S-Locus Genotyping on Stone Fruits

Halász, Júlia; Szikriszt, Bernadett; Erċışlı, Sezai; Yılmaz, Kadir; Doğan, Adnan; Szabó, Zoltán; Nyéki, J.; Pedryc, A.; Hegedűs, Attila

doi:10.17660/actahortic.2012.967.12

Cited by 7 publications

(11 citation statements)

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“…The sizes of the PCR products obtained were compared with those previously published by Vilanova et al (2005) and Halász et al (2010) Figure 1. PCR products (in negative) in 63 Turkish apricot accessions using the second intron consensus primers of Prunus S-RNase gene.…”

Section: Resultsmentioning

confidence: 88%

“…Many (22 of 36) of the S-genotypes determined for wild-grown Turkish apricots have also been assigned to Turkish cultivars by Halász et al (2010). Most (9 of 10) S-alleles were also detected in the Turkish apricot cultivars with the exception of S 20 , which is found only in landrace cultivars, not in wild apricots.…”

Section: Discussionmentioning

confidence: 96%

“…Sixty-three seedlings carried 36 different S-genotypes, and 11 combinations of S-alleles have not yet been detected in commercial cultivars (Burgos et al, 1998;Halász et al, 2005Halász et al, , 2007Halász et al, , 2010Lachkar et al, 2013). In such a diverse population, a selection pressure is required to significantly alter allele frequency.…”

Section: Discussionmentioning

confidence: 99%

“…SFB C /SFB 8 -specific primers, AprFBC8-F (5′-CAT GGA AAA AGC TGA CTT ATG G -3′) and AprFBC8-R (5′-GCC TCT AAT GTC ATC TAC TCT TAG -3′), were designed based on the V2 and HVb variable region of the SFB C/8 allele (Halász et al, 2007). The amplification was carried out using a temperature profile, according to Halász et al (2010). PCR was carried out in a PTC 200 thermocycler (MJ Research, Budapest, Hungary).…”

Section: Dna Extractionmentioning

confidence: 99%

“…From Chinese cultivars more than 50 S-alleles have been identified based on S-RNase activity staining, PCR, and sequencing (Jie et al, 2005;Zhang et al, 2008;Wu et al, 2009;Halász et al, 2012). In total, 17 cross-incompatibility groups (CIG) are known, including North American, European, Turkish, and Tunisian apricot cultivars (Szabó and Nyéki, 1991;Egea and Burgos, 1996;Halász et al, 2010;Milatovic et al, 2010;Lachkar et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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The S-genotyping of wild-grown apricots reveals only self-incompatible accessions in the Erzincan region of Turkey

et al. 2013

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IntroductionApricots are grown in warm temperate to subtropical regions of all continents. Over a long period, cultivation moved westward from China to Middle Asia and, further, to Europe. Turkey dominates world apricot production with approximately 500,000 t of annual production. Apricot has been widely cultivated for its edible fruit throughout Turkey since ancient times, especially in Inner Anatolia (e.g., Malatya, Erzincan, Aras valley). Some parts of Turkey, especially the Erzincan plain, have huge native seedling apricot populations. Some of the seedlings genetically reveal late blooming and a dwarf growth habit. These forms may be important for the extension of the harvest period (Ercisli, 2004). In addition, improving the antioxidant capacity of fruits is among the most recent breeding efforts in a range of Prunus species . This can be achieved by including wild accessions into the parental combinations since they accumulate higher levels of antioxidant polyphenolics in fruit compared to popular commercial cultivars (Yildiz et al.

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Section: Resultsmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Dna Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The S-genotyping of wild-grown apricots reveals only self-incompatible accessions in the Erzincan region of Turkey

et al. 2013

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S-locus genotyping on stone fruits in Hungary: a review of the most recent achievements

Halász

2014

Int. j. hortic. sci.

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Central Europe can be taken as a geographical and historical connection zone between the western growing countries and Asian gene centres of Prunus tree fruits. The determination of the S-genotype of stone fruit (mainly almond, plum, cherries and apricot) cultivars and landraces has both practical and theoretical significance. Our group has allocated complete S-genotypes for more than 200 cultivars and selections of almond, Japanese plum, sweet cherry and apricot. Among Eastern European almond cultivars, two novel cross-incompatibility groups (CIGs) were identified. S-alleles of a related species were also shown in P. dulcis accessions; a fact seems to be indicative of introgressive hybridization. Our results with Japanese plum clarified and harmonized two different allele nomenclatures and formed a basis for intensive international studies. In apricot, a total of 13 new S-alleles were identified from Eastern European and Asian accessions. Many Turkish and North African cultivars were classified into new CIGs, III–XVII. Results suggest that the mutation rendering apricot self-compatible might have occurred somewhere in south-east of Turkey and we were successful to confirm the presumed Irano-Caucasian origin of North African apricots based on the geographical distribution of S-alleles. In sweet cherry, new alleles have been identified and characterized from Turkish cultivars and selections. In addition, wild sweet cherry and sour cherry S-alleles were also shown indicating a a broader gene pool in Turkey as compared with international cultivars. We also used S-genotype information of Ukrainian sweet cherry cultivars to design crosses in a functional breeding program. Our results exhibit an increased number of S-alleles in tree fruit accessions native to the regions from Eastern Europe to Central Asia, which can be used to develop S-genotyping methods, to assist cultivation and draw inferences for crop evolution.

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De novo transcriptome assembly and SSR markerdevelopment in apricot (Prunus armeniaca)

Köse¹,

Çetinsağ²,

Gürcan³

2017

Turk J Agric For

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Apricot (Prunus armeniaca) is an important fruit crop worldwide. We have performed a de novo transcriptome assembly for 7 apricot accessions ('Stark Early Orange' (SEO), 'Hacıhaliloğlu' (HH), 'Perfection' , 'Iğdır' , 'Roxana' , 'Esen1' , and 'Esen2'), which yielded a total number of transcripts ranging from 30,363 for 'SEO' to 59,751 for 'Iğdır' . The pool of the reads produced from 7 accessions were assembled into 85,766 transcripts, with an average of 1165.69 nt. Functional annotation (Gene Ontology-GO and Kyoto Encyclopedia of Genes and Genomes-KEGG) was performed successfully for the transcripts. Simple sequence repeats (SSRs) were searched in the transcript pool and 14,722 di-, tri-tetra-, penta-, and hexanucleotide motif loci with a minimum of 5 repetitions for all motifs were identified. Primers were designed for 206 loci, and 72 of them were found to be polymorphic by amplifying diverse 24 apricot accessions, including 7 Plum Pox Virus (PPV)-resistant and 17 PPV-susceptible accessions. In order to test the amplification success of publicly available genomic SSRs (gSSRs) for diverse apricot accessions, an additional 88 published Prunus gSSRs were characterized amplifying the same 24 apricots and only 54 (62%) produced polymorphic bands. The new EST-SSRs could be a reliable source of primers for characterization and mapping studies of apricots, especially because they mostly flank easily scorable tri-and tetranucleotide repeats.

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S-Locus Genotyping on Stone Fruits

Cited by 7 publications

References 0 publications

The S-genotyping of wild-grown apricots reveals only self-incompatible accessions in the Erzincan region of Turkey

The S-genotyping of wild-grown apricots reveals only self-incompatible accessions in the Erzincan region of Turkey

S-locus genotyping on stone fruits in Hungary: a review of the most recent achievements

De novo transcriptome assembly and SSR markerdevelopment in apricot (Prunus armeniaca)

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