Runx3 and T-box proteins cooperate to establish the transcriptional program of effector CTLs

Abstract

Activation of naive CD8 + T cells with antigen induces their differentiation into effector cytolytic T lymphocytes (CTLs

Dephosphorylation of the nuclear factor of activated T cells (NFAT) transcription factor is regulated by an RNA-protein scaffold complex

“…1B). Sizeexclusion chromatography of hypotonic lysates of murine CD8 þ T cells, activated with anti-CD3 and anti-CD28 antibodies 6 d previously and then expanded in Interleukin-2 (IL-2) (28,29), confirmed that endogenous NFAT1 coeluted with IQGAP1, IQGAP2, calmodulin, and a substantial fraction (approximately 40%) of CK1ϵ (Fig. 1C).…”

“…IQGAP1 is abundant in the cytoplasm of CD8 þ T cells, and a fraction shows regulated localization to the outer boundary of the cell-cell interface when cytolytic CD8 þ T cells encounter target cells (31). CD8 þ T cells were isolated from wild-type and Iqgap1 mutant mice and differentiated to yield functional cytolytic T cells (28,29). The cells were restimulated for 6 h with PMA plus a limiting concentration of ionomycin (250 nM), and analyzed for IFNγ expression by intracellular staining and flow cytometry.…”

“…CD8 þ T cells were purified by magnetic bead negative selection (Dynal) from spleen and axillary, brachial and inguinal lymph nodes of C57BL/6 mice (6-8 wk old). T cell differentiation was induced as described (28,29) for 6 d in 100 units per milliliter IL-2.…”

Dissecting the dynamic changes of 5-hydroxymethylcytosine in T-cell development and differentiation

“…6, Middle). Finally Runx3, a lineage-specifying factor for CD8 + cytolytic effector T cells (43), is highly expressed in CD8 SP cells but poorly expressed in naive CD8 + T cells; however, gene-body 5hmC levels were comparably high in these two cell types (Fig. 6, Bottom), perhaps because naive CD8 + T cells are the immediate precursors of cytolytic effector T cells that use Runx3 to control transcription of several effector proteins (43).…”

T-box transcription factor T-bet, a key player in a unique type of B-cell activation essential for effective viral clearance

“…T-bet, in addition to being a Th1 lineage defining transcription factor, is expressed in other cell types such as dendritic cells (7), natural killer (NK) cells (8,9), CD8 T cells (10,11), and B cells (12)(13)(14). Several reports have described the role of T-bet in B cells, mainly focusing on its requirement for class switching to IgG2a and the appearance of IgG2a-expressing memory B cells (5,6,14).…”