Ryanodine-affinity chromatography purifies 106 kD Ca2+ release channels from skeletal and cardiac sarcoplasmic reticulum

Salama, Guy; Nigam, Meenakshi; Shome, Kuntala; Finkel, Mitchell S.; Lagenaur, Carl F.; Zaidi, Nikhat F.

doi:10.1016/0143-4160(92)90074-3

Cited by 12 publications

(3 citation statements)

References 31 publications

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“…It is likely that there are many regulatory sites localized on this N-terminal region of those receptors, such as a phosphorylation site and calcium binding site. At present, it is known that these two channels are regulated by phosphorylation (Nakada et al, 1994) and redox state (Salama et al, 1992), as well as by cytoplasmic regulating proteins, such as ankyrin (Bourguognon et al, 1993) and FK 506-binding protein (FKBP; a 12-kDa protein that has immunosuppresive activity and has recently been identified as an inhibitor of protein kinase C [Collins, 1991;Brillantes et al, 194;Cameron et al, 1995]). It has also been suggested that calcium channels may be regulated by endoplasmic reticulum (ER) during oocyte maturation.…”

Section: Discussionmentioning

confidence: 97%

Dynamic Changes to the Inositol 1,4,5-Trisphosphate and Ryanodine Receptors during Maturation of Bovine Oocytes

Wang

White

Reed

et al. 2005

Cloning and Stem Cells

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The inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) and ryanodine receptor (RyR) have been identified as two ligand-gated calcium channels which play a critical role in mediating calcium release in many different types of cells and tissues. The physiological significance of the two receptors in regulation of intracellular calcium during meiotic maturation and fertilization in the bovine oocyte was evaluated. Metabolic labeling of bovine oocytes by Met-Cys 35S during early and late maturation was followed by immunoprecipitation of both RyR and IP3R using specific antibodies against these two receptors. Results indicate that IP3R is translated throughout the maturation period; in contrast, RyR is only translated during the late maturation period of bovine oocytes. In addition, the experiments reported here investigate the temporal and spatial relationships between these calcium channels and the endoplasmic reticulum (ER) and cortical granules (CG). Immunocytochemistry, fluorescence staining and confocal microscopy were applied at four oocyte developmental stages: the germinal vesicleintact (GV-intact), metaphase I (MI) and metaphase II (MII) stages of maturation and the fertilized egg at 6 h post insemination (hpi). Although oocytes demonstrated some differences in staining patterns and localization, both receptor types showed apparent dynamic changes during meiotic maturation and dramatic decreases in signals after insemination. These results indicate the changes in the number and distribution of IP3R and RyR may account for the increased intracellular calcium responsiveness at fertilization. The IP3R appears to associate with the ER at the sub-vitelline membrane cortex in bovine oocytes. In addition, RyR appears to associate with the CG. In conclusion, although these two receptors may have different functional roles in regulation of calcium release during meiotic maturation and fertilization, it appears that both IP3R and RyR contribute to the significant increase of intracellular calcium during fertilization and activation in the bovine oocyte.

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Section: Discussionmentioning

confidence: 97%

Dynamic Changes to the Inositol 1,4,5-Trisphosphate and Ryanodine Receptors during Maturation of Bovine Oocytes

Wang

White

Reed

et al. 2005

Cloning and Stem Cells

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“…Salama and colleagues have shown that Ca 2+ release from the sarcoplasmic reticulum is triggered by application of Cu 2 +/cysteine, reactive disulfides, or heavy metals [67]. This response, however, may be due to an effect on a 106-kDa protein that may be the ryanodine receptor [68]. Inhibition of thimerosal-induced Ca 2 + release by heparin and the antibody to the IP 3 receptor indicates that thimerosal modifies the IP 3 receptor in mouse and hamster eggs.…”

Section: Response To Injection Of Ip 3 or Addition Of Thimerosalmentioning

confidence: 93%

Regulation of Intracellular Calcium in the Mouse Egg: Evidence for Inositol Trisphosphate-Induced Calcium Release, but not Calcium-Induced Calcium Release1

