Rutin loaded liquid crystalline nanoparticles inhibit non-small cell lung cancer proliferation and migration in vitro

Paudel, Keshav Raj; Wadhwa, Ridhima; Tew, Xin Nee; Lau, Natalie Jia Xin; Madheswaran, Thiagarajan; Panneerselvam, Jithendra; Zeeshan, Farrukh; Kumar, Pradeep; Gupta, Gaurav; Anand, Krishnan; Singh, Sachin Kumar; Jha, Niraj Kumar; MacLoughlin, Ronan; Hansbro, Nicole G.; Liu, Gang; Shukla, Shakti D.; Mehta, Meenu; Hansbro, Philip M.; Chellappan, Dinesh Kumar; Dua, Kamal

doi:10.1016/j.lfs.2021.119436

Cited by 67 publications

(28 citation statements)

References 49 publications

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“…The physicochemical characterization/parameters and in vitro drug release of berberine LCNs were found in agreement with our previous studies involving similar LCNs encapsulating other phytoceuticals, rutin, and naringenin. Moreover, we have also observed significant in vitro anticancer activity with those LCNs formulation (Paudel et al., 2021; Wadhwa et al., 2021). This overlay of the findings amongst various phytoceuticals berberine, rutin and naringenin clearly highlights the validity and application of LCNs in lung cancer.…”

Section: Discussionmentioning

confidence: 52%

Berberine loaded liquid crystalline nanostructure inhibits cancer progression in adenocarcinomic human alveolar basal epithelial cells in vitro

et al. 2021

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Metastasis represents the leading cause of death in lung cancer patients. C‐X‐C Motif Chemokine Ligand 8 (CXCL‐8), Chemokine (C‐C motif) ligand 20 (CCL‐20) and heme oxygenase −1 (HO‐1) play an important role in cancer cell proliferation and migration. Berberine is an isoquinoline alkaloid isolated from several herbs in the Papaveraceae family that exhibits anti‐inflammatory, anticancer and antidiabetic properties. Therefore, the aim of present study is to investigate the inhibitory potential of berberine monoolein loaded liquid crystalline nanoparticles (berberine‐LCNs) against cancer progression. Berberine‐LCNs were prepared by mixing berberine, monoolein and poloxamer 407 (P407) using ultrasonication method. A549 cells were treated with or without 5 µM dose of berberine LCNs for 24 hr and total cellular protein was extracted and further analyzed for the protein expression of CCl‐20, CXCL‐8 and HO‐1 using human oncology array kit. Our results showed that berberine‐LCNs significantly reduced the expression of CCl‐20, CXCL‐8 and HO‐1 at dose of 5µM. Collectively, our findings suggest that berberine‐LCNs have inhibitory effect on inflammation/oxidative stress related cytokines i.e. CCL20, CXCL‐8, and HO‐1 which could be a novel therapeutic target for the management of lung cancer. Practical applications Berberine is an isoquinoline alkaloid extracted from various plants of Papaveraceae family. CXCL‐8, CCL‐20 and HO‐1 play an important role in cancer progression. Our study showed that Berberine LCNs significantly downregulate the expression of CXCL‐8, CCL‐20 and HO‐1 which suggests that Berberine loaded nanoparticles could be a promising therapeutic alternative for the management of lung cancer.

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Section: Discussionmentioning

confidence: 52%

Berberine loaded liquid crystalline nanostructure inhibits cancer progression in adenocarcinomic human alveolar basal epithelial cells in vitro

et al. 2021

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“…The inherent molecular interactions existing within the nanosystems were extracted and analysed using atomistic simulations in vacuum through a conjugate gradient within an MM + force field as described elsewhere (ChemLite 30 Molecular Modelling Software) (Fig. 1) (Chan et al 2020;Paudel et al 2021).…”

Section: Preparation Of Berberine-lcnsmentioning

confidence: 99%

“…Among nanoformulations, liquid crystalline nanoparticles (LCNs) have gained a lot of interest in the pharmaceutical sector because of their extraordinary potential to improve medication bioavailability and enhanced stability of the active drug component. In addition, these nanostructures have the inherent ability to alter the release of drug when administered through various routes (Paudel et al 2021). Monoolein (MO) is a type of lipid used in the formulation of LCNs.…”

Section: Introductionmentioning

confidence: 99%

Berberine-loaded liquid crystalline nanoparticles inhibit non-small cell lung cancer proliferation and migration in vitro

