Rumen protozoal dynamics during the transition from milk/grass to high-concentrate based diet in beef calves as affected by the addition of tannins or medium-chain fatty acids

Yuste, S.; Amanzougarene, Z.; Fuente, Gabriel de la; Vega, A. de; Fondevila, M.

doi:10.1016/j.anifeedsci.2019.114273

Cited by 15 publications

(22 citation statements)

References 41 publications

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“…Entodinium species are known starch degraders, and starch is essential for their maintenance and growth (Williams et al, 1992). A recent microscopy-based study found that the proportion of Entodinium in the total rumen protozoal community could reach 93% in the rumen of beef cattle fed a high concentrate-based diet (Yuste et al, 2019). The starch engulfed by Entodinium species was fermented into acetic acid and butyric acids as the major end products (together 80-90% of the total volatile fatty acids), accompanied by the production of lesser amounts of formic acid and propionic acid and some carbon dioxide and hydrogen (Abmu Akkada and Howard, 1960).…”

Section: Effect Of Residual Feed Intake On the Rumen Eukaryotic Micromentioning

confidence: 99%

Metatranscriptomic Profiling Reveals the Effect of Breed on Active Rumen Eukaryotic Composition in Beef Cattle With Varied Feed Efficiency

Zhang

Chen

et al. 2020

Front. Microbiol.

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Exploring the compositional characteristics of rumen eukaryotic community can expand our understanding of their role in rumen function and feed efficiency. In this study, we applied metatranscriptomics to characterize the active rumen eukaryotic community (protozoa and fungi) in beef cattle (n = 48) of three breeds [Angus (AN), Charolais (CH), and Kinsella Composite (KC)] and with divergent residual feed intake (RFI). The composition of active rumen eukaryotic microbiota was evaluated based on enriched 18S rRNAs from the metatranscriptomic datasets. At the phylum level, a total of four protozoal taxa (Ciliophora, Parabasalia, unclassified SAR, and unclassified Alveolata), six fungal taxa (Neocallimastigomycota, Basidiomycota, unclassified Fungi, Mucoromycota, Ascomycota, and Chytridiomycota), and one sister group of kingdom Fungi (unclassified Opisthokonta) were detected with relative abundances higher than 0.01% and in at least 50% of animals within each breed. Among these, Ciliophora, Parabasalia, unclassified Opisthokonta, and Neocallimastigomycota were the top four active eukaryotic phyla. At the genus level, a total of 8 ciliated protozoa, 5 flagellated protozoa, 5 anaerobic fungi, and 10 aerobic fungi taxa were detected, with unclassified Trichostomatia, Tetratrichomonas, unclassified Neocallimastigaceae, and Pleurotus being the most predominant taxa of ciliated protozoa, flagellated protozoa, anaerobic fungi, and aerobic fungi, respectively. Differential abundance analysis revealed that breed had a significant effect on the phylogenetic lineages of rumen eukaryotes, and seven fungal taxa were more abundant (linear discriminant analysis score > 2 with P < 0.05) in the rumen of KC steers than in the rumen of AN and CH steers. Although principal coordinate analysis (PCoA) revealed that the ruminal active eukaryotic profiles were not distinguishable between high-and low-RFI groups, the diversity indices, including Faith's phylogenetic diversity (PD), observed operational taxonomic units (OTUs), and Shannon index of rumen eukaryotes were higher in low-RFI steers than those in high-RFI steers. Meanwhile, the abundance of genus Entodinium and the kingdom Fungi was higher in low-RFI steers than that in high-RFI steers. This information on active rumen eukaryotic microbiota and identified differential abundance of taxa between high-and low-RFI animals suggests the possibility of improving feed efficiency through altering rumen eukaryotic microbiota.

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Section: Effect Of Residual Feed Intake On the Rumen Eukaryotic Micromentioning

confidence: 99%

Metatranscriptomic Profiling Reveals the Effect of Breed on Active Rumen Eukaryotic Composition in Beef Cattle With Varied Feed Efficiency

Zhang

Chen

et al. 2020

Front. Microbiol.

