RUNX2 and SOX9 protein mobility correlates to osteogenic and chondrogenic differentiation of mesenchymal stem cells

Khurana, Sakshi; Govindaraj, Kannan; Karperien, M.; Post, Janine N.

doi:10.1016/j.joca.2018.02.239

Cited by 3 publications

(2 citation statements)

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“…Interestingly, earlier reports have shown that Sox9 directs chondrogenic differentiation by blocking osteogenic differentiation [24,25]. Here, we have found that Sox9 mRNA expression is reduced after osteogenic induction in AVICs.…”

Section: Marker Gene Expression Analyses In Avics Uponsupporting

confidence: 50%

Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation In Vitro

Polley

Khanam

Sengupta

et al. 2020

International Journal of Cell Biology

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Worldwide, calcific aortic valve disease is one of the leading causes of morbidity and mortality among patients with cardiac abnormalities. Aortic valve mineralization and calcification are the key events of adult calcific aortic valve disease manifestation and functional insufficiency. Due to heavy mineralization and calcification, adult aortic valvular cusps show disorganized and dispersed stratification concomitant with deposition of calcific nodules with severely compromised adult valve function. Interestingly, shared gene regulatory pathways are identified between bone-forming cells and heart valve cells during development. Asporin, a small leucine-rich proteoglycan (43 kDa), acts to inhibit mineralization in periodontal ligament cells and is also detected in normal murine adult aortic valve leaflets with unknown function. Therefore, to understand the Asporin function in aortic cusp mineralization and calcification, adult avian aortic valvular interstitial cell culture system is established and osteogenesis has been induced in these cells successfully. Upon induction of osteogenesis, reduced expression of Asporin mRNA and increased expression of bone and osteogenesis markers are detected compared to cells maintained without osteogenic induction. Importantly, treatment with human recombinant Asporin protein reduces the mineralization level in osteogenic media-induced aortic valvular interstitial cells with the concomitant decreased level of Wnt/β-catenin signaling. Overall, all these data are highly indicative that Asporin might be a novel biomolecular target to treat patients of calcific aortic valve disease over current cusp replacement surgery.

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Section: Marker Gene Expression Analyses In Avics Uponsupporting

confidence: 50%

Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation In Vitro

Polley

Khanam

Sengupta

et al. 2020

International Journal of Cell Biology

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“…Indeed, our RNA-seq and proteomic data show that during the osteoblastic differentiation treatment, the expression of these specific markers plateaued simultaneously as the expression of essential osteoblastic markers peaked. Early osteoblastic differentiation includes down-regulation of SOX9 and up-regulation of RUNX2 and ALPL ( Khurana et al, 2018 ). RUNX2 is essential for bone mineralization ( Huang et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

A multi-omics study to characterize the transdifferentiation of human dermal fibroblasts to osteoblast-like cells

Pihlström

Määttä

Öhman

et al. 2022

Front. Mol. Biosci.

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Graphical AbstractIn this study, we developed an in vitro technique to transdifferentiate human dermal fibroblasts into osteoblast-like cells. Bone marrow-derived mesenchymal stem cells, the true progenitors of osteoblasts, were utilized as reference cell lines. We characterized the osteoblastic differentiation in the two cell types using transcriptomics and quantitative MS-based proteomics and phosphoproteomics. The osteoblastic-like phenotype was confirmed in both cell types by positive alkaline phosphatase and mineralization staining methods. In addition, both cell types showed altered gene and protein profiles in compliance with an osteoblastic differentiation.

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Label-free separation of mesenchymal stem cell subpopulations with distinct differentiation potencies and paracrine effects

Yin

Yang

et al. 2020

Biomaterials

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RUNX2 and SOX9 protein mobility correlates to osteogenic and chondrogenic differentiation of mesenchymal stem cells

Cited by 3 publications

References 0 publications

Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation In Vitro

Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation In Vitro

A multi-omics study to characterize the transdifferentiation of human dermal fibroblasts to osteoblast-like cells

Label-free separation of mesenchymal stem cell subpopulations with distinct differentiation potencies and paracrine effects

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