Paudel

Mehta

Yin

et al. 2022

Environ Sci Pollut Res

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Non-small cell lung cancer (NSCLC) is reported to have a high incidence rate and is one of the most prevalent types of cancer contributing towards 85% of all incidences of lung cancer. Berberine is an isoquinoline alkaloid which offers a broad range of therapeutical and pharmacological actions against cancer. However, extremely low water solubility and poor oral bioavailability have largely restricted its therapeutic applications. To overcome these limitations, we formulated berberine-loaded liquid crystalline nanoparticles (LCNs) and investigated their in vitro antiproliferative and antimigratory activity in human lung epithelial cancer cell line (A549). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), trypan blue staining, and colony forming assays were used to evaluate the anti-proliferative activity, while scratch wound healing assay and a modified Boyden chamber assay were carried out to determine the anti-migratory activity. We also investigated major proteins associated with lung cancer progression. The developed nanoparticles were found to have an average particle size of 181.3 nm with spherical shape, high entrapment efficiency (75.35%) and have shown sustained release behaviour. The most remarkable findings reported with berberine-loaded LCNs were significant suppression of proliferation, inhibition of colony formation, inhibition of invasion or migration via epithelial mesenchymal transition, and proliferation related proteins associated with cancer progression. Our findings suggest that anti-cancer compounds with the problem of poor solubility and bioavailability can be overcome by formulating them into nanotechnology-based delivery systems for better efficacy. Further in-depth investigations into anti-cancer mechanistic research will expand and strengthen the current findings of berberine-LCNs as a potential NSCLC treatment option.

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“…Rutin is a naturally occurring bioflavonoid abundantly reported in medicinal herbs, fruits, vegetables, and plant-based beverages. Research has further presented the in vitro potential of rutin in the suppression of numerous human cancers such as lung cancer, prostate cancer, colorectal cancer, breast cancer, liver cancer, glioblastoma, melanoma, osteosarcoma, ovarian cancer, leukemia, cervical cancer, and pancreatic cancer via apoptosis induction, immunity enhancement, or cell migration knockdown, which leads to a significant reduction in the motility rate of cancerous cells [20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Rutin (Bioflavonoid) as Cell Signaling Pathway Modulator: Prospects in Treatment and Chemoprevention

et al. 2021

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Cancer is a complex ailment orchestrated by numerous intrinsic and extrinsic pathways. Recent research has displayed a deep interest in developing plant-based cancer therapeutics for better management of the disease and limited side effects. A wide range of plant-derived compounds have been reported for their anticancer potential in the quest of finding an effective therapeutic approach. Rutin (vitamin P) is a low-molecular weight flavonoid glycoside (polyphenolic compound), abundantly present in various vegetables, fruits (especially berries and citrus fruits), and medicinal herbs. Numerous studies have delineated several pharmacological properties of rutin such as its antiprotozoal, antibacterial, anti-inflammatory, antitumor, antiviral, antiallergic, vasoactive, cytoprotective, antispasmodic, hypolipidemic, antihypertensive, and antiplatelet properties. Specifically, rutin-mediated anticancerous activities have been reported in several cancerous cell lines, but the most common scientific evidence, encompassing several molecular processes and interactions, including apoptosis pathway regulation, aberrant cell signaling pathways, and oncogenic genes, has not been thoroughly studied. In this direction, we attempted to project rutin-mediated oncogenic pathway regulation in various carcinomas. Additionally, we also incorporated advanced research that has uncovered the notable potential of rutin in the modulation of several key cellular functions via interaction with mRNAs, with major emphasis on elucidating direct miRNA targets of rutin as well as the process needed to transform these approaches for developing novel therapeutic interventions for the treatment of several cancers.

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Rutin loaded liquid crystalline nanoparticles inhibit non-small cell lung cancer proliferation and migration in vitro

Cited by 67 publications

References 49 publications

Berberine loaded liquid crystalline nanostructure inhibits cancer progression in adenocarcinomic human alveolar basal epithelial cells in vitro

Berberine loaded liquid crystalline nanostructure inhibits cancer progression in adenocarcinomic human alveolar basal epithelial cells in vitro

Berberine-loaded liquid crystalline nanoparticles inhibit non-small cell lung cancer proliferation and migration in vitro

Rutin (Bioflavonoid) as Cell Signaling Pathway Modulator: Prospects in Treatment and Chemoprevention

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