Rv3303c of Mycobacterium tuberculosis protects tubercle bacilli against oxidative stress in vivo and contributes to virulence in mice

Akhtar, Parvez; Srivastava, Shubhra; Srivastava, A. K.; Srivastava, Mukesh; Srivastava, Bhavana; Srivastava, Ranjana

doi:10.1016/j.micinf.2006.09.004

Cited by 32 publications

(27 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…M. tuberculosis also secretes superoxide dismutase encoded by sodA (24) which may protect against ROI, although conclusive data on this function are lacking in M. tuberculosis. Various cell wall components (25,26), reductases (27,28), and the genes responsible for mycothiol production (29) also appear to play a protective role. Lsr2 appears to use a different mechanism to protect mycobacteria against oxidative stress that requires direct protein-DNA binding.…”

Section: Discussionmentioning

confidence: 99%

The multifunctional histone-like protein Lsr2 protects mycobacteria against reactive oxygen intermediates

Colangeli

Haq

Arcus

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

120

112

View full text Add to dashboard Cite

Mycobacterium tuberculosis has evolved a number of strategies to survive within the hostile environment of host phagocytes. Reactive nitrogen and oxygen intermediates (RNI and ROI) are among the most effective antimycobacterial molecules generated by the host during infection. Lsr2 is a M. tuberculosis protein with histonelike features, including the ability to regulate a variety of transcriptional responses in mycobacteria. Here we demonstrate that Lsr2 protects mycobacteria against ROI in vitro and during macrophage infection. Furthermore, using macrophages derived from NOS ؊/؊ and Phox ؊/؊ mice, we demonstrate that Lsr2 is important in protecting against ROI but not RNI. The protection provided by Lsr2 protein is not the result of its ability to either bind iron or scavenge hydroxyl radicals. Instead, electron microscopy and DNAbinding studies suggest that Lsr2 shields DNA from reactive intermediates by binding bacterial DNA and physically protecting it. Thus, Lsr2 appears to be a unique protein with both histone-like properties and protective features that may be central to M. tuberculosis pathogenesis. In addition, evidence indicates that lsr2 is an essential gene in M. tuberculosis. Because of its essentiality, Lsr2 may represent an excellent candidate as a drug target.Mycobacterium tuberculosis ͉ ROI ͉ DPS ͉ DNA

show abstract

Section: Discussionmentioning

confidence: 99%

The multifunctional histone-like protein Lsr2 protects mycobacteria against reactive oxygen intermediates

Colangeli

Haq

Arcus

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

120

112

View full text Add to dashboard Cite

show abstract

“…Previous studies have shown that some ivi genes are related to bacterial pathogenesis (Deb et al 2002;Kim et al 2003;Fernández et al 2004;Akhtar et al 2006;Lee et al 2007). To confirm these findings, three ivi genes identified in this study were evaluated for their roles in virulence of V. anguillarum.…”

Section: Discussionmentioning

confidence: 99%

“…Among these technologies, IVIAT is significantly superior to other reported in vivo expression technologies because it avoids the use of animal model (Handfield et al 2005). It has been applied in several important pathogenic bacteria for the identification of new virulent factors (Deb et al 2002;Kim et al 2003;Fernández et al 2004;Akhtar et al 2006;Lee et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Screening of genes expressed in vivo after infection by Vibrio anguillarum M3

Zou

Hao

et al. 2010

Letters in Applied Microbiology

View full text Add to dashboard Cite

Aims: Genes uniquely expressed in vivo may contribute to the overall pathogenicity of an organism and are likely to serve as potential targets for the development of new vaccine. This study aims to screen the genes expressed in vivo after Vibrio anguillarum infection by in vivo‐induced antigen technology (IVIAT). Methods and Results: The convalescent‐phase sera were obtained from turbot (Scophthalmus maximus) survived after infection by the virulent V. anguillarum M3. The pooled sera were thoroughly adsorbed with M3 cells and Escherichia coli BL21 (DE3) cells. A genomic expression library of M3 was constructed and screened for the identification of immunogenic proteins by colony immunoblot analysis with the adsorbed sera. After three rounds of screening, 19 putative in vivo‐induced (ivi) genes were obtained. These ivi genes were catalogued into four functional groups: regulator/signalling, metabolism, biological process and hypothetical proteins. Three ivi genes were insertion‐mutated, and the growth and 50% lethal dose (LD50) of these mutants were evaluated. Conclusions: The identification of ivi genes in V. anguillarum M3 sheds light on understanding the bacterial pathogenesis and provides novel targets for the development of new vaccines and diagnostic reagents. Significance and Impact of the Study: To the best of our knowledge, this is the first report describing in vivo‐expressed genes of V. anguillarum using IVIAT. The screened ivi genes in this study could be new virulent factors and targets for the development of vaccine, which may have implications for the development of diagnostic regents.

show abstract

“…Some published articles have demonstrated that quinones are active against Mycobacterium tuberculosis, Mycobacterium smegmatis and Mycobacterium avium. The mechanism of these compounds is still being investigated, although some reports have suggested that quinones stimulate oxidative stress in biological systems (Tran et al 2004, Akhtar et al 2006, Silva et al 2008.…”

mentioning

confidence: 99%

In vitro synergic effect of ²-lapachone and isoniazid on the growth of Mycobacterium fortuitum and Mycobacterium smegmatis

Silva

Mesquita

Ximenes

2009

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Rv3303c of Mycobacterium tuberculosis protects tubercle bacilli against oxidative stress in vivo and contributes to virulence in mice

Cited by 32 publications

References 32 publications

The multifunctional histone-like protein Lsr2 protects mycobacteria against reactive oxygen intermediates

The multifunctional histone-like protein Lsr2 protects mycobacteria against reactive oxygen intermediates

Screening of genes expressed in vivo after infection by Vibrio anguillarum M3

In vitro synergic effect of ²-lapachone and isoniazid on the growth of Mycobacterium fortuitum and Mycobacterium smegmatis

Contact Info

Product

Resources

About