Rutin Promotes Pancreatic Cancer Cell Apoptosis by Upregulating miRNA-877-3p Expression

Abstract

(1) Background: pancreatic cancer is one of the most serious cancers due to its rapid and inevitable fatality, which has been proved very difficult to treat, compared with many other common cancers. Thus, developing an effective therapeutic strategy, especially searching for potential drugs, is the focus of current research. The exact mechanism of rutin in pancreatic cancer remains unknown. (2) Method: three pancreatic cancer cell lines were used to study the anti-pancreatic cancer effect of rutin. The potent anti-proliferative, anti-migration and pro-apoptotic properties of rutin were uncovered by cell viability, a wound-healing migration assay, and a cell apoptosis assay. High-throughput sequencing technology was used to detect the change of miRNAs expression. Immunoblotting analysis was used to detect the expression of apoptotic proteins. (3) Results: CCK-8 and EDU assays revealed that rutin significantly inhibited pancreatic cancer cells’ proliferation (p < 0.05). A wound-healing assay showed that rutin significantly suppressed pancreatic cancer cells’ migration (p < 0.05). A flow cytometric assay showed that rutin could promote pancreatic cancer cells’ apoptosis. Intriguingly, rutin significantly upregulated miR-877-3p expression to repress the transcription of Bcl-2 and to induce pancreatic cancer cell apoptosis. Accordingly, rutin and miR-877-3p mimics could promote apoptotic protein expression. (4) Conclusions: our findings indicate that rutin plays an important role in anti-pancreatic cancer effects through a rutin-miR-877-3p-Bcl-2 axis and suggests a potential therapeutic strategy for pancreatic cancer.

Prevention of polycystic ovary syndrome and postmenopausal osteoporosis by inhibiting apoptosis with Shenling Baizhu powder compound

“…Previous studies have shown that Rutin can reduce vancomycin-induced renal tubular apoptosis by inhibiting apoptosis, reducing intracellular reactive oxygen species, and improving mitochondrial dysfunction ( Qu et al, 2019 ). Rutin upregulates miR-877-3p expression, inhibits Bcl-2 transcription and induces apoptosis in pancreatic cancer cells ( Huo et al, 2022 ). Rutin also inhibits autophagy and Akt activation-mediated apoptosis and attenuates adriamycin-induced cardiotoxicity ( Ma et al, 2017 ).…”

Rutin alleviates colon lesions and regulates gut microbiota in diabetic mice

“…The rutin used in this study was obtained by ethanol extraction from tartary buckwheat powder, the purity of rutin prepared by us reached more than 96% and stored in our laboratory as previously described 27 .…”

Role of bio‐flavonols and their derivatives in improving mitochondrial dysfunctions associated with pancreatic tumorigenesis

“…Supress proliferation, increase apoptosis via upregulating miR-877-3p in Panc-1, SW-1990, and MiaPaCa-2 cells Huo et al 119 Exerts antioxidant action and increases apoptosis in combination with orlistat Saleh et al 120 Abbreviations: EGF, epidermal growth factor; GSK, glycogen synthase kinase; mTOR, mammalian target of rapamycin; NF-κB, nuclear factor kappa B; PARP, poly ADP ribose polymerase; ROS, reactive oxygen species; STAT3, signal transducer and activator of transcription 3; TGF, transforming growth factor; VEGF, vascular endothelial growth factor.…”

“…237 In a recent study, three different pancreatic carcinoma cell lines (Panc-1, SW1990, and MiaPaCa-2) treated with rutin (isolated from tartary buckwheat) showed suppressed proliferation and increased apoptosis via upregulating the miRNA-877-3p. 119 Furthermore, the anticancer property of rutin in combination with orlistat was tested against Panc-1 cells and the other two breast cancer models (EAC and MCF-7). Their results showed that the combinatorial treatment of both drugs exerted antioxidant action and apoptosis in tumor cells, signifying rutin's potential for future anticancer therapies.…”

“…Rutin significantly decreased the expression of inflammatory pathway genes (IκB kinase‐α/β, NF‐κB) in colon cancer (HT29 cells) 237 . In a recent study, three different pancreatic carcinoma cell lines (Panc‐1, SW1990, and MiaPaCa‐2) treated with rutin (isolated from tartary buckwheat) showed suppressed proliferation and increased apoptosis via upregulating the miRNA‐877‐3p 119 . Furthermore, the anticancer property of rutin in combination with orlistat was tested against Panc‐1 cells and the other two breast cancer models (EAC and MCF‐7).…”