 99%

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A Tandem Repeat of Rat‐derived Pneumocystis carinii Genes Encoding the Major Surface Glycoprotein

Sunkin

Stringer

1994

J Eukaryotic Microbiology

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A fragment from the genome of rat-derived Pneumocystis carinii was found to contain two MSG genes arranged as a direct repeat. The sequences from one gene (MSG B), the region between the two genes, and part of the second gene (MSG A) were determined. The two MSG genes were not identical in sequence. The open reading frames of MSG A and MSG B encode non-identical proteins, both of which are similar to that encoded by a previously published cDNA. The MSG B gene sequence showed no evidence of introns. The 5' and 3' untranslated regions of the MSG gene pair were highly conserved, but the regions immediately upstream of the open reading frames of MSG A and B were different from the region upstream of a previously characterized MSG cDNA. Primers designed to extend upstream of the 5' end of MSG and downstream of the 3' end of MSG were used in a polymerase chain reaction with total genomic P. carinii DNA as template. Presumptive intergenic amplification products from this reaction were cloned and sequenced. The sequences of these regions were similar but distinct, indicating that tandem arrangement of MSG genes is a common organizational motif.

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“…carinii organisms express a surface antigen known as the major surface glycoprotein (MSG) (Walzer & Linke, 1987;Kovacs et al, 1988;Tanabe et al, 1989;Nakamura, 1998;. Multiple forms of MSG are encoded by a family of approximately 100 genes, members of which are located at 34 telomeric sites (Stringer et al, 1991;Kovacs et al, 1993;Sunkin et al, 1994;Sunkin & Stringer, 1996, 1997Cornillot et al, 2002;Keely et al, 2003). Other species of Pneumocystis, including the species that infects humans, also have families of MSG genes (Haidaris et al, 1991;Stringer et al, 1993;Garbe & Stringer, 1994;Wright et al, 1995;Mei et al, 1998;Haidaris et al, 1998;Schaffzin et al, 1999;Kutty et al, 2001).…”

Section: Introductionmentioning

confidence: 99%