Ryanodine Activates Sea Urchin Eggs

Sardet, Christian; Gillot, I.; Ruscher, A.; Payan, P.; Girard, Jean‐Pierre; Renzis, Guy De

doi:10.1111/j.1440-169x.1992.00037.x

Cited by 28 publications

(2 citation statements)

References 41 publications

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“…2B). This spontaneous release might occur through RyR (16); caffeine and ryanodine caused Ca2+ release in sea urchin eggs (7,17) and in homogenates (7), whereas ruthenium red blocked caffeineinduced Ca2+ release. Spontaneous release after addition of Ca2+ was detected in the presence of ruthenium red (Fig.…”

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confidence: 99%

“…The similarity between ligand binding to surface receptors and the sperm-egg interaction during fertilization has led to the proposal that Ca2+ mobilization associated with fertilization is similarly mediated by IP3. Indeed, phosphoinositide metabolism increased during fertilization coincident with the increase in the concentration of intracellular Ca2+ ([Ca2'+i) and the cortical exocytotic reaction (16,17 We investigated the effects of heparin on the IP3-induced Ca2+ mobilization in intact sea urchin eggs and homogenates. In egg homogenates, Ca2+ release induced by IP3 (1 p.M) was completely blocked by heparin (0.2 mg/ml) ( Fig.…”

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Redundant Mechanisms of Calcium-Induced Calcium Release Underlying Calcium Waves During Fertilization of Sea Urchin Eggs

Galione

McDougall

Busa

et al. 1993

Science

303

202

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Propagating Ca2+ waves are a characteristic feature of Ca(2+)-linked signal transduction pathways. Intracellular Ca2+ waves are formed by regenerative stimulation of Ca2+ release from intracellular stores by Ca2+ itself. Mechanisms that rely on either inositol trisphosphate or ryanodine receptor channels have been proposed to account for Ca2+ waves in various cell types. Both channel types contributed to the Ca2+ wave during fertilization of sea urchin eggs. Alternative mechanisms of Ca2+ release imply redundancy but may also allow for modulation and diversity in the generation of Ca2+ waves.

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confidence: 99%

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confidence: 99%

Redundant Mechanisms of Calcium-Induced Calcium Release Underlying Calcium Waves During Fertilization of Sea Urchin Eggs

Galione

McDougall

Busa

et al. 1993

Science

303

202

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The Two Intracellular Ca2+Release Channels, Ryanodine Receptor and Inositol 1,4,5-Trisphosphate Receptor, Play Different Roles during Fertilization in Ascidians

Albrieux¹,

Sardet

Villaz³

1997

Developmental Biology

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Fertilization in the ascidians triggers an activation wave of calcium release followed by intracellular calcium oscillations synchronous with periodic membrane potential excursions during the completion of the meiotic cell cycle. Fertilization also causes a fast decrease in the egg plasma membrane depolarization-activated calcium current and a large increase in capacitance thought to represent membrane addition to the egg surface. We have analyzed the temporal and causal relationships between these changes in the eggs of Phallusia mammillata using whole-cell patch-clamp recording while simultaneously imaging calcium with fura-2 dextran. We have defined the role of ryanodine receptor (RyR) and InsP3 receptor (InsP3R) during fertilization and meiosis by looking at the effects of InsP3, cyclic ADP ribose (cADPR), and ryanodine in perfused oocytes. We show that InsP3 (10 microM perfused through the patch pipette) is able to trigger sustained oscillations in intracellular calcium concentration in unfertilized oocytes, resembling those recorded in fertilized egg completing meiosis. In addition the sustained oscillations resulting from InsP3 perfusion in unfertilized oocytes are sufficient to cause the emission of both polar bodies. In contrast, ryanodine or cADPR never trigger detectable calcium signal in perfused oocytes. Instead, nanomolar concentrations of ryanodine or cADPR cause a capacitance change, implying a net insertion of membrane to the oocyte surface, and trigger a fast decrease in the depolarization-activated calcium current. Both changes are similar to the changes in conductance and capacitance naturally observed following fertilization. These effects, although not associated with measurable calcium signals, are abolished by coperfusion of the calcium chelator BAPTA. In contrast to ryanodine or cADPR, sustained perfusion of the oocyte with nanomolar concentrations of InsP3 causes no capacitance change and a slow and moderate decrease in calcium current. Our observations on inseminated patch-clamped eggs further indicate that membrane insertion, which starts 15-20 sec after the onset of the membrane conductance change at fertilization, can be altered by interfering with the RyR. Our results imply that, in ascidians, as in some mammals, RyR and InsP3R play distinct roles during fertilization.

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Comparative Biology of Calcium Signaling during Fertilization and Egg Activation in Animals

Stricker

1999

Developmental Biology

665

563

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During animal fertilizations, each oocyte or egg must produce a proper intracellular calcium signal for development to proceed normally. As a supplement to recent synopses of fertilization-induced calcium responses in mammals, this paper reviews the spatiotemporal properties of calcium signaling during fertilization and egg activation in marine invertebrates and compares these patterns with what has been reported for other animals. Based on the current database, fertilization causes most oocytes or eggs to generate multiple wavelike calcium oscillations that arise at least in part from the release of internal calcium stores sensitive to inositol 1,4,5-trisphosphate (IP3). Such calcium waves are modulated by upstream pathways involving oolemmal receptors and/or soluble sperm factors and in turn regulate calcium-sensitive targets required for subsequent development. Both "protostome" animals (e.g., mollusks, annelids, and arthropods) and "deuterostomes" (e.g., echinoderms and chordates) display fertilization-induced calcium waves, IP3-mediated calcium signaling, and the ability to use a combination of external calcium influx and internal calcium release. Such findings fail to support the dichotomy in calcium signaling modes that had previously been proposed for protostomes vs deuterostomes and instead suggest that various features of fertilization-induced calcium signals are widely shared throughout the animal kingdom.

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Ryanodine Activates Sea Urchin Eggs

Cited by 28 publications

References 41 publications

Redundant Mechanisms of Calcium-Induced Calcium Release Underlying Calcium Waves During Fertilization of Sea Urchin Eggs

Redundant Mechanisms of Calcium-Induced Calcium Release Underlying Calcium Waves During Fertilization of Sea Urchin Eggs

The Two Intracellular Ca2+Release Channels, Ryanodine Receptor and Inositol 1,4,5-Trisphosphate Receptor, Play Different Roles during Fertilization in Ascidians

Comparative Biology of Calcium Signaling during Fertilization and Egg Activation in Animals

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