S‐nitrosylation of cytoskeletal proteins

Horenberg, Allison L; Houghton, Alisa M; Pandey, S.; Seshadri, Vikram; Guilford, William H.

doi:10.1002/cm.21520

Cited by 20 publications

(23 citation statements)

References 112 publications

(158 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The accumulating data show that the SNO of cytoskeletal proteins selectively alters their function. It complements the effects of cGMP on cytoskeletal proteins in the cells, modulating cell migration and contractility [11]. Accordingly, it has been shown that the proteostasis of actin and tubulin proteins is precisely controlled during neuronal maturation in the brain, and NO signaling appears to be a key regulator of these processes [63].…”

Section: Discussionmentioning

confidence: 99%

“…At some cysteines, it can be driven by a putative "nitrosylation motif" in the primary structure of Biomedicines 2020, 8, 124 2 of 20 nitrosoproteins [8,10]. Such motifs could be incorporated into the three-dimensional structure of cysteines rather than in the primary structure [11]. SNO plays a signaling role in regulation of protein localization, axonal transport, synaptic plasticity and various neuronal pathways [12,13].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sex Differences in Biological Processes and Nitrergic Signaling in Mouse Brain

2020

View full text Add to dashboard Cite

Nitric oxide (NO) represents an important signaling molecule which modulates the functions of different organs, including the brain. S-nitrosylation (SNO), a post-translational modification that involves the binding of the NO group to a cysteine residue, is a key mechanism of nitrergic signaling. Most of the experimental studies are carried out on male animals. However, significant differences exist between males and females in the signaling mechanisms. To investigate the sex differences in the SNO-based regulation of biological functions and signaling pathways in the cortices of 6–8-weeks-old mice, we used the mass spectrometry technique, to identify S-nitrosylated proteins, followed by large-scale computational biology. This work revealed significant sex differences in the NO and SNO-related biological functions in the cortices of mice for the first-time. The study showed significant SNO-induced enrichment of the synaptic processes in female mice, but enhanced SNO-related cytoskeletal processes in the male mice. Proteins, which were S-nitrosylated in the cortices of mice of both groups, were more abundant in the female brain. Finally, we investigated the shared molecular processes that were found in both sexes. This study presents a mechanistic insight into the role of S-nitrosylation in both sexes and provides strong evidence of sex difference in many biological processes and signalling pathways, which will open future research directions on sex differences in neurological disorders.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sex Differences in Biological Processes and Nitrergic Signaling in Mouse Brain

2020

View full text Add to dashboard Cite

show abstract

“…NO bioactivity has also been associated with sGC‐independent effects, via covalent post‐translational modification of target proteins including S‐nitrosylation (ie binding of a NO group to a transition metal or cysteine residues and forming an nitrosothiol moiety), as well as tyrosine nitration (ie binding of a NO 2 group to a tyrosine or tryptophan residue) of proteins as well as unsaturated fatty acids . Although some physiological roles have been reported for S‐nitrosylation, sGC‐independent effects mainly occur at high concentrations (ie µmol L −1 ‐mmol L −1 range) of NO and mostly mediate its detrimental effects . This issue is unlike sGC‐dependent physiological effects of NO which occur in the presence of low nanomolar concentrations of NO …”

Section: No Receptormentioning

confidence: 99%

Nitric oxide: To be or not to be an endocrine hormone?

et al. 2020

View full text Add to dashboard Cite

Nitric oxide (NO), a highly reactive gasotransmitter, is critical for a number of cellular processes and has multiple biological functions. Due to its limited lifetime and diffusion distance, NO has been mainly believed to act in autocrine/paracrine fashion. The increasingly recognized effects of pharmacologically delivered and endogenous NO at a distant site have changed the conventional wisdom and introduced NO as an endocrine signalling molecule. The notion is greatly supported by the detection of a number of NO adducts and their circulatory cycles, which in turn contribute to the transport and delivery of NO bioactivity, remote from the sites of its synthesis.The existence of endocrine sites of synthesis, negative feedback regulation of biosynthesis, integrated storage and transport systems, having an exclusive receptor, that is, soluble guanylyl cyclase (sGC), and organized circadian rhythmicity make NO something beyond a simple autocrine/paracrine signalling molecule that could qualify for being an endocrine signalling molecule. Here, we discuss hormonal features of NO from the classical endocrine point of view and review available knowledge supporting NO as a true endocrine hormone. This new insight can provide a new framework within which to reinterpret NO biology and its clinical applications. K E Y W O R D S endocrine, hormone, nitric oxide, signalling molecule

show abstract

“…Of note, another common target of NO and CyPG is the actin cytoskeleton. An increasing number of studies have identified actin and actin-binding proteins (ABPs) as major targets of RNS and CyPG in both physiological and oxidative pathophysiological conditions, resulting in alterations of actin network rearrangements by disturbing filament growth and stabilization (Gayarre et al, 2006;Horenberg et al, 2019;Varland et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Nitric Oxide and Electrophilic Cyclopentenone Prostaglandins in Redox signaling, Regulation of Cytoskeleton Dynamics and Intercellular Communication

Bago

Íñiguez

Serrador

2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Nitric oxide (NO) and electrophilic cyclopentenone prostaglandins (CyPG) are local mediators that modulate cellular response to oxidative stress in different pathophysiological processes. In particular, there is increasing evidence about their functional role during inflammation and immune responses. Although the mechanistic details about their relationship and functional interactions are still far from resolved, NO and CyPG share the ability to promote redox-based post-translational modification (PTM) of proteins that play key roles in cellular homeostasis, signal transduction and transcription. NO-induced S-nitrosylation and S-glutathionylation as well as cyclopentenone-mediated adduct formation, are a few of the main PTMs by which intra- and inter-cellular signaling are regulated. There is a growing body of evidence indicating that actin and actin-binding proteins are susceptible to covalent PTM by these agents. It is well known that the actin cytoskeleton is key for the establishment of interactions among leukocytes, endothelial and muscle cells, enabling cellular activation and migration. In this review we analyze the current knowledge about the actions exerted by NO and CyPG electrophilic lipids on the regulation of actin dynamics and cytoskeleton organization, and discuss some open questions regarding their functional relevance in the regulation of intercellular communication.

show abstract

S‐nitrosylation of cytoskeletal proteins

Cited by 20 publications

References 112 publications

Sex Differences in Biological Processes and Nitrergic Signaling in Mouse Brain

Sex Differences in Biological Processes and Nitrergic Signaling in Mouse Brain

Nitric oxide: To be or not to be an endocrine hormone?

Nitric Oxide and Electrophilic Cyclopentenone Prostaglandins in Redox signaling, Regulation of Cytoskeleton Dynamics and Intercellular Communication

Contact Info

Product

Resources

About