Rutin inhibits the proliferation of murine leukemia WEHI-3 cells in vivo and promotes immune response in vivo

Lin, Jing; Yang, Jai Sing; Lu, Chi Cheng; Chiang, Jen‐Huai; Wu, Chang Lin; Lin, Jen Jyh; Lin, Hui Lu; Yang, Mei; Liu, Kuo Ching; Chiu, Te-Fa; Chung, Jing Gung

doi:10.1016/j.leukres.2008.09.032

Cited by 85 publications

(59 citation statements)

References 13 publications

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“…The antitumor and toxicity-reducing effects of HJT extract are potentially associated with components such as flavonoids, xanthones, glucoside and embelin in HJT, which also contribute to the immune activity on the tumor cells (15)(16)(17)(18). However, in the present study, the whole herb extract was used, while the concrete mechanism of action remains to be elucidated.…”

Section: Discussionmentioning

confidence: 88%

The effect-enhancing and toxicity-reducing activity of Hypericum japonicum Thunb. extract in murine liver cancer chemotherapy

Lü

Cao

Zhen-hua

et al. 2012

Molecular and Clinical Oncology

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Abstract. Chinese herbs are potential sources of antitumor drugs with immunoregulatory activity and few adverse effects. In the present study, we investigated whether the Hypericum japonicum Thunb. (HJT) extract enhanced the efficacy of 5-fluorouracil (5-FU) treatment in murine liver tumor xenografts and reduced toxicity of chemotherapy in hepatoma H22-bearing mice. Tumor weight and inhibition rate, thymus and spleen indices, as well as white blood cell (WBC) count were calculated. The phagocytic function of macrophages was assessed by observing peritoneal macrophages phagocytized chicken red blood cells (RBC). Body weight and toxic reactions of the chemotherapeutic and life prolongation rate were investigated in the mice. Results demonstrated that the HJT extract significantly enhanced the tumor inhibition rate of 5-FU, improved the immune function, reduced the toxic effects and prolonged the survival time in the tumor-bearing mice. Taken together, these results indicated that the HJT extract has a synergistic tumor-inhibiting effect with 5-FU, is able to reduce the toxic side effects and is likely to be safe and efficacious for use in antitumor therapy. IntrodutionLiver cancer is a malignant disease with a high incidence and mortality, and is often diagnosed at an advanced stage (1). Common therapies, such as surgical resection, percutaneous or transarterial interventions are of limited efficacy (2), as chemotherapy, one of the main measures for a comprehensive treatment, may induce adverse effects such as bone marrow suppression, gastrointestinal reactions and lowered immunological function.Several Chinese herbs have been identified as potential sources of antitumor drugs (3-5). The effect-enhancing and toxicity-reducing activity of Chinese herbs in radiotherapy and chemotherapy of tumors was previously confirmed (6,7). However, no studies focusing on the antitumor and toxicity-reducing effects of Hypericum japonicum Thunb. (HJT) have been reported thus far. Therefore, the present investigation was conducted to ascertain whether the HJT extract enhanced the efficacy of 5-fluorouracil (5-FU) treatment in murine liver tumor xenografts, and reduced toxicity of chemotherapy in tumor-bearing mice. Materials and methodsReagents. RPMI-1640 medium, fetal bovine serum (FBS), penicillin G and streptomycin were obtained from Invitrogen Corp. (Carlsbad, CA, USA). 5-FU was obtained from the Shanghai Xudong Haipu Pharmaceutical Co., Ltd. (Shanghai, China). Murine H22 hepatoma cells were purchased from the Shanghai Institute of Cell Biology at the Chinese Academy of Sciences (Shanghai, China). Animals. Institute of Cancer Research (ICR) mice(male/female, 50-50%; weight, 20±2 g) were purchased from the Experimental Animal Center, Zhengzhou University (Zhengzhou, China). The animals were kept in a standard laboratory, under a 12-h light/dark cycle, at room temperature (20±2˚C), in a humid (75±15%) and filtered laminar air flow-controlled room in the animal facility of the Experimental Animal Center of the Zhengzhou University...

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Section: Discussionmentioning

confidence: 88%

The effect-enhancing and toxicity-reducing activity of Hypericum japonicum Thunb. extract in murine liver cancer chemotherapy

Lü

Cao

Zhen-hua

et al. 2012

Molecular and Clinical Oncology

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“…Cucurbitacin B (cucB), a triterpenoid from Cucurbitaceae vegetables also found in bitter melon seeds, caused cell cycle arrest and apoptosis induction in human colon adenocarcinoma cancer cells [53]. Additionally, Rutin, a flavonoid present in bitter melon leaves, has been reported to display growth inhibition of leukemia and ovarian carcinoma cells, with anti-invasive effects on melanoma cells [35,[56][57][58]. Therefore, BMLE may include other bioactive compounds apart from kuguacin J, which exert anti-tumor effects, although human studies have not yet been published.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, 2 flavonoids and 4 phenolic acids, including rutin, naringin, gentistic acid, benzoic acid, ocoumaric acid, and t-cinnamic acid, are present in bitter melon leaves [35]. Rutin, a flavonoid glycoside, has been reported to successfully show growth inhibition of leukemia and ovarian carcinomas, with anti-invasive effects on melanoma [35,[56][57][58]. Triterpenoids and flavoniods included in bitter melon leaf extract (BMLE) might be promising components with critical roles against cancer cell progression, but the active compound(s) remain to be identified.…”

