Rutin improves glutamate uptake and inhibits glutamate excitotoxicity in rat brain slices

Ferreira, Rafael Short; Teles-Souza, Jéssica; Souza, Cleide dos Santos; Pereira, Érica Patrícia Lima; Araújo, F.; Silva, Alessandra Bispo da; Silva, Juliana Helena Castro E; Nonose, Yasmine; Nuñez-Figueredo, Yanier; Assis, Adriano Martimbianco de; Souza, Diogo Onofre de; Costa, Maria de Fátima Dias; Moreira, José Cláudio Fonseca; Costa, Sílvia Lima; Silva, Victor D. A. da

doi:10.1007/s11033-021-06145-y

Cited by 11 publications

(16 citation statements)

References 46 publications

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“…Rutin might have potential for the treatment of ethanol-induced hepatotoxicity and exhibit a beneficial effect on regulating mitochondrial functions [ 31 , 32 ]. Moreover, rutin could be a neuroprotective agent, which improved the glutamate metabolism of brains in rats and prevented glutamate excitotoxicity [ 33 ]. In addition, ellagic acid could ameliorate features of metabolic syndrome [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from Akebia trifoliata Peels

Luo

Zhou

Shang

et al. 2021

Foods

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Akebia trifoliata is a fruit with rich nutritional properties, and its peel is produced as a by-product. In this research, we investigated the influences of microwave-assisted extraction parameters on antioxidant activity of the extract from Akebia trifoliata peels, and the ferric-reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) as well as total phenolic contents (TPC) were used to optimize extraction parameters. The influences of ethanol concentration, microwave power and solvent-to-material ratio, as well as extraction temperature and time on TPC, FRAP and TEAC values, were assessed using single-factor tests. Three parameters with obvious effects on antioxidant capacity were selected to further investigate their interactions by response surface methodology. The optimal extraction parameters of natural antioxidants from Akebia trifoliata peels were ethanol concentration, 49.61% (v/v); solvent-to-material ratio, 32.59:1 mL/g; extraction time, 39.31 min; microwave power, 500 W; and extraction temperature, 50 °C. Under optimal conditions, the FRAP, TEAC and TPC values of Akebia trifoliata peel extracts were 351.86 ± 9.47 µM Fe(II)/g dry weight (DW), 191.12 ± 3.53 µM Trolox/g DW and 32.67 ± 0.90 mg gallic acid equivalent (GAE)/g DW, respectively. Furthermore, the main bioactive compounds (chlorogenic acid, rutin and ellagic acid) in the extract were determined by high-performance liquid chromatography. The results are useful for the full utilization of the by-product from Akebia trifoliate fruit.

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Section: Resultsmentioning

confidence: 99%

Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from Akebia trifoliata Peels

Luo

Zhou

Shang

et al. 2021

Foods

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“…Our finding is consistent with a previous study, which showed that rutin prevents glutamate-induced cell death by increasing EAAT1 and GS expression in rat brain organotypic cultures. 54 In addition, alterations in the subunit expression of NMDA and AMPA glutamate receptors have previously been found in the hippocampi of animal models of epilepsy, 25,55 in particular, the AMPA receptor GluA2 and NMDA receptor GluN2A or GluN2B subunits. [56][57][58] The AMPA receptors containing GluA2 subunits are Ca 2+ impermeable.…”

Section: Discussionmentioning

confidence: 96%

Rutin prevents seizures in kainic acid-treated rats: evidence of glutamate levels, inflammation and neuronal loss modulation

Chang

Wang

2022

Food Funct.

