Rupture of a Primary Aortic Aneurysm Infected with Shewanella Alga

Pocard, Marc; Grollier, G.; Moal, G. Le; Rayeh, Fatima; Camiade, Christophe

doi:10.1080/003655401317074626

Cited by 24 publications

(20 citation statements)

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“…Shewanella algae is a bacterial species recently identified (23, 24) that is closely related to Shewanella putrefaciens (formerly Pseudomonas putrefaciens) (25). Both S. putrefaciens and S. algae species are rare human pathogens (12,15,25).Recently, we have characterized ␤-lactamase OXA-54 from S. oneidensis and we have shown that this Ambler class D enzyme hydrolyzed imipenem significantly (19). Three S. algae isolates were obtained from clinical samples during the last 2 years from our hospital, and the aim of the present study was to determine their ␤-lactamase content.…”

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Genetic and Biochemical Characterization of a Chromosome-Encoded Carbapenem-Hydrolyzing Ambler Class D β-Lactamase fromShewanella algae

Héritier

Poirel

Nordmann

2004

Antimicrob Agents Chemother

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A chromosome-encoded ␤-lactamase gene from Shewanella algae clinical isolate KB-1 was cloned and expressed in Escherichia coli. It encoded the Ambler class D enzyme OXA-55, sharing less than 55% identity with any other oxacillinases. Although conferring a narrow-spectrum ␤-lactam resistance phenotype, OXA-55 had carbapenem-hydrolyzing activity that mirrored the reduced susceptibility to imipenem observed in S. algae KB-1. Very similar oxacillinases were found in other S. algae isolates.Bacteria of the genus Shewanella are gram-negative bacilli, facultatively anaerobic, belonging to the family Alteromonadaceae (25), that are widely distributed in marine and freshwater environments (23). Shewanella algae is a bacterial species recently identified (23, 24) that is closely related to Shewanella putrefaciens (formerly Pseudomonas putrefaciens) (25). Both S. putrefaciens and S. algae species are rare human pathogens (12,15,25).Recently, we have characterized ␤-lactamase OXA-54 from S. oneidensis and we have shown that this Ambler class D enzyme hydrolyzed imipenem significantly (19). Three S. algae isolates were obtained from clinical samples during the last 2 years from our hospital, and the aim of the present study was to determine their ␤-lactamase content. MATERIALS AND METHODSBacterial strains and plasmids. S. algae clinical isolate KB-1 was isolated from an arm wound of a 10-year-old girl hospitalized at the Bicêtre hospital in 2001 (Kremlin-Bicêtre, France). S. algae KB-2 and KB-3 were from a peritoneum aspiration and from a deep pus sample of the flank, respectively, from patients also hospitalized at Bicêtre hospital. These isolates were identified by the API 32GN system (bioMérieux, Marcy l'Etoile, France) and by sequencing of 16S rRNA genes using primers 16S 8-27 (5Ј-AGAGTTTGATCHTGGYTYAGA-3Ј; H is A, T, or C and Y is C or T) and 16S 1512-1491 (5Ј-ACGGYTACCTTGT TACGACTTC-3Ј; Y is C or T) (9,11,23).S. putrefaciens CIP 8040 (Institut Pasteur strain collection, Paris, France) was used as reference strain. Escherichia coli reference strain DH10B, and plasmid pBK-CMV (Stratagene, Amsterdam, The Netherlands) were used for cloning experiments, whereas rifampin-resistant E. coli JM109 was used for conjugation experiments (3).Antimicrobial agents and MIC determinations. The agents and their sources have been referenced elsewhere (17). MICs were determined by an agar dilution technique on Mueller-Hinton agar (Sanofi Diagnostics Pasteur, Paris, France) with an inoculum of 10 4 CFU per spot and were interpreted according to the guidelines of the National Committee for Clinical Laboratory Standards (14).Plasmid DNA content and conjugation. Plasmid DNA extraction of S. algae KB-1 was performed according to different methods as previously described (18). Direct transfer of ␤-lactam resistance markers into rifampin-resistant E. coli JM109 was attempted by liquid and solid mating-out assays and by electroporation of a putative plasmid DNA suspension of S. algae KB-1 into E. coli DH10B. Transconjugants and electroporants were...

