S-Allele Characterization in Self-Incompatible Pear (Pyrus Communis): Biochemical, Molecular and Field Analyses

Zuccherelli, S.; Broothaerts, W.; Tassinari, Paola; Tartarini, Stefano; Dondini, Luca; Bester, A J; Sansavini, Silviero

doi:10.17660/actahortic.2002.596.18

Cited by 6 publications

(4 citation statements)

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“…Although very close genetic similarities exist among the members of the Rosaceae family, it was possible to characterize ten European pear cultivars for their S-genotypes using specific sequences of apple S-alleles. The molecular analysis evinced similarities and differences among the cultivars, confirming previous partial results obtained via a biochemical approach (Zuccherelli et al 2002). The comparison of both molecular and biochemical methods is important as additional confirmation of results, and for those species in which the investigation of S-alleles has just begun.…”

Section: Discussionsupporting

confidence: 80%

“…Inter-compatibility among European pear (Pyrus communis L.) cultivars has been studied mainly through field observations (Bellini 1993) and biochemical analyses (Tomimoto et al 1996;Zuccherelli et al 2002). The present study was undertaken to determine compatibility among European pear cultivars via a molecular PCRbased method (S-PCR) optimized with primers specifically designed for apple.…”

Section: Introductionmentioning

confidence: 99%

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S-Allele characterization in self-incompatible pear (Pyrus communis L.)

Zuccherelli

Tassinari

Broothaerts

et al. 2002

Sexual Plant Reproduction

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Gametophytic self-incompatibility, a natural mechanism occurring in pear and other fruit-tree species, is usually controlled by the S-locus with allelic variants (S1, S2, Sn). Recently, biochemical and molecular tools have determined the S-genotype of cultivars in various species. The present study determined the S-locus composition of ten European pear cultivars via S-PCR molecular assay, thereby obviating time-consuming fieldwork whose results are often ambiguous because of environmental effects. To verify the S-PCR assay, two putative S-allele DNA fragments of Japanese pear were isolated; their sequences proved to be identical to those reported in the databank. Six S-allele fragments of European pear were then sequenced. While field data confirmed the molecular results, fully and half-compatible field crosses were not distinguishable.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

S-Allele characterization in self-incompatible pear (Pyrus communis L.)

Zuccherelli

Tassinari

Broothaerts

et al. 2002

Sexual Plant Reproduction

View full text Add to dashboard Cite

show abstract

“…Also, often it is affected by many other factors such as environmental and physiological parameters. It should be noted that it is complex to distinguish between fully compatible and semi compatible crosses (Zuccherelli et al 2002). Recently, using of molecular methods for determination of S-genotype, where many of them are based on polymorphisms of the S-RNase gene, is increased (Sanzol and Robbins 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Genomic PCRs with consensus primers, designed from nucleotide sequences of apple S-RNases, were used to amplify six putative S-RNase alleles in pears (Zuccherelli et al 2002). Zisovich et al (2004) identified seven S-alleles in European pear cultivars by PCR assay and sequencing, also expressed compatibility relations between nine pear cultivars.…”

Section: Introductionmentioning

confidence: 99%