Rumen microbial diversity, enteric methane emission and nutrient utilization of crossbred Karan-Fries cattle (<i>Bos taurus</i>) and Murrah buffalo (<i>Bubalus bubalis</i>) consuming varied roughage concentrate ratio

Dixit, Sonam; Kumar, Sachin; Sharma, Ritu; Banakar, P.S.; Deb, Rajib; Tyagi, Amrish Kumar

doi:10.1080/10495398.2022.2053696

Cited by 5 publications

(6 citation statements)

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“…This finding further supports our hypothesis that adding host-specific probiotics may enhance growth performance by influencing the fecal microbial composition in buffalo calves. Our study is consistent with previous studies that indicate the essential role of these bacteria in maintaining the cattle gut health (Zhang et al 2021 ; Dixit et al 2022 ). Therefore, it suggests that PF supplementation was able to eliminate numerous pathogens present in the lumen of the hindgut compared to the control group.…”

Section: Discussionsupporting

confidence: 94%

“…Phylum level classification of the 16S rRNA gene amplicon sequencing data revealed that Bacteroidetes , Firmicutes, and Proteobacteria were the same dominant microbial taxa in the developing gut of preweaning calves as compared to the adult animal gut, which was in accordance with the previous reports (Rosa et al 2021 ; Guo et al 2022 ; Dixit et al 2022 ). It has been demonstrated that Bacteroidetes are known to play a crucial role in the degradation of starch, fiber, dietary protein, and absorption of amino acids and peptides in the intestine (Malmuthuge et al 2015 ).…”

Section: Discussionsupporting

confidence: 91%

“…At the genus level, perhaps the most interesting result for PF treatment was the significant increase in the population of the Prevotella genus compared to the control group. For instance, Prevotella can produce propionate, which serves as an important source of energy for the host (Dixit et al 2022 ). Thus, higher fecal propionate levels observed in this study may be related to greater abundance of the Prevotella genus (Guo et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

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Host-specific probiotics feeding influence growth, gut microbiota, and fecal biomarkers in buffalo calves

et al. 2022

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The current study is aimed to evaluate the effect of host-specific probiotics on the gut microbiome, performance, and select fecal biomarkers of gut health in preruminant buffalo calves. Eight Murrah buffalo calves (3–5 days old; 32.52 ± 0.43 kg average body weight (BW)) were randomly allocated into two groups as follows; 1) Group I (n = 4) fed basal diet alone (CON); 2) Group II (n = 4) supplemented with a lyophilized probiotic formulation at a dose rate of 1 g/day/head (1 × 109 CFU/g) having Limosilactobacillus reuteri BF-E7 and Ligilactobacillus salivarius BF-17 along with basal diet (PF) for 30 days. Results revealed that final BW (kg), average daily gain (g/day), average dry matter intake (g/day), and structural growth measurements were significantly (P < 0.05) increased in the probiotics supplemented group (PF) compared to the control (CON). Fecal pH, fecal moisture, and fecal score were reduced (P < 0.05) in PF than in CON. Moreover, levels of fecal propionate, lactate, and ammonia altered positively in PF compared with CON. The relative abundance of Firmicutes tended to be higher (P = 0.10) in the probiotics fed group than CON. However, the relative abundance of Proteobacteria was significantly lower (P = 0.03) for calves fed probiotics on day 15. A trend was observed in Bacteroides (P = 0.07) and Lactobacillus (P = 0.08) abundances in the feces of the PF than in CON. Overall, it can be concluded that the administration of probiotic formulations significantly improved the performance and gut health of buffalo calves via modulating the gut microbiota composition. Graphical Abstract

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Host-specific probiotics feeding influence growth, gut microbiota, and fecal biomarkers in buffalo calves

et al. 2022

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“…Kaithwas et al, (2020) reported comparable results in TDN (59.8, 41.4 and 39.6%) for berseem, wheat straw and paddy straw, respectively. The lower values of DE and ME of wheat straw have been found in the previous reports (Dixit et al, 2022).…”

Section: Tdn Rdp and Rup Of Forages And Agro-industrial Byproductssupporting

confidence: 44%

Carbohydrate and Protein Fractionations of commonly used forages and agro-industrial byproducts as per Cornell Net Carbohydrate and Protein system (CNCPS)

