RYBP-PRC1 Complexes Mediate H2A Ubiquitylation at Polycomb Target Sites Independently of PRC2 and H3K27me3

Tavares, Lígia; Dimitrova, Emiliya; Oxley, David; Webster, Judith; Poot, Raymond A.; Demmers, Jeroen; Bezstarosti, Karel; Taylor, Stuart A.; Ura, Hiroki; Koide, Hiroshi; Wutz, Anton; Vidal, Miguel; Elderkin, Sarah; Brockdorff, Neil

doi:10.1016/j.cell.2012.06.011

Cited by 125 publications

(191 citation statements)

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“…Interestingly, we also observed that: 1) BMI1 did not co-purify with EZH2 or H3K27 me3 ; 2) EZH2 and H3K27 me3 were not enriched at constitutive heterochromatin; and 3) BMI1 accumulation at constitutive heterochromatin and HOXC13 was EZH2 independent. Although apparently surprising, these results are in agreement with numerous findings showing that PRC1 recruitment can be PRC2 independent, or that the PRC1 can work upstream of the PRC2 (29,61,62). It is also notable that RING1B knockdown could not mimic the BMI1-deficient phenotype.…”

Section: Discussionsupporting

confidence: 80%

The Polycomb Repressive Complex 1 Protein BMI1 Is Required for Constitutive Heterochromatin Formation and Silencing in Mammalian Somatic Cells

Abdouh

Hanna

Hajjar

et al. 2016

Journal of Biological Chemistry

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Background: BMI1 silences the expression of genes located at the facultative heterochromatin. Results: BMI1 is abundant at repetitive genomic regions, including the pericentromeric heterochromatin (PCH), where it is required for compaction and silencing. Conclusion: BMI1 is essential for PCH formation. Significance: BMI1 function at PCH is important to understand how BMI1 regulates genomic stability.

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Section: Discussionsupporting

confidence: 80%

The Polycomb Repressive Complex 1 Protein BMI1 Is Required for Constitutive Heterochromatin Formation and Silencing in Mammalian Somatic Cells

Abdouh

Hanna

Hajjar

et al. 2016

Journal of Biological Chemistry

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“…7, G-J, with levels of binding plotted as the enrichment p value. To validate the presence of the entire PRC1 complex in cluster L1, we also analyzed the genome-wide binding of CBX7 recently reported by the Brockdorff group (12). A comparison of the normalized average enrichment for gene clusters L1-4 is shown in supplemental Fig.…”

Section: Tbp Binding Is Retained At Ring1b-bound Promoters In Mouse Ementioning

confidence: 99%

Polycomb Repressive Complex 1 (PRC1) Disassembles RNA Polymerase II Preinitiation Complexes

Lehmann

Ferrari

Vashisht

et al. 2012

Journal of Biological Chemistry

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“…Immunoprecipitations were carried out as described (Tavares et al 2012;Latos et al 2015) and are detailed in the Supplemental Material. DNA from four immunoprecipitations was pooled for each library generated with the NEB Next DNA library preparation master mix (New England Biolabs, E6040) according to the manufacturer's instructions.…”

Section: Chip-seqmentioning

confidence: 99%

Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks

et al. 2015

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Elf5 is a transcription factor with pivotal roles in the trophoblast compartment, where it reinforces a trophoblast stem cell (TSC)-specific transcriptional circuit. However, Elf5 is also present in differentiating trophoblast cells that have ceased to express other TSC genes such as Cdx2 and Eomes. In the present study, we aimed to elucidate the context-dependent role of Elf5 at the interface between TSC self-renewal and the onset of differentiation. We demonstrate that precise levels of Elf5 are critical for normal expansion of the TSC compartment and embryonic survival, as Elf5 overexpression triggers precocious trophoblast differentiation. Through integration of protein interactome, transcriptome, and genome-wide chromatin immunoprecipitation data, we reveal that this abundancedependent function is mediated through a shift in preferred Elf5-binding partners; in TSCs, Elf5 interaction with Eomes recruits Tfap2c to triply occupied sites at TSC-specific genes, driving their expression. In contrast, the Elf5 and Tfap2c interaction becomes predominant as their protein levels increase. This triggers binding to double-and single-occupancy sites that harbor the cognate Tfap2c motif, causing activation of the associated differentiationpromoting genes. These data place Elf5 at the center of a stoichiometry-sensitive transcriptional network, where it acts as a molecular switch governing the balance between TSC proliferation and differentiation.

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RYBP-PRC1 Complexes Mediate H2A Ubiquitylation at Polycomb Target Sites Independently of PRC2 and H3K27me3

Cited by 125 publications

References 0 publications

The Polycomb Repressive Complex 1 Protein BMI1 Is Required for Constitutive Heterochromatin Formation and Silencing in Mammalian Somatic Cells

The Polycomb Repressive Complex 1 Protein BMI1 Is Required for Constitutive Heterochromatin Formation and Silencing in Mammalian Somatic Cells

Polycomb Repressive Complex 1 (PRC1) Disassembles RNA Polymerase II Preinitiation Complexes

Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks

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