Ruminal muscle of sheep is innervated by non-polarized pathways of cholinergic and nitrergic myenteric neurones

Pfannkuche, Helga; Schemann, Michael; Gäbel, Gotthold

doi:10.1007/s00441-002-0554-7

Cited by 33 publications

(57 citation statements)

References 27 publications

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“…We consider that the ENS cells stained with the general markers (NSE and Hu) probably represent a large proportion of the neuronal population on the basis of the specificity of these markers for neuron-specific protein in other species. In addition, the anti-NSE antibody is generally accepted to stain the entire neuronal population of the sheep ENS (Pfannkuche et al 2002). Our personal observations of the validity of NSE and Hu staining have shown that the anti-NSE antibody stains about 98% of the Hu-IR neurons and that 100% of the Hu-stained cells are NSE-IR, suggesting that both are good pan-neuronal markers of sheep enteric neurons.…”

Section: Whole-mountsmentioning

confidence: 92%

“…We have observed that CGRP-IR fibres make contacts with TK-IR, VIP-IR or NOS-IR neurons. TK-IR neurons seem to play a motor functional role in the sheep ENS, whereas VIP/NOS neurons seem to play an inhibitory role (Pfannkuche et al 2002) and so we can speculate that CGRP-IR Dogiel type II neurons play a physiological sensory role and act on both excitatory and inhibitory motor neurons.…”

Section: Cgrp-ir Fibresmentioning

confidence: 98%

“…Absorption tests have been previously carried out on sheep ENS for the guinea-pig anti-VIP and rat anti-substance P (SP) antibodies (Pfannkuche et al 2002) and the specificity of the mouse anti-NOS antibody was tested by the same authors. In this investigation, we carried out adsorption studies for the rabbit anti-CGRP serum.…”

Section: Specificity Of Antibodiesmentioning

confidence: 99%

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Extrinsic and intrinsic sources of calcitonin gene-related peptide immunoreactivity in the lamb ileum: a morphometric and neurochemical investigation

et al. 2005

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To investigate extrinsic origins of calcitonin gene-related peptide immunoreactive (CGRP-IR) nerve fibres in the sheep ileum, the retrograde fluorescent tracer Fast Blue (FB) was injected into the ileum wall. Sections of thoraco-lumbar dorsal root ganglia (DRG) and distal (nodose) vagal ganglia showing FB-labelled neurons were processed for CGRP immunohistochemistry. The distribution of CGRP-IR in fibres and nerve cell bodies in the ileum was also studied. CGRP-IR enteric neurons were morphometrically analysed in myenteric (MP) and submucosal plexuses (SMP) of lambs (2-4 months). Sensory neurons retrogradely labelled with FB were scattered in T5-L4 DRG but most were located at the upper lumbar levels (L1-L3); only a minor component of the extrinsic afferent innervation of the ileum was derived from nodose ganglia. In the DRG, 57% of retrogradely labelled neurons were also CGRP-IR. In cryostat sections, a dense network of CGRP-IR fibres was observed in the lamina propria beneath the epithelium, around the lacteals and lymphatic follicles (Peyer's platches), and along and around enteric blood vessels. Rare CGRP-IR fibres were also present in both muscle layers. Dense pericellular baskets of CGRP-IR fibres were observed around CGRP-negative somata. The only CGRP-IR nerve cells were well-defined Dogiel type II neurons localised in the MP and in the external and internal components of the SMP. CGRP-IR neurons in the myenteric ganglia were significantly larger than those in the submucosal ganglia (mean profile areas: about 1,400 mum(2) for myenteric neurons, 750 mum(2) for submucosal neurons). About 6% of myenteric neurons and 25% of submucosal neurons were CGRP-IR Dogiel type II neurons. The percentages of CGRP-IR neurons that were also tachykinin-IR were about 9% (MP) and 42% (SMP), whereas no CGRP-IR neurons exhibited immunoreactivity for vasoactive intestinal peptide, nitric oxide synthase or tyrosine hydroxylase in either plexus. Thus, CGRP immunoreactivity occurs in the enteric nervous system of the sheep ileum (as in human small intestine and MP of pig ileum) in only one morphologically defined type of neuron, Dogiel type II cells. These are probably intrinsic primary afferent neurons.