Patidar¹,

Dixit²,

Ghandour³

et al. 2022

JLS

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The present study was aimed to evaluate chemical composition viz. carbohydrate and protein fractions of some forages and agro-industrial byproducts commonly used for ruminant ration in tropical countries. Five forage samples namely berseem (Trifolium alexandrinum), maize (Zea mays), oat (Avena sativa), sorghum (Sorghum bicolor), pearl millet (Pennisetum glaucum); and four agro-industrial byproducts viz. sugarcane tops (Saccharum officinarum), oat hay, wheat straw (Triticum aestivum) and paddy (rice) straw (Oryza sativa) were evaluated by using Cornell net carbohydrate and protein (CNCP) system. Among various carbohydrate fractions, CA (Instantly/fast degradable fraction) was found highest in berseem (34.24%) and lowest in oat hay (2.63%); whereas CB1 values (Intermediately degradable fraction) ranged from 11.46% (berseem) to 3.73% (pearl millet). CB2 (Slowly degradable fraction) was highest in sugarcane tops (73.46%) and lowest in berseem (34.25%). CC which refers as undegradable carbohydrate fraction was found highest in oat hay (25.99%), but lowest in sugarcane tops (10.09%). On the other hand, protein fractions, PA (Instantaneously degradable) was found highest in sugarcane tops (39.79%) and the lowest value was there for paddy straw (9.79%). On the contrary, paddy straw contained highest PB3, which accounts for slowly degradable fraction of protein as 36.47% and the lowest value was in sugarcane tops (4.73%). Non-degradable fraction (PC) was found highest in wheat straw (30.66%) and lowest in oat (10.56%). The CNCP analysis dictated the nutrient utilization potential of the given forages and agro-industrial byproducts. Therefore, having lower CC fraction, sugarcane tops and sorghum may supply higher content of carbohydrates, however, lower PC content of oat and pearl millet make them good protein sources to ruminant ration.

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“…A unique composition of rumen microflora, for example, genus Oscillospira in buffalo heifers in the companion in vivo trial may also decide the adaptation efficiency of the animals (Dixit et al 2022). Nellore bulls adapted to high concentrate diets for 21 days had been observed with a larger rumen wall absorptive surface area as compared to the animals adapted for 14 days; however, after a longer feeding period up to 84 days, there was non-significant difference (Parra et al 2019).…”

Section: Resultsmentioning

confidence: 99%

Changes in in vitro rumen fermentation parameters of crossbred cattle (Bos taurus) and buffalo (Bubalus bubalis) in response to diet at different time intervals

Dixit

Kumar

Tyagi

2022

Indian J of Anim Sci

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Assessment of the diet adaptation period is critical for feeding experiments in ruminants to have significant changes in the concerned parameters. An in vitro trial was conducted to investigate the impact of the sampling at different time periods (day 0, 11, 21, and 60) to determine the adaptation period of feed treatment over the ruminal fermentation parameters in crossbred Karan-Fries cattle (Bos taurus) and Murrah (Bubalus bubalis) heifers. Two types of total mixed rations (TMRs), viz. high forage diet (HFD) having roughage and concentrate ratio (R:C) as 70:30 and high concentrate diet (HCD) having R:C as 40:60 were formulated, and fed to cattle and heifers. Rumen liquors were collected from all the animals on 0, 11, 21, and 60 days. HFD and HCD diets were incubated with strained rumen liquor collected from cattle and buffalo adopting Menke’s in vitro gas procedure. The results showed that most of the in vitro rumen fermentation parameters, viz. total volatile fatty acids (TVFAs) (mM/ml), acetate (%), propionate (%), butyrate (%), acetate propionate ratio (A:P), partitioning factor (PF) and microbial biomass production (MBP) (mg), in vitro dry matter digestibility (IVDMD) (%) and in vitro organic matter digestibility (IVOMD) (%) were significantly different at day 0 and 11 compared to day 21. Afterward, up to day 60, there were no effects reported except increased IVDMD and IVOMD for buffalo compared to day 21. As a result of our findings, it can be concluded that there are significant differences in rumen fermentation parameters at 11 and 21 days and that the time period for cattle and buffalo should not be reduced to 11 days from 21 days in order to achieve proper feed adaptation and stabilize the rumen fermentation process. Further research is needed to investigate the impacts of the adaption period at various time intervals.

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Rumen microbial diversity, enteric methane emission and nutrient utilization of crossbred Karan-Fries cattle (Bos taurus) and Murrah buffalo (Bubalus bubalis) consuming varied roughage concentrate ratio

Cited by 5 publications

References 70 publications

Host-specific probiotics feeding influence growth, gut microbiota, and fecal biomarkers in buffalo calves

Host-specific probiotics feeding influence growth, gut microbiota, and fecal biomarkers in buffalo calves

Carbohydrate and Protein Fractionations of commonly used forages and agro-industrial byproducts as per Cornell Net Carbohydrate and Protein system (CNCPS)

Changes in in vitro rumen fermentation parameters of crossbred cattle (Bos taurus) and buffalo (Bubalus bubalis) in response to diet at different time intervals

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