S-nitroso human serum albumin as a nitric oxide donor in drug-eluting vascular grafts: Biofunctionality and preclinical evaluation

Enayati, Marjan; Schneider, Karl H.; Almeria, Ciarra; Grasl, Christian; Kaun, Christoph; Meßner, Barbara; Rohringer, Sabrina; Walter, Ingrid; Wojta, Johann; Budinský, Luboš; Walpoth, Beat H.; Schima, Heinrich; Kager, Gerd; Hallström, Seth; Podesser, Bruno K.; Bergmeister, Helga

doi:10.1016/j.actbio.2021.07.048

Cited by 17 publications

(13 citation statements)

References 44 publications

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“…Therefore, we hypothesized that NO release from nitrate functionalized SDVGs would provide beneficial effects on the promotion of vascular regeneration whilst inhibiting the incidence of graft failure. Results showed that NO improved the regeneration of vascular grafts after 3 months of implantation, consistent with trends observed previously ( Kabirian et al, 2019 ; Enayati et al, 2021 ). Overall, the prolonged, low-level release of NO achieved by PCL/NO SDVGs provided endothelium-mimicry to the graft lumen, whilst regulating VPC regeneration of the EC and VSMC layers, thus achieving markedly improved vascular regeneration, whilst minimizing the occurrence of pathological tissue remodelling.…”

Section: Discussionsupporting

confidence: 91%

“…In physiological tissue microenvironments, NO is a crucial gasotransmitter and signalling molecule, which is continuously produced and released by ECs within the blood vessel lumens ( de Mel et al, 2011 ). Previous studies have focused on developing NO-releasing or NO-generating materials ( Enayati et al, 2021 ). The sustained release of NO was shown to markedly inhibit platelet adhesion and thrombus formation ( Gkaliagkousi et al, 2009 ; Zhao et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

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Nitrate-Functionalized poly(ε-Caprolactone) Small-Diameter Vascular Grafts Enhance Vascular Regeneration via Sustained Release of Nitric Oxide

Yang

Zheng

Qian

et al. 2021

Front. Bioeng. Biotechnol.

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Artificial small-diameter vascular grafts (SDVG) fabricated from synthetic biodegradable polymers, such as poly(ε-caprolactone) (PCL), exhibit beneficial mechanical properties but are often faced with issues impacting their long-term graft success. Nitric oxide (NO) is an important physiological gasotransmitter with multiple roles in orchestrating vascular tissue function and regeneration. We fabricated a functional vascular graft by electrospinning of nitrate-functionalized poly(ε-caprolactone) that could release NO in a sustained manner via stepwise biotransformation in vivo. Nitrate-functionalized SDVG (PCL/NO) maintained patency following abdominal arterial replacement in rats. PCL/NO promoted cell infiltration at 3-months post-transplantation. In contrast, unmodified PCL SDVG showed slow cell in-growth and increased incidence of neointima formation. PCL/NO demonstrated improved endothelial cell (EC) alignment and luminal coverage, and more defined vascular smooth muscle cell (VSMC) layer, compared to unmodified PCL SDVG. In addition, release of NO stimulated Sca-1+ vascular progenitor cells (VPCs) to differentiate and contribute to rapid luminal endothelialization. Furthermore, PCL/NO inhibited the differentiation of VPCs into osteopontin-positive cells, thereby preventing vascular calcification. Overall, PCL/NO demonstrated enhanced cell ingrowth, EC monolayer formation and VSMC layer regeneration; whilst inhibiting calcified plaque formation. Our results suggested that PCL/NO could serve as promising candidates for improved and long-term success of SDVG implants.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Nitrate-Functionalized poly(ε-Caprolactone) Small-Diameter Vascular Grafts Enhance Vascular Regeneration via Sustained Release of Nitric Oxide

Yang

Zheng

Qian

et al. 2021

Front. Bioeng. Biotechnol.

