S-glutathionylation of fructose-1,6-bisphosphate aldolase confers nitrosative stress tolerance on yeast cells via a metabolic switch

Shino, Seiya; Nasuno, Ryo

doi:10.1016/j.freeradbiomed.2022.10.302

Cited by 2 publications

(2 citation statements)

References 45 publications

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“…In addition to being a proteogenic amino acid, arginine functions as a stress protectant: that is, it acts as an osmolyte, an oxidative stress protectant, a protein-folding chaperone, and a membrane stabilizer ( Baynes et al, 2005 ; Nishimura et al, 2010 ; Mohan et al, 2012 ; Kim et al, 2016 ). Furthermore, nitric oxide synthesized from arginine plays a role in the tolerance to high-temperature and drought as well as in the regulation of sugar metabolism ( Nishimura et al, 2013 ; Nasuno et al, 2014 ; Yoshikawa et al, 2016 ; Shino et al, 2022 ). In short, arginine is essential in maintaining cellular homeostasis by protecting cells from various environmental stresses.…”

Section: Introductionmentioning

confidence: 99%

The arginine transporter Can1 negatively regulates biofilm formation in yeasts

Nishimura,

Tanahashi,

Nakagami

et al. 2024

Front. Microbiol.

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The arginine transporter Can1 is a multifunctional protein of the conventional yeast Saccharomyces cerevisiae. Apart from facilitating arginine uptake, Can1 plays a pivotal role in regulating proline metabolism and maintaining cellular redox balance. Here, we report a novel function of Can1 in the control of yeast biofilm formation. First, the S. cerevisiae CAN1 gene knockout strain displayed a significant growth delay compared to the wild-type strain. Our genetic screening revealed that the slow growth of the CAN1 knockout strain is rescued by a functional deficiency of the FLO8 gene, which encodes the master transcription factor associated with biofilm formation, indicating that Can1 is involved in biofilm formation. Intriguingly, the CAN1 knockout strain promoted the Flo11-dependent aggregation, leading to higher biofilm formation. Furthermore, the CAN1 knockout strain of the pathogenic yeast Candida glabrata exhibited slower growth and higher biofilm formation, similar to S. cerevisiae. More importantly, the C. glabrata CAN1 gene knockout strain showed severe toxicity to macrophage-like cells and nematodes. The present results could help to elucidate both the molecular mechanism underlying yeast biofilm formation and the role it plays. Future investigations may offer insights that contribute to development of antibiofilm agents.

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Section: Introductionmentioning

confidence: 99%

The arginine transporter Can1 negatively regulates biofilm formation in yeasts

Nishimura,

Tanahashi,

Nakagami

et al. 2024

Front. Microbiol.

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“…It means the ux of PPP can be mediated by regulating the glycolytic pathway. When the glycolysis is inhibited, the ow of glucose to the PPP increases [7], thereby promoting the increase of NADPH, which is the reducing power for fatty acids synthesis [8]. Shino carried out nitrosation stress in Saccharomyces cerevisiae, and the activity of aldolase was inhibited, which led to the transformation of glycolysis pathway to PPP, thereby increasing the intracellular NADPH content and enhancing the tolerance of yeast to nitrosation stress [9].…”

Section: Introductionmentioning

confidence: 99%

Functions of aldolase in lipid synthesis of Schizochytrium sp. by gene disruption to switch carbon metabolism

Zhang,

Wu,

Guo

et al. 2024

Preprint

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Background As a key rate-limiting enzyme in the glycolytic pathway of cells, aldolase affects the distribution of intracellular carbon flux and determines the overall ability of subsequent cell metabolism, which are mainly reported in the medical related researches, but rarely involved microorganisms. In this study, the aldolase gene of Schizochytrium limacinum SR21 (ALDOA) was knocked out to explore the effect of regulating carbon flux on cell growth and lipid synthesis. Results The knockout of ALDOA showed an adverse effect on cell growth and total lipids production, which was decreased by 9.6% and 23.2%, respectively, but helped to improve the synthetic ability of polyunsaturated fatty acids (PUFAs), in which the proportion of docosahexaenoic acid (DHA) increased by 22.9%. Analysis of phospholipomics, real-time quantitative PCR and metabolomics revealed that the knockout of ALDOA weakened the glycolysis pathway and tricarboxylic acid cycle to inhibit cell growth, and lowered the Kennedy pathway to reduce the production of total lipids and the synthesis of phospholipids to affect cell metabolism. Correspondingly, the knockout of ALDOA enhanced the metabolic flux of the pentose phosphate pathway to provide more reducing power for PUFAs accumulation and improved the glycerophosphorylcholine acylation pathway to promote the accumulation of DHA. Conclusions ALDOA knockout redistributes the carbon metabolic flux in cells, by weakening the glycolysis, tricarboxylic acid cycle and glyceride synthesis pathway to inhibit cell growth and total lipid production, and strengthening the pentose phosphate pathway and glycerophosphorylcholine acylation pathway to increase the synthesis of PUFAs and DHA accumulation. This study provides a new idea for identifying the aldolase function in microorganisms and a metabolic strategy to improve DHA accumulation in Schizochytrium.

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S-glutathionylation of fructose-1,6-bisphosphate aldolase confers nitrosative stress tolerance on yeast cells via a metabolic switch

Cited by 2 publications

References 45 publications

The arginine transporter Can1 negatively regulates biofilm formation in yeasts

The arginine transporter Can1 negatively regulates biofilm formation in yeasts

Functions of aldolase in lipid synthesis of Schizochytrium sp. by gene disruption to switch carbon metabolism

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