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Section: Whole-mountsmentioning

confidence: 92%

Section: Cgrp-ir Fibresmentioning

confidence: 98%

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Extrinsic and intrinsic sources of calcitonin gene-related peptide immunoreactivity in the lamb ileum: a morphometric and neurochemical investigation

et al. 2005

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“…The specificity of rat anti-SP was tested using the adsorption test (Pfannkuche et al, 2002) which, in past unpublished experiments, had been found to be totally colocalized with the rabbit anti-SP antibody employed (see Table 1). We did not test the specificity of rabbit anti-NF200 kDa antibody (Sigma, cod.…”

Section: Specificity Of Antibodiesmentioning

confidence: 99%

Immunohistochemical characterization of TH13‐L2 spinal ganglia neurons in sheep (Ovis aries)

Russo

Clavenzani

Mazzoni

et al. 2009

Microscopy Res & Technique

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Spinal ganglia (SG) neurons are commonly classified according to various specific features. The most widespread classification based on morphological and ultrastructural features subdivides SG neurons into light and small dark neurons. Using immunohistochemical, histochemical and lectin methods, it is possible to further subdivide the small dark neurons into two subpopulations: peptidergic and nonpeptidergic neurons. The majority of studies on SG neurons were carried out on mice and rats; there are few or no studies on large mammals. In this study, some of the widely used neuronal markers, neurofilament 200 kDa (NF200), substance P (SP), calcitonin gene-related peptide (CGRP) and isolectin B4 (IB4), were employed to characterize neuronal nitric oxide synthase (nNOS)-immunoreactivity (-IR) in sheep (Ovis aries) SG (Th13-L2) neurons. The majority of the SG neurons were IB4-labeled (79 +/- 10%), followed by NF200- (45 +/- 4%), NOS- (44 +/- 10%), SP- (42 +/- 5%) and CGRP-IR (35 +/- 7%) neurons. The triple staining experiments showed that a higher percentage (75 +/- 16%) of NOS-IR neurons bound both IB4 and CGRP, or both IB4 and SP (49 +/- 6%). The IB4 marker showed an unexpected staining pattern; in fact, IB4-labeled neurons largely colocalized with NF200, usually considered a marker of light SG neurons, and with CGRP and SP. For this reason, IB4 cannot be employed in sheep to differentiate between light and dark neurons, or between peptidergic and nonpeptidergic neurons. These results suggest the importance of being cautious when comparing data among different species.

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“…Acetylcholine as well as SP cause a contraction of the smooth muscle layers in the forestomach (Vassileva et al, 1978;Veenendaal et al, 1982). Retrograde tracing studies have obtained evidence that neurones immunoreactive for ChAT and SP directly take part in the innervation of ruminal smooth muscle layers (Pfannkuche et al, 2002). In our study most neurones that were not immunoreactive for acetylcholine expressed immunoreactivity for NOS and VIP.…”

Section: Discussionmentioning

confidence: 49%

Neurochemical coding of myenteric neurones in the forestomach of suckling and fattened lambs

2004

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The distinct functions of reticulum and reticular groove are presumably controlled by neurones of the myenteric plexus. Therefore, we analysed neurones according to their neurochemical code in suckling and fattened lambs. Most neurones in the reticulum were cholinergic, additionally expressing substance P and presumably excitatory. Nitrergic neurones were more abundant in the reticular groove. In both, reticulum and reticular groove, the proportion of nitrergic neurones increased with age with the increase to be more pronounced in the reticular groove. We conclude that the increase of inhibitory innervation might enable the forestomach to adapt to large amounts of ingesta during rumination.

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Ruminal muscle of sheep is innervated by non-polarized pathways of cholinergic and nitrergic myenteric neurones

Cited by 33 publications

References 27 publications

Extrinsic and intrinsic sources of calcitonin gene-related peptide immunoreactivity in the lamb ileum: a morphometric and neurochemical investigation

Extrinsic and intrinsic sources of calcitonin gene-related peptide immunoreactivity in the lamb ileum: a morphometric and neurochemical investigation

Immunohistochemical characterization of TH13‐L2 spinal ganglia neurons in sheep (Ovis aries)

Neurochemical coding of myenteric neurones in the forestomach of suckling and fattened lambs

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