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“…Entodinium spp. (E. caudatum and E. simplex) ingest the grains rapidly in the early stages and then continue their digestion processes at a slower rate (about 3% of the initial rate) (Yuste et al, 2019). The microscopic examination of E. caudatum in the fluid collected during the in vitro trials revealed that the protozoan cells did not contain starch granules at the beginning of the incubation.…”

Section: Discussionmentioning

confidence: 95%

“…Entodinium spp. (E. caudatum and E. simplex) initially ingest grains very rapidly and this process subsequently slows down (about 3% of the initial rate) (Bełżecki et al, 2012;Yuste et al, 2019). Entodinium spp.…”

Section: Introductionmentioning

confidence: 99%

Effects of <i>Entodinium caudatum</i> monocultures in an acidotic environment on <i>in vitro</i> rumen fermentation

Alataş¹,

Arik²,

Gülşen³

et al. 2022

J. Anim. Feed Sci.

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“…According to a report, the abundance of genus Entodinium was higher in low-RFI (residual feed intake) steers than that in high-RFI steers 56 . Yuste et al 57 reported that the genus Entodinium account for 93% of the total rumen protozoal community when beef cattle fed a high concentrate based diet. Therefore, high abundance of genus Entodinium in the rumen may play an important role in regulating rumen lactic acid metabolism, stabilizing rumen fermentation and improving animal feed utilization e ciency.…”

Section: Discussionmentioning

confidence: 99%

The effects of Selenohomolanthionine supplementation on the rumen Eukaryotic Diversity of Shaanbei white cashmere wether goats

Liu

2022

Preprint

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Selenium (Se) is an important microelement for animal health. However, the knowledge about the effects of Se supplementation on rumen eukaryotic community remains less explored. In this study, the ruminal eukaryotic diversity in Shaanbei white cashmere goats fed a basal diet (0.016 mg/kg Se, control group [CG]) were compared to those animals given basal diet supplemented with different levels of organic Se in the form of Selenohomolanthionine (SeHLan), namely low Se group (LSE, 0.3 mg/kg), medium Se group (MSE, 0.6 mg/kg Se) and high Se group (HSE, 1.2 mg/kg) using 18S rRNA amplicon sequencing. Illumina sequencing generated 2,623,541 reads corresponding to 3123 operational taxonomic units (OTUs). Taxonomic analysis revealed that Eukaryota (77.95%) and Fungi (14.10%) were the dominant eukaryotic kingdom in all samples. The predominant rumen eukaryotic phylum was found to be Ciliophora (92.14%), while fungal phyla were dominated by Ascomycota (40.77%), Basidiomycota (23.77%), Mucoromycota (18.32%) and unidentified_Fungi (13.89%). The dominant eukaryotic genera were found to be Entodinium (55.44%), Ophryoscolex (10.51%) and Polyplastron (10.19%), while the fungal genera were dominanted by Mucor (15.39%), Pichia (9.88%), Aspergillu (8.24%), Malassezia (7.73%) and unidentified_Neocallimastigaceae (7.72%). The relative abundance of eukaryotic genera Ophryoscolex, Dasytricha, Enoploplastron and fungal genus Mucor were found to differ significantly among the four treatment groups (P<0.05). Moreover, Spearman correlation analysis revealed that the ciliate protozoa and fungi were negatively correlated with each other. The results of this first systematic study provided newer information about the effects of Se on rumen eukaryotic diversity patterns using 18s rRNA high-throughput sequencing technology.

show abstract

Rumen protozoal dynamics during the transition from milk/grass to high-concentrate based diet in beef calves as affected by the addition of tannins or medium-chain fatty acids

Cited by 15 publications

References 41 publications

Metatranscriptomic Profiling Reveals the Effect of Breed on Active Rumen Eukaryotic Composition in Beef Cattle With Varied Feed Efficiency

Metatranscriptomic Profiling Reveals the Effect of Breed on Active Rumen Eukaryotic Composition in Beef Cattle With Varied Feed Efficiency

Effects of <i>Entodinium caudatum</i> monocultures in an acidotic environment on <i>in vitro</i> rumen fermentation

The effects of Selenohomolanthionine supplementation on the rumen Eukaryotic Diversity of Shaanbei white cashmere wether goats

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