Rutin Suppresses DMBA Carcinogenesis in the Breast Through Modulating IL-6/NF-κB, SRC1/HSP90 and ER-α

Abstract

Rutin dietary supplements may offer pharmacological benefits as anticancer and antiinflammatory properties. This study aimed to investigate the inhibitory and protective effect of rutin on signaling pathways of mammary gland carcinogenesis expermintally induced in female rats by 7,12-di-methyl benz (a) anthracene (DMBA). Results showed that rutin administration ameliorated DMBA toxicity and carcinogic effect on kidney and liver revealed by a significant decrease of urea and creatinine levels, and the activity of the liver enzymes alanine aminotransferase (ALT) and alkaline phosphatase (ALP). The antioxidant state indicated by the total antioxidant capacity (TAC) was significantly increased accompanied by a reduction in the inflammatory markers of interleukin-1β (IL-1B), interleukin-6 (IL-6), and tumor necrosis factor (TNF-α) with induction of apoptosis indicated by a significant increase in caspase-3 level. Rutin significantly reduced the levels of the tumor markers carcinoma antigen 15-3 (CA 15-3) and proto-oncogene tyrosine-protein kinase Src1 (Src1). along with downregulation of nuclear factor-kB (NF-κB), heat shock protein 90 (HSP 90), and inducible nitric oxide synthase (iNOS) gene expression. The present study demonstrated the beneficial anticancer activity of rutin as a protective and therapeutic agent. Rutin induces its antitumor activity through elevation of the antioxidant state, inhibition of inflammatory cytokines, downregulation of oncogenes expression, and stimulation of apoptosis.

Sıçanlarda Valproik Asite Bağlı Testis Hasarına Rutin Etkisinin Araştırılması

“…Inflammation occurs in response to a chemical, physical or biological substance in the body (Ambriz-Pérez et al, 2016). TNF-α, IL-1β and MAPK14 are proinflammatory cytokines (Gur, Kandemir, et al, 2022;Temel et al, 2020) Previous studies have shown that RUT inhibits TNF-α and IL-1β levels in various tissues (Cihan et al, 2022;Kandemir, Caglayan, et al, 2020;Youssef et al, 2022). In the presented study, it was determined that there were significant increases in TNF-α, IL-1β and MAPK14 mRNA transcript levels in testicular tissue triggered by VALP application to rats.…”

The effect of Phyllanthus niruriand Catharanthus roseuson Macrophage Polarization in Breast Cancer Mice Model

“…The carcinogenicity of DMBA is known to be due to its mechanisms involved in DNA damage. Genes that play an essential role in cell growth and survival are damaged, causing mutations and an imbalance between cellular oxidants and antioxidants, which play an essential role in breast cancer development and progression [16].…”

Effect of Ambrosia arborescens Mill. ethanolic extract on breast cancer induced in rats

“…Histopathological analysis in the control group that received the carcinogen DMBA showed a histological pattern of ductal carcinoma characterized by Available at www.veterinaryworld.org/Vol.17/March-2024/22.pdf malignancy criteria [28]. According to the American Society of Cancer, this histological change (ductal cancers) corresponds to the most frequent type of breast cancer in women [29].…”