Ruthenium Complex Improves the Endothelial Function in Aortic Rings From Hypertensive Rats

Abstract

BackgroundThe endothelium is a monolayer of cells that extends on the vascular inner surface, responsible for the modulation of vascular tone. By means of the release of nitric oxide (NO), the endothelium has an important protective function against cardiovascular diseases.ObjectiveVerify if cis- [Ru(bpy)2(NO2)(NO)](PF6)2 (BPY) improves endothelial function and the sensibility of conductance (aorta) and resistance (coronary) to vascular relaxation induced by BPY.MethodsNormotensive (2K) and hypertensive (2K-1C) Wistar rats were used. For vascular reactivity study, thoracic aortas were isolated, rings with intact endothelium were incubated with: BPY(0.01 to10 µM) and concentration effect curves to acetylcholine were performed. In addition, cumulative concentration curves were performed to BPY (1.0 nM to 0.1 µM) in aortic and coronary rings, with intact and denuded endothelium.ResultsIn aorta from 2K-1C animals, the treatment with BPY 0.1µM increased the potency of acetylcholine-induced relaxation and it was able to revert the endothelial dysfunction. The presence of the endothelium did not modify the effect of BPY in inducing the relaxation in aortas from 2K and 2K-1C rats. In coronary, the endothelium potentiated the vasodilator effect of BPY in vessels from 2K and 2K-1C rats.ConclusionOur results suggest that 0.1 µM of BPY is able to normalize the relaxation endothelium dependent in hypertensive rats, and the compound BPY induces relaxation in aortic from normotensive and hypertensive rats with the same potency. The endothelium potentiate the relaxation effect induced by BPY in coronary from normotensive and hypertensive rats, with lower effect on coronary from hypertensive rats.

Do Thirty-Second Post-activation Potentiation Exercises Improve the 50-m Freestyle Sprint Performance in Adolescent Swimmers?

“…A second potential explanation for the lack of a performance enhancing effect is that the load of the 30-s PAP used in this investigation was not enough to create a potentiation effect. This may be associated with neuromuscular and molecular changes induced by strength and power activities such as: (1) better recruitment of motor units, especially type-IIx motor units, which have a higher contraction velocity and capacity to generate tension; (2) increased availability of Ca ++ for contraction; and (3) increased phosphorylation of light myosin chains, facilitating the interaction of contractile filament ( Batista et al, 2010 ; Macintosh et al, 2012 ). These mechanisms are more effective in type-IIx units, which help explaining the manifestation of PAP in trained athletes ( Xenofondos et al, 2010 ).…”

“…PAP is also associated with the close interaction between the myosin and actin. This better positioning would favor greater connections among protein filaments and, consequently, greater development of muscle tension ( Batista et al, 2010 ). In addition, it is linked to increased Ca 2+ concentration in sarcoplasm, implying greater phosphorylation of light-chain myosin, as well as greater formation of cross-bridges ( Macintosh et al, 2012 ).…”

New nitrosyl ruthenium complexes with combined activities for multiple cardiovascular disorders

“…Nitrosyl ruthenium complexes have been studied as potential pharmacological alternatives to the treatment of vascular disorders. 35,70 Since NO and HNO play major roles in cellular signaling involving pathways that lead to vasodilation, these coordination compounds can work as pro-drugs in the treatment of cardiovascular conditions, such as arterial hypertension. 9…”

“…Nitrosyl ruthenium complexes have been studied as potential pharmacological alternatives to the treatment of vascular disorders. 35,70 Since NO and HNO play major roles in cellular…”