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“…PI labelling was used as measure of cell death as previously described [21], following treatment of organotypic slices with glutamate and/or EDAC, by incubation for 30 min with 5 µg/mL PI in aCSF at 37ºC and in a humidi ed atmosphere of 5%CO 2 . After incubation, slices were imaged using a Nikon Eclipse TE 300 Inverted uorescence microscope (Nikon Instruments Inc., Americas) with a x4 objective and CCD camera (Visitron Systems, Puchheim, Germany).…”

Section: Propidium Iodide (Pi) Labellingmentioning

confidence: 99%

“…Uptake of radiolabeled glutamate was measured as previously described [21]. Hippocampal slices (n = 6) from rats aged 90 days old were rst incubated for 1h at 37°C in HBSS containing EDAC (1µg/mL or 10 µg/mL) or DMSO vehicle in controls, followed by 1h in HBBS containing elevated glutamate (3mM) with or without EDAC (1 or 10 µg/mL).…”

Section: Glutamate Uptake In Hippocampal Brain Slicesmentioning

confidence: 99%

“…Furthermore, depending on the context, there may be either up-or down-regulation of astrocyte glutamate homeostasis, speci cally altered expression of glutamine synthetase (GS), glutamate transporter 1 (GLT1) and glutamate aspartate transporter (GLAST) [18,19]. Hence, modi cation of astrocyte responses to glutamate excitotoxicity has considerable therapeutic potential for protecting the brain in multiple pathologies and there is increasing interest in natural products in this context [20,21].…”

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Amburana cearensis seed extract stimulates astrocyte glutamate homeostatic mechanisms in hippocampal brain slices and protects oligodendrocytes against ischemia

Ferreira

Ribeiro

Silva

et al. 2022

Preprint

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Background. Stroke is a leading cause of death and disability worldwide. A major factor in brain damage following ischemia is excitotoxicity caused by elevated levels of the neurotransmitter glutamate. In the brain, glutamate homeostasis is a primary function of astrocytes. Amburana cearensis has long been used in folk medicine and seed extract obtained with dichloromethane (EDAC) have previously been shown to exhibit cytoprotective activity in vitro. The aim of the present study was to analyse the activity of EDAC in hippocampal brain slices. Methods. We prepared a dichloromethane extract (EDAC) from A. cearensis seeds and characterized the chemical constituents by 1H and 13C-NMR. Hippocampal slices from P6-8 or P90 Wistar rats were used for cell viability assay or glutamate uptake test. Hippocampal slices from P10-12 transgenic mice SOX10-EGFP and GFAP-EGFP and immunofluorescence for GS, GLAST and GLT1 were used to study oligodendrocytes and astrocytes. Results. Astrocytes play a critical role in glutamate homeostasis and we provide immunohistochemical evidence that in excitotoxicity EDAC increased expression of glutamate transporters and glutamine synthetase, which is essential for detoxifying glutamate. Next, we directly examined astrocytes using transgenic mice in which glial fibrillary acidic protein (GFAP) drives expression of enhanced green fluorescence protein (EGFP) and show that glutamate excitotoxicity caused a decrease in GFAP-EGFP and that EDAC protected against this loss. This was examined further in the oxygen-glucose deprivation (OGD) model of ischemia, where EDAC caused an increase in astrocytic process branching, resulting in an increase in GFAP-EGFP. Using SOX10-EGFP reporter mice, we show that the acute response of oligodendrocytes to OGD in hippocampal slices is a marked loss of their processes and EDAC protected oligodendrocytes against this damage. Conclusion. This study provides evidence that EDAC is cytoprotective against ischemia and glutamate excitotoxicity by modulating astrocyte responses and stimulating their glutamate homeostatic mechanisms.

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Rutin improves glutamate uptake and inhibits glutamate excitotoxicity in rat brain slices

Cited by 11 publications

References 46 publications

Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from Akebia trifoliata Peels

Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from Akebia trifoliata Peels

Rutin prevents seizures in kainic acid-treated rats: evidence of glutamate levels, inflammation and neuronal loss modulation

Amburana cearensis seed extract stimulates astrocyte glutamate homeostatic mechanisms in hippocampal brain slices and protects oligodendrocytes against ischemia

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