RxpsG a new open project for Photoelectron and Electron Spectroscopy data processing

Speranza, G.; Canteri, R.

doi:10.1016/j.softx.2019.100282

Cited by 43 publications

(31 citation statements)

References 21 publications

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“…The main core lines acquired were O 1s, C 1s, N 1s, S 2p and Au 4f. The relative elemental percentage, that gives a semiquantitative analysis, was obtained by integrating the area under the core lines, by applying the Shirley background subtraction, and by correcting for the atomic sensitivity factors through a dedicated software [ 42 ]. Using the attenuation of the Au 4f 7/2 signal between the different samples it was possible to calculate the overlayer thickness (d OL ) through the following equation:

where I 0 Au is the Au 4f 7/2 peak intensity on the clean gold sample, I Au is the Au 4f 7/2 peak intensity of the gold sample after treatment, and λ OL is the attenuation length of Au 4f 7/2 photoelectrons in the polymer overlayer (3.45 nm) [ 43 ].…”

Section: Methodsmentioning

confidence: 99%

SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor

Cennamo

Pasquardini²,

Arcadio

et al. 2021

Talanta

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A specific aptameric sequence has been immobilized on short polyethyleneglycol (PEG) interface on gold nano-film deposited on a D-shaped plastic optical fiber (POFs) probe, and the protein binding has been monitored exploiting the very sensitive surface plasmon resonance (SPR) phenomenon. The receptor-binding domain (RBD) of the SARS-CoV-2 spike glycoprotein has been specifically used to develop an aptasensor. Surface analysis techniques coupled to fluorescence microscopy and plasmonic analysis have been utilized to characterize the biointerface. Spanning a wide protein range (25÷1000 nM), the SARS-Cov-2 spike protein was detected with a Limit of Detection (LoD) of about 37 nM. Different interferents (BSA, AH1N1 hemagglutinin protein and MERS spike protein) have been tested confirming the specificity of our aptasensor. Finally, a preliminary test in diluted human serum encouraged its application in a point-of-care device, since POF-based aptasensor represent a potentially low-cost compact biosensor, characterized by a rapid response, a small size and could be an ideal laboratory portable diagnostic tool.

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Section: Methodsmentioning

confidence: 99%

SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor

Cennamo

Pasquardini²,

Arcadio

et al. 2021

Talanta

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“…The quantification, reported as relative elemental percentage, was carried out using the core levels and the atomic sensitivity factors. All XPS data were analyzed using the software described in Speranza and Canteri [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Nanostructured SmFeO3 Gas Sensors: Investigation of the Gas Sensing Performance Reproducibility for Colorectal Cancer Screening

Gaiardo

Zonta

Gherardi

et al. 2020

Sensors

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Among the various chemoresistive gas sensing properties studied so far, the sensing response reproducibility, i.e., the capability to reproduce a device with the same sensing performance, has been poorly investigated. However, the reproducibility of the gas sensing performance is of fundamental importance for the employment of these devices in on-field applications, and to demonstrate the reliability of the process development. This sensor property became crucial for the preparation of medical diagnostic tools, in which the use of specific chemoresistive gas sensors along with a dedicated algorithm can be used for screening diseases. In this work, the reproducibility of SmFeO3 perovskite-based gas sensors has been investigated. A set of four SmFeO3 devices, obtained from the same screen-printing deposition, have been tested in laboratory with both controlled concentrations of CO and biological fecal samples. The fecal samples tested were employed in the clinical validation protocol of a prototype for non-invasive colorectal cancer prescreening. Sensors showed a high reproducibility degree, with an error lower than 2% of the response value for the test with CO and lower than 6% for fecal samples. Finally, the reproducibility of the SmFeO3 sensor response and recovery times for fecal samples was also evaluated.

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“…The individual core lines were acquired at high resolution by setting the analyzer passing energy at 20 eV and the energy step at 0.05 eV. The spectra were analyzed using homemade software based on the R platform [19]. Peak fitting was performed using linear background subtraction and Gaussian components.…”

Section: Characterizationsmentioning

confidence: 99%

Facile Synthesis of Water-Soluble, Highly-Fluorescent Graphene Quantum Dots from Graphene Oxide Reduction for Efficient Cell Labelling

Minati¹,

Piano²

2019

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In this work, we report a simple, one-step, green procedure to fabricate strong blue and yellow photoluminescent graphene quantum dots (GQDs) as by-product of the synthesis of mesoporous graphene hydrogel (GHs). The graphene hydrogel was obtained by chemical reduction of graphene oxide using ascorbic acid at mild temperature. As a consequence of the network formation, small fluorescent GQDs can be isolated from the residual solvent, purified from the by-products and finally concentrated to produce GQDs. The GQDs chemistry and morphology were characterized by X-ray Photoelectron Spectroscopy (XPS) and Transmission Electron Microscopy (TEM). The GQDs mean diameter was about 5–10 nm and they exhibited an intense luminescence in the visible range with an excitation wavelength-dependent fluorescence. Our experiments showed that GQDs were easily internalized in living cells and furthermore, such internalization did not adversely affect cell viability.

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RxpsG a new open project for Photoelectron and Electron Spectroscopy data processing

Cited by 43 publications

References 21 publications

SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor

SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor

Nanostructured SmFeO3 Gas Sensors: Investigation of the Gas Sensing Performance Reproducibility for Colorectal Cancer Screening

Facile Synthesis of Water-Soluble, Highly-Fluorescent Graphene Quantum Dots from Graphene Oxide Reduction for Efficient Cell Labelling

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