Rumen microbiota-host transcriptome interaction mediates the protective effects of trans-10, cis-12 CLA on facilitating weaning transition of lambs

Yang, Chunlei; Deng, Xiangfei; Lund, Peter; Liu, Haixia; Ding, Xingwang; Fu, Zhengwei; Zhang, Naifeng; Li, Jinjun; Dong, Lifeng

doi:10.1016/j.aninu.2022.11.002

Cited by 6 publications

(4 citation statements)

References 74 publications

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“…These results suggest that this milk fat depression may result from t10c12-CLA directly or other compositions induced by t10c12-CLA indirectly. Research on early-weaning lambs has revealed that t10c12-CLA can facilitate the weaning transition through the microbiota-host transcriptome interaction [19]. In the current study, maternal t10c12 understand the mechanism of action of t10c12-CLA in gender-specific and dosedependent manners.…”

Section: Discussionmentioning

confidence: 72%

Transgenic female mice producingtrans10,cis12-conjugated linoleic acid present excessive prostaglandin E2, adrenaline, corticosterone, glucagon, and FGF21

Rao

Liang

et al. 2023

Preprint

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Trans 10, cis 12-conjugated linoleic acid (t10c12-CLA) has been shown to induce body fat loss in mammals. In this study, we analysed female pai transgenic mice that produced t10c12-CLA to determine the long-term impact of this substance on the lipid metabolism of lean mammals. Phenotype profiling showed that the effects of t10c12-CLA were genotype-specific in biallelic pai/pai or monoallelic pai/wt mice compared to their wild-type littermates. In both pai genotypes, white fat was not reduced, but heat release was decreased. Specifically, pai/wt mice exhibited increased chronic FGF21 and adrenaline possibly to regulate energy homeostasis and reduce physical activities. Contrarily, pai/pai mice showed hypothermia which may be related to the overproduction of prostaglandins and adrenaline, as well as an inflammatory response. Analysis of brown adipose tissue indicated that hypothermia might conversely promote thermogenesis and lipolysis by up-regulating UCP1/2, pHSL, CPT1B, and AMPK, and down-regulating pAMPK in pai/pai mice. Moreover, pai/pai mice also showed hypertriglyceridemia and fatty liver which is associated with long-term excesses of corticosterone and glucagon. Further analysis of pai/pai livers revealed that hepatic steatosis resulted from attenuated lipid metabolism via down-regulating pAMPK and fatty acid synthase. These results suggest that the systemic impact of chronic t10c12-CLA is dose-dependent in lean female mammals, low doses of t10c12-CLA may play an active role via the FGF21 and/or adrenaline pathways while its high-dose exposure may cause hormones overproduction and pose a safety risk.

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Section: Discussionmentioning

confidence: 72%

Transgenic female mice producingtrans10,cis12-conjugated linoleic acid present excessive prostaglandin E2, adrenaline, corticosterone, glucagon, and FGF21

Rao

Liang

et al. 2023

Preprint

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“…After RNA extraction and transformation into cDNA, qPCR was performed in Roche LightCycler 480 System (Roche, Bethesda, MD, USA) with SYBR green, as previously described [ 30 ]. The data obtained were finally normalized to the expression of housekeeping gene β-actin using the 2 −∆∆Ct method.…”

Section: Methodsmentioning

confidence: 99%

“…To obtain the protein lysates, tissues ( n = 4 for each group) were homogenized and sonicated using the radio-immunoprecipitation assay buffer (Millipore, Billerica, MA, USA) with phosphatase inhibitors and protease (Roche Diagnostics, Basel, Switzerland) added. Then, immunoblots were conducted as described before [ 30 ]. The primary antibodies utilized were anti-AKT (1:1000, CST, #9272, Danvers, MA, USA), anti-phospho-AKT (Ser473) (1:2000, CST, #4060), anti-IκB alpha (1:5000, Abcam, ab32518, Cambridge, UK), anti-IκB alpha (phospho S36) (1:5000, Abcam, ab133462), anti-SAPK/JNK (1:1000, CST, #9252), anti-phospho-SAPK/JNK (Thr183/Tyr185) (1:1000, CST, #9251), anti-GLUT4 (1F8) (1:1000, CST, #2213), anti-GLUT2/SLC2A2 (1:1000, ABclonal, #A9843, Wuhan, China) and anti-β-actin (1:1000, CST, #4967).…”

Section: Methodsmentioning

confidence: 99%

“…For ITT, animals were intraperitoneally injected with insulin (0.75 U/kg body weight) after fasting for 6 h [20]. Blood samples were collected from tail veins and blood glucose levels were immediately measured before injection (t = 0 min) and at selected time points (30,60,90 and 120 min) after injection using FreeStyle Optium Neo (Abbott, Chicago, IL, USA).…”

Section: Glucose and Insulin Tolerance Testmentioning

confidence: 99%

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Conjugated Linoleic Acid Ameliorates High Fat-Induced Insulin Resistance via Regulating Gut Microbiota–Host Metabolic and Immunomodulatory Interactions

Wu,

Ye,

Deng

et al. 2024

Nutrients

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Interaction between gut microbiota, host immunity and metabolism has been suggested to crucially affect the development of insulin resistance (IR). This study aims to investigate how gut microbiota, inflammatory responses and metabolism in individuals with IR are affected by the supplementation of conjugated linoleic acid (CLA) and how this subsequently affects the pathophysiology of IR by using a high-fat diet-induced IR mouse model. Serum biochemical indices showed that 400 mg/kg body weight of CLA effectively attenuated hyperglycemia, hyperlipidemia, glucose intolerance and IR, while also promoting antioxidant capacities. Histomorphology, gene and protein expression analysis revealed that CLA reduced fat deposition and inflammation, and enhanced fatty acid oxidation, insulin signaling and glucose transport in adipose tissue or liver. Hepatic transcriptome analysis confirmed that CLA inhibited inflammatory signaling pathways and promoted insulin, PI3K-Akt and AMPK signaling pathways, as well as linoleic acid, arachidonic acid, arginine and proline metabolism. Gut microbiome analysis further revealed that these effects were highly associated with the enriched bacteria that showed positive correlation with the production of short-chain fatty acids (SCFAs), as well as the improved SCFAs production simultaneously. This study highlights the therapeutic actions of CLA on ameliorating IR via regulating microbiota–host metabolic and immunomodulatory interactions, which have important implications for IR control.

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Novel primers to identify a wider diversity of butyrate-producing bacteria

Meng,

Shu

2024

World J Microbiol Biotechnol

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Rumen microbiota-host transcriptome interaction mediates the protective effects of trans-10, cis-12 CLA on facilitating weaning transition of lambs

Cited by 6 publications

References 74 publications

Transgenic female mice producingtrans10,cis12-conjugated linoleic acid present excessive prostaglandin E2, adrenaline, corticosterone, glucagon, and FGF21

Transgenic female mice producingtrans10,cis12-conjugated linoleic acid present excessive prostaglandin E2, adrenaline, corticosterone, glucagon, and FGF21

Conjugated Linoleic Acid Ameliorates High Fat-Induced Insulin Resistance via Regulating Gut Microbiota–Host Metabolic and Immunomodulatory Interactions

Novel primers to identify a wider diversity of butyrate-producing bacteria

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