Kline

1994

Biology of Reproduction

115

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Fertilization of the mammalian egg initiates transient and repetitive release of Ca2+ from intracellular stores. The mechanism by which these Ca2+ transients are produced is not completely known. We examined the role of two principal Ca2+ release mechanisms, inositol trisphosphate-induced Ca2+ release and Ca(2+)-induced Ca2+ release, in altering intracellular Ca2+ in the mouse egg. Microinjection of inositol 1,4,5-trisphosphate (IP3) transiently elevated intracellular Ca2+ and, at higher concentrations, produced repetitive Ca2+ transients. Addition of 100 microM thimerosal, a sulfhydryl reagent, caused repetitive Ca2+ transients. IP3 and thimerosal responses were inhibited by prior injection of heparin, a competitive antagonist of IP3-induced Ca2+ release. Addition of caffeine or injection of caffeine, ryanodine, or cyclic ADP-ribose, which are known to initiate or modulate Ca(2+)-induced Ca2+ release in sea urchin eggs and other cells, produced no change in intracellular Ca2+. The response to injection of Ca2+ was not altered by prior injection of ryanodine. The magnitude of the Ca2+ transients produced by injection of IP3 was not changed by prior injection of cyclic ADP-ribose or external caffeine. We found no evidence of Ca(2+)-induced Ca2+ release from ryanodine- or caffeine-sensitive stores. It is most likely that release of Ca2+ from intracellular stores in the mouse egg is dependent on IP3-induced Ca2+ release.

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“…Ca 2+ channels are known to be regulated by phosphorylation (Nakade et al, 1994) and changes in redox state (Salama et al, 1992) as well as by interacting with cytoplasmic proteins such as ankyrin (Bourgguignon et al, 1993) and FK506-binding protein (Brillantes, 1994;Cameron et al, 1995). Changes in the inherent properties of Ca 2+ channels is a likely possibility.…”

Section: Development Of Ca 2+ Release Mechanisms During Oogenesismentioning

confidence: 99%

Ca2+ release and the development of Ca2+ release mechanisms during oocyte maturation: a prelude to fertilization

Carroll

Jones²,

Whittingham³

1996

Reviews of Reproduction

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Oogenesis involves the production of an oocyte that can undergo fertilization and support early development. The stimulus that initiates embryogenesis is an increase in the concentration of intracellular Ca2+ in the cytoplasm of the oocyte at the time of fertilization. The development of the ability of the oocyte to release Ca2+ in response to the fertilizing spermatozoon is an essential step in the process of oogenesis. Mammalian oocytes are particularly useful for studying the development of Ca2+ signalling systems, owing to the series of Ca2+ oscillations generated at fertilization, compared with the monotonic Ca2+ increase seen in nonmammalian species. Recent evidence has revealed that Ca2+ release mechanisms are modified during oogenesis. The maximal sensitivity of Ca2+ release is reached in the final stages of oocyte maturation, just before the optimal time for fertilization. In this review, we consider the mechanism underlying Ca2+ release in mammalian oocytes and discuss how the release mechanisms are modified during oocyte maturation. The tight co-ordination of the differentiation of the Ca2+ signalling system with the development of the oocyte provides a means of ensuring successful activation at the time of fertilization. Finally, we consider the consequences for embryo development in circumstances in which the co-ordination is lost.

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Ryanodine-affinity chromatography purifies 106 kD Ca2+ release channels from skeletal and cardiac sarcoplasmic reticulum

Cited by 12 publications

References 31 publications

Dynamic Changes to the Inositol 1,4,5-Trisphosphate and Ryanodine Receptors during Maturation of Bovine Oocytes

Dynamic Changes to the Inositol 1,4,5-Trisphosphate and Ryanodine Receptors during Maturation of Bovine Oocytes

Regulation of Intracellular Calcium in the Mouse Egg: Evidence for Inositol Trisphosphate-Induced Calcium Release, but not Calcium-Induced Calcium Release1

Ca2+ release and the development of Ca2+ release mechanisms during oocyte maturation: a prelude to fertilization

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