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“…All the studies using stents with either NO-releasing or NO-generating coating show promising results in vitro and in vivo . This is consistent with the results from recent graft study which employed NO-producing vascular graft on rodents for 12 weeks and found rapid endothelialization and hampered SMC proliferation ( Enayati et al, 2021 ). Despite promising outcomes, long-term preclinical evaluations on NO-generating stents are yet to be performed.…”

Section: Novel Developments and Challenges In Stentssupporting

confidence: 92%

Applying Principles of Regenerative Medicine to Vascular Stent Development

Parthiban

Rafuse

Johnson

et al. 2022

Front. Bioeng. Biotechnol.

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Stents are a widely-used device to treat a variety of cardiovascular diseases. The purpose of this review is to explore the application of regenerative medicine principles into current and future stent designs. This review will cover regeneration-relevant approaches emerging in the current research landscape of stent technology. Regenerative stent technologies include surface engineering of stents with cell secretomes, cell-capture coatings, mimics of endothelial products, surface topography, endothelial growth factors or cell-adhesive peptides, as well as design of bioresorable materials for temporary stent support. These technologies are comparatively analyzed in terms of their regenerative effects, therapeutic effects and challenges faced; their benefits and risks are weighed up for suggestions about future stent developments. This review highlights two unique regenerative features of stent technologies: selective regeneration, which is to selectively grow endothelial cells on a stent but inhibit the proliferation and migration of smooth muscle cells, and stent-assisted regeneration of ischemic tissue injury.

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“…After 12 weeks, however, only 40% of the modified grafts revealed no signs of thrombus formation and intimal hyperplasia. In another recent study, Zhang et al 135 utilized the catalytic ability of Cu 2+ to generate nitric oxide from endogenous S-nitrosothiols using copper metal–organic framework (MOF) nanoparticles in electrospun polycaprolactone fibers. After 2 weeks in vivo, the bare PCL grafts were barely covered by endothelial cells and had adhered platelets on the luminal surface.…”

Section: Strategies To Prevent Intimal Hyperplasia In Vascular Graftsmentioning

confidence: 99%

Review of Polymeric Biomimetic Small-Diameter Vascular Grafts to Tackle Intimal Hyperplasia

2022

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Small-diameter artificial vascular grafts (SDAVG) are used to bypass blood flow in arterial occlusive diseases such as coronary heart or peripheral arterial disease. However, SDAVGs are plagued by restenosis after a short while due to thrombosis and the thickening of the neointimal wall known as intimal hyperplasia (IH). The specific causes of IH have not yet been deduced; however, thrombosis formation due to bioincompatibility as well as a mismatch between the biomechanical properties of the SDAVG and the native artery has been attributed to its initiation. The main challenges that have been faced in fabricating SDAVGs are facilitating rapid re-endothelialization of the luminal surface of the SDAVG and replicating the complex viscoelastic behavior of the arteries. Recent strategies to combat IH formation have been mostly based on imitating the natural structure and function of the native artery (biomimicry). Thus, most recently, developed grafts contain a multilayered structure with a designated function for each layer. This paper reviews the current polymeric, biomimetic SDAVGs in preventing the formation of IH. The materials used in fabrication, challenges, and strategies employed to tackle IH are summarized and discussed, and we focus on the multilayered structure of current SDAVGs. Additionally, the future aspects in this area are pointed out for researchers to consider in their endeavor.

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S-nitroso human serum albumin as a nitric oxide donor in drug-eluting vascular grafts: Biofunctionality and preclinical evaluation

Cited by 17 publications

References 44 publications

Nitrate-Functionalized poly(ε-Caprolactone) Small-Diameter Vascular Grafts Enhance Vascular Regeneration via Sustained Release of Nitric Oxide

Nitrate-Functionalized poly(ε-Caprolactone) Small-Diameter Vascular Grafts Enhance Vascular Regeneration via Sustained Release of Nitric Oxide

Applying Principles of Regenerative Medicine to Vascular Stent Development

Review of Polymeric Biomimetic Small-Diameter Vascular Grafts to Tackle Intimal Hyperplasia

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