Rytmonorm-Induced Cytogenetic and Testicular Damage in Albino Rats: The Protective Effect of Grapefruit Juice

Abstract

The present work studied the cytogenetic and testicular damage induced by the antiarrhythmic drug rytmonorm (RT) and the protective role of grapefruit juice in albino rats. Treating rats with RT (13.5mg/Kg bodyweight) for five weeks caused a significant increase in the percentage of chromosomal aberrations either structural or numerical and decrease the mitotic index. Using comet assays, RT caused DNA fragmentation in the lymphocytes. Testis of RT treated rats showed many histopathological and morphometric alterations. In addition, the number of sperm head abnormalities was increased. Treating animals with RT and grapefruit juice (27ml/Kg bodyweight) led to an improvement in chromosomal aberrations, mitotic index, DNA damage, and testicular alterations caused by RT. In conclusion, the results of this study indicated that grapefruit juice showed a protective effect on cytotoxicty and testicular alterations induced by RT in albino rats and this is may be due to its potent antioxidant effects.

The Protective Impact of Vitamin E against Atenolol Effect on Reproductive Efficiency in Male Rats

“…Our results show atenolol treatment did not affect the gonadosomatic index (GSI) significantly and this results reinforced with (Lin et al, 2015). A significant decrease in sperm aviability was detected in group T1 after 56 days of treatment with atenolol may be due to oxidative insult which effect on sperm DNA and male fertility and could be effect on spermatogenisis and steroid biosynthesis of Leydig cells (Sakr et al, 2013) or may be due to the effect of atenolol on concentration of testosterone (Khan et al, 2004) promote spermatogenesis and maintenance of sperm production (Pineda et al, 2003). This may be attributed to the antioxidant effect of vitamin E which is responsible for its protective effect against oxidative stress, so spermatozoa are protected by antioxidant properties of vitamin E, which removed free radical and improve the sperm viability and gonadosomatic index (GSI) (Momeni et al, 2009).…”

“…Other study (Sakr et al, 2013) showed that animal given rytmonorm for five weeks showed an increase in the mean number of structural chromosomal aberration, also antihypertensive drug atenolol was found to induce chromosom loss. In addition animal group that treated with atenolol plus vitamin E shows there is less sperm DNA damage due to that vitamin E ameliorate the side effect of atenolol on sperm DNA and allows free radicals to abstract a hydrogen atom from the antioxidant molecule rather than from polyunsaturated fatty acids, thus breaking the chain of free radical reactions, the resulting antioxidant radicals being a relatively unreactive species (Aldana et al, 2001).…”

“…Different clinical studies show that level of fragmentation of the chromatin above30% is related with spontaneous abortion or no pregnancy. (Carrell et al, 2003) Moreover antihypertensive agent atenolol was found out to induce chromosomal aberration and sexual dysfunction, impotence (Manolis et al, 2012) oxidative stress and sperm DNA damage (Rocco et al, 2012) However, the prolonged administration of atenolol causes reduction in serum glutathione (GSH) concentration because it has minimal effects on angiotensin II production in the body through the inhibition of rennin angiotensin system which acting an essentially to diminishing GSH (Haidar et al, 2014) which has influencing sperm DNA integrity to produce chromosomal abnormality and thus influence sperm parameters (Sakr et al, 2013). The stimulatory effect of catecholamine's on lipolysis is mediated by beta receptor stimulating property.…”

Effect of Grapefruit Juice on Aluminum-Induced Hepatotoxicity in Albino Rats: Histological, Ultrastructural and Histochemical Assessment

“…Group II: Rats were orally administered with grapefruit juice (Citrus paradise) at a dose level of 27 ml/kg body weight daily for 6 weeks [14]. Grapefruit (Citrus paradise) used in the work was purchased from local markets in Jeddah…”

“…[14] reported that grapefruit prevents cytogenetic and testicular damage induced by rytmonorm in rats. The objective of this work is to study the effect of grapefruit in aluminum hepatotoxicity in albino rats.…”