Ryanodine receptor calcium release channels in trophoblasts and their role in cell migration

Zheng, Limian; Lindsay, Andrew J.; McSweeney, Kate; Aplin, John D.; Forbes, Karen; Smith, Samantha D.; Tunwell, Richard; Mackrill, John J.

doi:10.1016/j.bbamcr.2021.119139

Cited by 4 publications

(2 citation statements)

References 56 publications

(84 reference statements)

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“…Vitamin D regulates cytoskeletal reorganization in other tissues (62,63). In trophoblasts, vitamin D treatment promotes cellular migration and invasion in vitro, processes that are dependent on cytoskeletal reorganization (11,12,64,65). Cytoskeletal proteins and intracellular membrane trafficking pathways are also necessary for placental nutrient transport, including trafficking amino acid and glucose transporters, as well as fusion into the multinucleate syncytium (66,67).…”

Section: Discussionmentioning

confidence: 99%

Associations Between Prenatal Vitamin D and Placental Gene Expression

Parenti,

Melough,

Lapehn

et al. 2024

Preprint

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Background: Vitamin D is a hormone regulating gene transcription. Prenatal vitamin D has been linked to immune and vascular function in the placenta, a key organ of pregnancy. To date, studies of vitamin D and placental gene expression have focused on a limited number of candidate genes. Transcriptome-wide RNA sequencing can provide a more complete representation of the placental effects of vitamin D. Objective: We investigated the association between prenatal vitamin D levels and placental gene expression in a large, prospective pregnancy cohort. Methods: Participants were recruited in Shelby County, Tennessee in the Conditions Affecting Neurocognitive Development and Learning in Early childhood (CANDLE) study. Vitamin D level (plasma total 25-hydroxyvitatmin D, [25(OH)D]) was measured at mid-pregnancy (16-28 weeks' gestation) and delivery. Placenta samples were collected at birth. RNA was isolated and sequenced. We identified differentially expressed genes (DEGs) using adjusted linear regression models. We also conducted weighted gene co-expression network analysis (WGCNA). Results: The median 25(OH)D of participants was 21.8 ng/mL at mid-pregnancy (N=774, IQR: 15.4-26.5 ng/mL) and 23.6 ng/mL at delivery (N=753, IQR: 16.8-29.1 ng/mL). Placental expression of 25 DEGs was associated with 25(OH)D at mid-pregnancy, but no DEG was associated with 25(OH)D at delivery. DEGs were related to energy metabolism, cytoskeletal function, and RNA transcription. Using WGCNA, we identified 2 gene modules whose expression was associated with 25(OH)D at mid-pregnancy and 1 module associated with 25(OH)D at delivery. These modules were enriched for genes related to mitochondrial and cytoskeletal function, and were regulated by transcription factors includingARNT2,BHLHE40,FOSL2,JUND, andNFKB1. Conclusions: Our results indicate that 25(OH)D during mid-pregnancy, but not at delivery, is associated with placental gene expression at birth. Future research is needed to investigate a potential role of vitamin D in programming placental mitochondrial metabolism, intracellular transport, and transcriptional regulation during pregnancy.

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Section: Discussionmentioning

confidence: 99%

Associations Between Prenatal Vitamin D and Placental Gene Expression

Parenti,

Melough,

Lapehn

et al. 2024

Preprint

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“…Polymerization of F-actin in vibrio splendidus agglutinated by a subunit of oligomerization domain-like receptors (NLRs) is Ca 2+ -dependent 37 . Similarly, cytoplasmic Ca 2+ regulates the reorganization of F-actin in BeWo cell line 38 . We propose that stimulation of 5-HT 2B Rs triggers Ca 2+ signals that interact with F-actin to dynamically control the shape and volume of ependymocytes.…”

Section: Ependymocytes Regulate Csf Movementmentioning

confidence: 97%

Ependymocytes control cerebrospinal fluid flow to the peripheral organs through periaxonal pathway

Li,

Wang,

Zhang

et al. 2023

Preprint

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SummaryMechanisms controlling movement of the CSF through the central canal towards the peripheral nerves are poorly characterized. We found that fluorescent dyes injected into cisterna magna are carried with cerebrospinal fluid (CSF) through the central canal and peripheral nerves to the peripheral organs such as liver, and pancreas. We also found close connection between spinal axons and ependymocytes, suggesting synaptic interactions. Serotonin, acting through the 5-HT2Breceptors abundantly expressed in ependymal cells, trigger Ca2+signal that induces polymerization of cytoskeleton protein F-actin, consequently reducing the volume of ependymocytes. Shrinkage of the latter opens one-way route to facilitate CSF outflow from the central canal into the spinal cord parenchyma and peripheral nerves. In liver, CSF is received by stellate cells. Ependymal control over transfer of the CSF from central canal to peripheral organs by the periaxonal space (PAS) represents a novel mechanism dynamically connecting the CNS with the periphery.In briefEpendymocytes control CSF flow from CNS to peripheral organs by periaxonal pathway, and serotonin evokes the ependymal shrinkage by the aggregation of F-actin.HighlightsEpendymocytes control CSF flow from central canal to peripheral organs; CSF flows through the peripheral periaxonal space to reach the peripheral organs; Serotonin makes ependymocytes shrunk by 5-HT2Breceptor-mediated Ca2+signaling and F-actin polymerization; The hepatic stellate cells are potential collectors of CSF in the liver.

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