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Genetic and Biochemical Characterization of a Chromosome-Encoded Carbapenem-Hydrolyzing Ambler Class D β-Lactamase fromShewanella algae

Héritier

Poirel

Nordmann

2004

Antimicrob Agents Chemother

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“…S. algae is considered a rare opportunistic pathogen for humans, frequently involving immunocompromised hosts (6,8,9), and are usually part of a polymicrobial infection (6,10,11), which may mask its clinical importance. The presence of chronic leg ulcers in the context of peripheral vascular disease occurs commonly in adults with S. algae SSTI (6,10,12,13), and the affi nity of S. algae for necrotic or ischemic tissues has been well described (12,14,15).…”

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confidence: 99%

Skin and Soft Tissue Infections (Patera Foot) in Immigrants, Spain

Vega¹,

Ángel-Moreno²,

Hernández-Cabrera³

et al. 2009

Emerg. Infect. Dis.

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“…This condition was described in two patients with lower leg ulcers (5). Three other cases of bacteremia have been reported: a patient on hemodialysis developed a case of mixed bacteremia (Escherichia coli and S. algae) (7), an immunocompromised patient with multiple myeloma developed cellulitis in both forearms in association with S. algae bacteremia (9), and a patient suffered a rupture of a primary aneurysm infected with S. algae (13). Ear infections are more common, and a series of 65 cases has been described: patients, mostly children, had acute or chronic otitis media that occurred in the summer (6).…”

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First Case of Osteomyelitis Due to Shewanella algae

et al. 2005

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Shewanella spp. are infrequently recovered from clinical specimens. We report here on the first case of osteomyelitis due to Shewanella algae. This bacterium, at first misidentified by phenotypic tests as Shewanella putrefaciens, was subsequently identified correctly as S. algae by 16S rRNA gene sequence analysis. CASE REPORTA 45-year-old woman who lived in Turkey and who had a history of depression tried to commit suicide in June 2003 by throwing herself into an open well. She broke her right tibia, resulting in an open fracture. Treatment of the fracture included osteosynthesis. No information about the initial prophylactic antibacterial therapy was available for this report. Following treatment, the initial outcome was favorable and the patient was capable of walking within the expected time frame. Ten months later, however, she presented with severe pain and limited use of her leg. She had a low-grade fever (temperature, 38°C). Her right leg was inflamed, but no fistula was present. Biochemical tests showed that her C-reactive protein level was elevated (47 mg/liter; normal range, Ͻ10 mg/liter) and her erythrocyte sedimentation rate was elevated (72 mm; normal range, Ͻ10 mm) at presentation. Radiographs of the affected leg revealed delayed consolidation of the bone with pseudarthrosis. An osteotomy and intramedullary pinning of the tibia were performed, and specimens of the removed bone and intramedullary tissue were cultured. A direct Gram stain was performed on this material but did not reveal any bacteria. Samples were inoculated onto chocolate agar plates (bioMerieux Inc., Marcy l'Etoile, France) and incubated at 37°C in a 5% CO 2 atmosphere. The samples were also inoculated onto 5% sheep blood agar (bioMerieux Inc.) and incubated under anaerobic conditions at 37°C, although prereduced medium was not used for anaerobic cultures. Fungal and mycobacterial cultures were performed but showed no growth. After 2 days, small brown mucoid colonies were observed on both agar plates (approximately 10 4 CFU per plate) and exhibited beta-hemolysis on the sheep blood agar. On a Gram stain, the isolate was found to be a gram-negative rod. The bacteria were isolated from the osteomyelitis tissue in pure culture and were identified as Shewanella putrefaciens, to which they had 99% similarity, by using the API 20NE system (bioMerieux Inc.). To further characterize the isolate, we extracted the nucleic acids from harvested colonies using a MagNA Pure kit (LC Roche,

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Rupture of a Primary Aortic Aneurysm Infected with Shewanella Alga

Abstract: Bacteria of the genus Shewanella are rarely implicated in bacteremia. We report a case of rupture of a primary aneurysm infected with Shewanella alga.

Cited by 24 publications

References 7 publications

Genetic and Biochemical Characterization of a Chromosome-Encoded Carbapenem-Hydrolyzing Ambler Class D β-Lactamase fromShewanella algae

Genetic and Biochemical Characterization of a Chromosome-Encoded Carbapenem-Hydrolyzing Ambler Class D β-Lactamase fromShewanella algae

Skin and Soft Tissue Infections (Patera Foot) in Immigrants, Spain

First Case of Osteomyelitis Due to Shewanella algae

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