Section: Phytochemicals and Cucurbitane Triterpenoidsmentioning

confidence: 99%

Kuguacin J, a Triterpenoid from Momordica charantia Linn: A Comprehensive Review of Anticarcinogenic Properties

Limtrakul¹,

Pitchakarn²,

Suzuki³

2013

Carcinogenesis

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“…Retinoids are potent agents for controlling cellular differentiation and proliferation and often used for hemopoietic malignancies therapy in vivo [16]. Other clinical signs of leukemia, including splenomegaly and hepatomegaly in the control leukemia mice [17], were mitigated by the extract treatment, evidenced by the significant reduction in the spleens and livers towards the sizes and weights of normal mice. The aggressive WEHI-3B leukemia cells had invaded the blood forming organs including the spleen and liver of the leukemia-induced mice.…”

Section: Discussionmentioning

confidence: 99%

Morinda Citrifolia Leaf Extract Ameliorated Leukemia in Mice Model

Ahmadi

et al. 2017

JABB

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Leukemia affects over a million people worldwide and disturbs the bone marrow, the spleen and the lymph system. In finding dietary therapy against leukemia the vegetable Morinda citrifolia leaves were investigated and compared with ATRA (all-trans-retinoic acid) in mice. The extract standardised to epicatechin and scopoletin was anti-proliferative against human JURKAT and murine WEHI-3B leukemia cells (IC 50 of 14.5 and 17μg /ml respectively) in vitro yet was nontoxic to normal peripheral blood mononuclear cells. Leukemia was induced in the BALB/c mice by inoculating WEHI-3B cells subcutaneously. Three days after leukemia development the extract (100 and 200mg /Kg BW) or ATRA (5mg/ kg BW) were fed daily to the mice for four weeks. The extract decreased the leukemic mice peripheral blood immature leukocytes, anemia, total white blood cells; bone marrow blasts cells content and blast cells invasions to the spleen and liver. Electron microscopy and histopathology observations confirmed the extract induced leukemia cells apoptosis similar to ATRA. The extract also mitigated the changes in T-cells and B-cells levels in the leukemic spleen tissues in vivo. The extract dose dependently induced leukemia cells apoptosis both in vitro and in vivo restored all pathological blood, liver and spleen changes in vivo and reinstated the endogenous immune responses to normal healthy levels in vivo. [7] and Typhonium flagelliforme [8]. Morinda citrifolia (Noni) or `mengkudu' in Malaysia has been extensively used for its broad therapeutic effects, including various anticancer activities, in both clinical practice and laboratory animal models [9]. The Morinda citrifolia leaves are often eaten as vegetables after blanching by the locals of South East Asia. The leaves are also traditionally used medicinally to treat wound infections, pain, arthritis, swellings and for general health. The leaves have liver-protective, antioxidant and skin healing effects [10]. The leaves are scientifically reported to have no acute, sub-acute or sub-chronic oral toxicity [11]. The oral no observed-adverseeffect level (NOAEL) of M. citrifolia leaves ethanolic extract is 1000 mg/kg [12]. This work demonstrates the therapeutic effects of the edible Morinda citrifolia (Mengkudu/Noni) leaf extract for leukemia and the possible modes of action in mice model. Keywords Materials and Methods Plant materialsM. citrifolia leaves (Voucher No. SK2322/14, identified by Biodiversity Unit, Institute of Bioscience, University Putra Malaysia, Serdang) were collected from Institute Bioscience, Morinda Citrifolia Leaf Extract Ameliorated Leukemia in Mice Model 2/11Copyright: ©2017 Ahmadi et al. University Putra Malaysia, State of Selangor, Malaysia. The leaves were dried, coarsely ground and soaked overnight in 50% aqueous ethanol in the leaf: solvent ratio (w/v) of 1:5 (kg/l), with occasional stirring. The extract was filtered and rotary evaporated to remove all solvents. The yield was 13.6%. The leaf extract is standardized using HPLC (Waters 2996, Milford, MA)...

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Rutin inhibits the proliferation of murine leukemia WEHI-3 cells in vivo and promotes immune response in vivo

Cited by 85 publications

References 13 publications

The effect-enhancing and toxicity-reducing activity of Hypericum japonicum Thunb. extract in murine liver cancer chemotherapy

The effect-enhancing and toxicity-reducing activity of Hypericum japonicum Thunb. extract in murine liver cancer chemotherapy

Kuguacin J, a Triterpenoid from Momordica charantia Linn: A Comprehensive Review of Anticarcinogenic Properties

Morinda Citrifolia Leaf Extract Ameliorated Leukemia in Mice Model

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