Rutile Titanium Dioxide Facet Heterojunction Nanostructure with Double-Stranded Specific Nuclease for Photoelectrochemical MicroRNA-155 Detection

Abstract

In this article, a quick and sensitive photoelectrochemical biosensing platform was successfully fabricated for microRNA (miRNA)-155 detection based on the rutile titanium dioxide facet heterostructure (FH-TiO2). Nanosheets with different crystal faces (111) and (101) exposed are assembled on rutile TiO2 nanorods (TiO2 NRs) to serve as a TiO2 facet heterostructure (FH-TiO2) platform. In this assay, double-stranded specific nuclease was used to remove the double strands formed by the target RNA and probe DNA and release the target DNA for the next cycle so that the subsidiary DNA (S1) can hybridize with the residual base strands of probe DNA, which will introduce the SiO2 nanoparticles attached to the electrode surface. Infinitesimal targets could initiate huge change in the electrode signal via an enzyme-assisted target cycling procedure. A satisfactory performance with a linear range (0.1 pM to 10 nM) and the detection limit (0.025 pM, LOD = 3*σ/S) was obtained for the miRNA-155 detection, implying a promising application in the biological analysis of related diseases and clinical diagnosis.

CdSe-Co3O4@TiO2 nanoflower–based photoelectrochemical platform probing visible light–driven virus detection

“…Titanium dioxide (TiO 2 ) is widely used in PEC biosensors due to its chemical stability, photocatalytic activity, and environmental friendliness [14,15]. However, it suffers from the rapid interacting photogenerated charges, impeding the progress of TiO 2 -based PEC devices.…”

Ho2O3-TiO2 Nanobelts Electrode for Highly Selective and Sensitive Detection of Cancer miRNAs

“…Nanomaterials have broad applications in the life sciences due to their unique physical and chemical properties and structural characteristics. Of these, titanium dioxide (TiO 2 ) nanostructures have been exploited for the sensitive, accurate, rapid, and simple detection and diagnosis of cancer, e.g., nano-p–n junction heterostructures, NiO/TiO 2 nanobelts for the detection of anticancer drugs and biointeractions with cancer cells [ 6 ], Au/TiO 2 bi-heterostructured nanobelts for the specific sensing of diverse tumour cells [ 7 ], mesoporous TiO 2 for the sensing of pancreatic cancer miR-1290 [ 8 ], rutile TiO 2 facet heterojunction nanostructure nuclease for the detection of miR-155 [ 9 ], and TiO 2 nanotube arrays for the detection of prostate protein antigen [ 10 ], among others.…”