Rutin ameliorates cisplatin-induced reproductive damage via suppression of oxidative stress and apoptosis in adult male rats

Abstract

Cisplatin (CP) treatment causes damage in the male reproductive system. Rutin (RUT) is a naturally occurring flavonoid glycoside that has antioxidant and anti-inflammatory properties. This study aimed to investigate effects of RUT against cisplatin-induced reproductive toxicity in male rats. Twenty-one adult male Sprague Dawley rats were used. The control group received physiological saline with oral gavage during 14 days, and physiological saline was injected intraperitoneally (IP) in 10th days of study. CP Group received physiological saline during 14 days, and 10 mg kg CP was injected IP in 10th day. RUT + CP group received RUT (150 mg kg ) during 14 days, and 10 mg kg CP was injected IP in 10th day. Spermatological parameters (including motility, cauda epididymal sperm density, dead sperm percentage and morphological sperm abnormalities), biochemical (MDA, GSH, GSH-px, SOD and CAT), histological (H&E dye) and immunochemistry evaluations of testicles were evaluated. CP treatment caused damage on some spermatological parameters, increased the oxidative stress and induced testicular degeneration and apoptosis when compared to the control group. However, RUT treatment mitigates these side effects when compared to the CP alone group. IT is concluded that RUT treatment may reduce CP-induced reproductive toxicity as a potential antioxidant compound.

Protective effect of rutin on mercuric chloride‐induced reproductive damage in male rats

“…Mercury is a proxidant that causes oxidative stress (El‐Desoky et al, ; Ibrahim et al, ; Khan et al, ). Pathological events in the reproductive system can be induced by oxidative stress (Aksu, Kandemir, Kucukler, & Mahamadu, ; Aksu et al, ). Oxidative stress occurs due to excessive increase in reactive oxygen species depending on the deterioration of oxidant–antioxidant balance in the body (Ak & Gulcin, ; Aksu et al, ; Bursal & Gülçin, ; Gulcin, ).…”

“…Pathological events in the reproductive system can be induced by oxidative stress (Aksu, Kandemir, Kucukler, & Mahamadu, ; Aksu et al, ). Oxidative stress occurs due to excessive increase in reactive oxygen species depending on the deterioration of oxidant–antioxidant balance in the body (Ak & Gulcin, ; Aksu et al, ; Bursal & Gülçin, ; Gulcin, ). Due to oxidative stress, damage occurs in lipids, proteins and DNA, which are the macromolecules that make up the cell; that is why, cell integrity is impaired (Aksu et al, ).…”

“…What makes flavonoids popular include anti-inflammatory, antiallergic, antiviral, antibacterial, antitumour and antioxidant capabilities (Celik et al, 2019;Kamel, Abd El-Raouf, Metwally, Abd El-Latif, & El-sayed, 2014;Mohammad et al, 2017). Rutin with 3,3′,4′,5,7-pentahydroxyflavone-3-rhamnoglucoside chemical structure is among important flavonoids (Aksu et al, 2017). Rutin, a flavon derivate, comprising of flavonol quercetin and disaccharide rutinose has many useful biological properties such as the antioxidant, antiallergic, antibacterial, antiulcer, anti-carcinogenic, anti-inflammatory, anti-diabetic and anti-mutagenic, vasodilatory, immunomodulator and hepatoprotective (Hussein et al, 2019;Kamel et al, 2014;Kandemir et al, 2015;Stanely Mainzen Prince & Karthick, 2007).…”

“…Rutin, a flavon derivate, comprising of flavonol quercetin and disaccharide rutinose has many useful biological properties such as the antioxidant, antiallergic, antibacterial, antiulcer, anti-carcinogenic, anti-inflammatory, anti-diabetic and anti-mutagenic, vasodilatory, immunomodulator and hepatoprotective (Hussein et al, 2019;Kamel et al, 2014;Kandemir et al, 2015;Stanely Mainzen Prince & Karthick, 2007). Buckwheat, passion flower, tomato leaf, spinach, onion, apple and tea are among the plants including rutin (Aksu et al, 2017). In the studies conducted to investigate the protective properties of rutin, it has been reported that the rutin protects the kidneys in reperfusion-induced kidney damage (Korkmaz & Kolankaya, 2010).…”

Palliative effect of curcumin on doxorubicin‐induced testicular damage in male rats

“…A rich composition of polyunsaturated fatty acids in the mammalian sperm cell membrane makes the cell sensitive to free radicals. There are many experimental studies reporting that increased ROS levels decrease sperm motility and quality and increase the rate of sperm death in the semen . Using antioxidant compounds may be a useful and important strategy for preventing male infertility caused by chemotherapy.…”